Functional Characterization of An Allene Oxide Synthase Involved in Biosynthesis of Jasmonic Acid and Its Influence on Metabolite Profiles and Ethylene Formation in Tea (Camellia sinensis) Flowers

Peng, Qiyuan; Zhou, Ying; Liao, Yinyin; Zeng, Lanting; Xu, Xinlan; Jia, Yongxia; Dong, Fang; Li, Jianlong; Tang, Jinchi; Yang, Ziyin

doi:10.3390/ijms19082440

Cited by 23 publications

(19 citation statements)

References 41 publications

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“…3C,D). This pattern is similar to observations made in Physcomitrella patens (Scholz et al, 2012), Vitis vinifera (Dumin et al, 2018) and Camelia sinensis (Peng et al, 2018).…”

Section: Resultssupporting

confidence: 90%

Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi

Serrazina

Machado

Costa³

et al. 2020

Preprint

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Allene oxide synthase (AOS) is a key enzyme of the jasmonic acid (JA) signaling pathway. The AOS gene was previously found to be upregulated in an Asian chestnut species resistant to infection by the oomycete Phytophthora cinnamomi (Castanea crenata), while lower expression values were detected in the susceptible European chestnut (Castanea sativa). Here, we report a genetic and functional characterization of the C. crenata AOS (CcAOS) upon its heterologous gene expression in a susceptible ecotype of Arabidopsis thaliana, which contains a single AOS gene. It was found that Arabidopsis plants expressing CcAOS delay pathogen progression and exhibit more vigorous growth in its presence. They also show upregulation of jasmonic acid and salicylic acid-related genes. As in its native species, heterologous CcAOS localized to plastids, as revealed by confocal imaging of the CcAOS-eGFP fusion protein in transgenic Arabidopsis roots. This observation was confirmed upon transient expression in Nicotiana benthamiana leaf epidermal cells. To further confirm a specific role of CcAOS in the defense mechanism against the pathogen, we performed crosses between transgenic CcAOS plants and an infertile Arabidopsis AOS knockout mutant line. It was found that plants expressing CcAOS exhibit normal growth, remain infertile but are significantly more tolerant to the pathogen than wild type plants. Together, our results indicate that CcAOS is an important player in plant defense responses against oomycete infection and that its expression in susceptible varieties may be a valuable tool to mitigate biotic stress responses.

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“…3C,D). This pattern is similar to observations made in Physcomitrella patens (Scholz et al, 2012), Vitis vinifera (Dumin et al, 2018) and Camelia sinensis (Peng et al, 2018).…”

Section: Resultssupporting

confidence: 90%

Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi

Serrazina

Machado

Costa³

et al. 2020

Preprint

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“…It was known that phytohormones (including JA, SA and ABA) are important in plant defences against herbivore attack 4, 68, 69 . To investigate whether these phytohormones are involved in the induction of OsHPL2 expression, we tested the expression levels of OsHPL2 at 1, 3, 6, 12 and 24 h after treatment.…”

Section: Resultsmentioning

confidence: 99%

Cloning and functional identification of a Chilo suppressalis‐inducible promoter of rice gene, OsHPL2

Wang

Han

et al. 2020

Pest Management Science

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BACKGROUNDPromoters play a key role in driving insect‐resistant genes during breeding of transgenic plants. In current transgenic procedures for breeding rice resistance to striped stem borer (Chilo suppressalis Walker, SSB), the constitutive promoter is used to drive the insect‐resistant gene. To reduce the burden of constitutive promoters on plant growth, isolation and identification of insect‐inducible promoters are particularly important. However, few promoters are induced specifically by insect feeding.RESULTSWe found rice hydroperoxide lyase gene (OsHPL2) (LOC_Os02g12680) was upregulated after feeding by SSB. We subsequently cloned the promoter of OsHPL2 and analysed its expression pattern using the β‐glucuronidase (GUS) reporter gene. Histochemical assays and quantitative analyses of GUS activity confirmed that P HPL2:GUS was activated by SSB, but did not respond to brown planthopper (Nilaparvata lugens Stål, BPH) infestation, mechanical wounding or phytohormone treatments. A series of 5′ truncated assays were conducted and three positive regulatory regions (−1452 to −1213, −903 to −624, and −376 to −176) induced by SSB infestation were identified. P2R123‐min 35S and P2TR2‐min 35S promoters linked with cry1C of transgenic plants showed the highest levels of Cry1C protein expression and SSB larval mortality.CONCLUSIONWe identified an SSB‐inducible promoter and three positive internal regions. Transgenic rice plants with the OsHPL2 promoter and its positive regions driving cry1C exhibited the expected larvicidal effect on SSB. Our study is the first report of an SSB‐inducible promoter that could be used as a potential resource for breeding insect‐resistant transgenic crops. © 2020 Society of Chemical Industry

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“…Over-expression of the flax AOS gene in potato led to about 8- to 12-fold increase in endogenous JA [ 33 ]. With CsAOS2 over-expression in tobacco, higher JA content was detected at 1 h after mechanical damage [ 34 ]. Transgenic rice with OsAOS2 exhibited abundant OsAOS2 transcripts and a higher endogenous JA level after pathogen infection [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of PtAOS1 Promoter and Three Novel Interacting Proteins Responding to Drought in Poncirus trifoliata

Xiong

et al. 2020

IJMS

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Jasmonic acid (JA) plays a crucial role in various biological processes including development, signal transduction and stress response. Allene oxide synthase (AOS) catalyzing (13S)-hydroperoxyoctadecatrienoic acid (13-HPOT) to an unstable allene oxide is involved in the first step of JA biosynthesis. Here, we isolated the PtAOS1 gene and its promoter from trifoliate orange (Poncirus trifoliata). PtAOS1 contains a putative chloroplast targeting sequence in N-terminal and shows relative to pistachio (Pistacia vera) AOS. A number of stress-, light- and hormone-related cis-elements were found in the PtAOS1 promoter which may be responsible for the up-regulation of PtAOS1 under drought and JA treatments. Transient expression in tobacco (Nicotiana benthamiana) demonstrated that the P−532 (−532 to +1) fragment conferring drive activity was a core region in the PtAOS1 promoter. Using yeast one-hybrid, three novel proteins, PtDUF886, PtDUF1685 and PtRAP2.4, binding to P−532 were identified. The dual luciferase assay in tobacco illustrated that all three transcription factors could enhance PtAOS1 promoter activity. Genes PtDUF1685 and PtRAP2.4 shared an expression pattern which was induced significantly by drought stress. These findings should be available evidence for trifoliate orange responding to drought through JA modulation.

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Functional Characterization of An Allene Oxide Synthase Involved in Biosynthesis of Jasmonic Acid and Its Influence on Metabolite Profiles and Ethylene Formation in Tea (Camellia sinensis) Flowers

Cited by 23 publications

References 41 publications

Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi

Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi

Cloning and functional identification of a Chilo suppressalis‐inducible promoter of rice gene, OsHPL2

Characterization of PtAOS1 Promoter and Three Novel Interacting Proteins Responding to Drought in Poncirus trifoliata

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