Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation

Zhao, Conghui; Yu, Zhenming; Silva, Jaime A. Teixeira da; He, Chunmei; Wang, Haobin; Si, Can; Zhang, Mingze; Zeng, Danqi; Duan, Jun

doi:10.3390/ijms21197005

Cited by 31 publications

(19 citation statements)

References 47 publications

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“…The results indicated that the expression patterns of CfTPS may be regulated by MeJA treatment and may respond to multiple environmental stresses ( Chaiprasongsuk et al, 2018 ). In recent studies, MeJA was shown to regulate TPS gene expression in D. officinale and C. sinensis ( Yu et al, 2020 ; Zhao et al, 2020 ; Zhou et al, 2020 ). However, this needs to be further investigated in C. faberi .…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi

et al. 2021

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Terpene synthases (TPSs) are essential for forming terpenes, which play numerous functional roles in attracting pollinators, defending plants, and moderating the interaction between plants. TPSs have been reported in some orchids, but genome-wide identification of terpenes in Cymbidium faberi is still lacking. In this study, 32 putative TPS genes were classified in C. faberi and divided into three subfamilies (TPS-a, TPS-b, and TPS-e/f). Motif and gene structure analysis revealed that most CfTPS genes had the conserved aspartate-rich DDxxD motif. TPS genes in the TPS-a and TPS-b subfamilies had variations in the RRX8W motif. Most cis-elements of CfTPS genes were found in the phytohormone responsiveness category, and MYC contained most of the numbers associated with MeJA responsiveness. The Ka/Ks ratios of 12/13 CfTPS gene pairs were less than one, indicated that most CfTPS genes have undergone negative selection. The tissue-specific expression patterns showed that 28 genes were expressed in at least one tissue in C. faberi, and TPS genes were most highly expressed in flowers, followed by leaves and pseudobulbs. In addition, four CfTPS genes were selected for the real-time reverse transcription quantitative PCR (RT-qPCR) experiment. The results revealed that CfTPS12, CfTPS18, CfTPS23, and CfTPS28 were mainly expressed in the full flowering stage. CfTPS18 could convert GPP to β-myrcene, geraniol, and α-pinene in vitro. These findings of CfTPS genes of C. faberi may provide valuable information for further studies on TPSs in orchids.

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Section: Discussionmentioning

confidence: 99%

“…In recent reports, DoTPS10 in D. officinale can convert GPP to linalool in vitro ( Yu et al, 2020 ). DoGES1 in D. officinale can catalyze geraniol in vitro and in vivo ( Zhao et al, 2020 ). Linalool and geraniol belong to monoterpenes which play essential roles in floral scents.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi

et al. 2021

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“…In D. officinale , with 34 TPS genes, 13 TPS genes were obtained in WGCNA analysis. The function of DoTPS10 has been verified; located in chloroplasts, DoTPS10 uniquely converted geranyl diphosphate to linalool in vitro ( Zhao et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Combined Analysis of Volatile Terpenoid Metabolism and Transcriptome Reveals Transcription Factors Related to Terpene Synthase in Two Cultivars of Dendrobium officinale Flowers

Dong

et al. 2021

Front. Genet.

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Dendrobium officinale is a kind of traditional Chinese herbal medicine. Its flowers could be used as health care tea for its aroma flavor and medicinal value. Most recent studies demonstrated that terpenoids are the main components of the aromatic compounds in the flowers, but the biosynthesis of terpenoids is poorly understood in D. officinale. In the experiment, the flowers from two cultivars of D. officinale with different smells were collected. The transcriptome analysis and combined volatile terpenoids determination were performed to identify the genes related to the biosynthesis of the terpenoids. The results showed that the different products of volatile terpenoids are α-thujene, linalool, α-terpineol, α-phellandrene, γ-muurolene, α-patchoulene, and δ-elemene in two cultivar flowers. The transcriptome analysis detected 25,484 genes in the flowers. And 18,650 differentially expressed genes were identified between the two cultivars. Of these genes, 253 genes were mapped to the terpenoid metabolism pathway. Among these genes, 13 terpene synthase (TPS) genes may have correlations with AP2/ERF, WRKY, MYB, bHLH, and bZIP transcription factors by weighted gene co-expression network analysis (WGCNA). The transcription factors have regulatory effects on TPS genes. These results may provide ideas for the terpenoid biosynthesis and regulatory network of D. officinale flowers.

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“…Among terpenes, geraniol is commercially important and is involved in plant–pollinator interaction and stress biology in nature. Zhao et al identified DoGES1 , a gene which encodes geraniol synthase, using genomic annotation data of D. officinale [ 8 ]. The authors studied the subcellular localization and functions of DoGES1 and finally concluded that DoGES1 was highly expressed in the petals of semi-open flowers and effectively controlled geraniol biosynthesis in D. officinale .…”

Section: Functional Genomics In Secondary Metabolites and Polysacchar...mentioning

confidence: 99%

Orchid Biochemistry 2.0

Chen

2022

IJMS

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Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation

Cited by 31 publications

References 47 publications

Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi

Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi

Combined Analysis of Volatile Terpenoid Metabolism and Transcriptome Reveals Transcription Factors Related to Terpene Synthase in Two Cultivars of Dendrobium officinale Flowers

Orchid Biochemistry 2.0

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