Functional Cerebral Neurovascular Mapping During Focused Ultrasound Peripheral Neuromodulation of Neuropathic Pain

Lee, Stephen A.; Kamimura, Hermes A. S.; Smith, Mila; Konofagou, Elisa E.

doi:10.1109/tbme.2024.3352025

Cited by 1 publication

(2 citation statements)

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“…It might be due to the relatively lower fUS sensitivity with the imaging transducer (f=15.625 MHz) used in this study. It may be addressed by using a high-frequency imaging transducer (f=40 MHz) capable of capturing smaller vessels with a diameter up to 75 µm [24], but it was not utilized in this study due to incompatible geometry of the FUS and high-frequency imaging transducers. It is intriguing to see lateralized and dose-dependent hemodynamic activation via correlation map depicted in Fig 5 and Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In sharp contrast with optical methods, fUS is capable of imaging the whole brain in 2D or 3D and, in contrast to fMRI, can operate in real time, measuring more transient signals with high spatiotemporal resolution. Recently, we demonstrated cortical hemodynamic responses in healthy and neuropathic mice in response to peripheral FUS stimulation [24]. We herein switched our interest into CNS to elucidate hemodynamics in response to central FUS stimulation.…”

Section: Introductionmentioning

confidence: 99%

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Functional ultrasound (fUS) imaging of displacement-guided focused ultrasound (FUS) neuromodulation in mice

Kim,

Kwon,

Hossain

et al. 2024

Preprint

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Focused ultrasound (FUS) stimulation is a promising neuromodulation technique with the merits of non-invasiveness, high spatial resolution, and deep penetration depth. However, simultaneous imaging of FUS-induced brain tissue displacement and the subsequent effect of FUS stimulation on brain hemodynamics has proven challenging thus far. In addition, earlier studies lack in situ confirmation of targeting except for the magnetic resonance imaging-guided FUS system-based studies. The purpose of this study is 1) to introduce a fully ultrasonic approach to in situ target, modulate neuronal activity, and monitor the resultant neuromodulation effect by respectively leveraging displacement imaging, FUS, and functional ultrasound (fUS) imaging, and 2) to investigate FUS-evoked cerebral blood volume (CBV) response and the relationship between CBV and displacement. We performed displacement imaging on craniotomized mice to confirm the in targeting for neuromodulation site. We recorded hemodynamic responses evoked by FUS and fUS revealed an ipsilateral CBV increase that peaks at 4 s post-FUS. We saw a stronger hemodynamic activation in the subcortical region than cortical, showing good agreement with the brain elasticity map that can also be obtained using a similar methodology. We observed dose-dependent CBV response with peak CBV, activated area, and correlation coefficient increasing with ultrasonic dose. Furthermore, by mapping displacement and hemodynamic activation, we found that displacement colocalizes and linearly correlates with CBV increase. The findings presented herein demonstrated that FUS evokes ipsilateral hemodynamic activation in cortical and subcortical depths and the evoked hemodynamic responses colocalized and correlate with FUS-induced displacement. We anticipate that our findings will help consolidate accurate targeting as well as an understanding of how FUS displaces brain tissue and affects cerebral hemodynamics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%