Functional characterization of GI and CO homologs from Eriobotrya deflexa Nakai forma koshunensis

Zhang, Ling; Jiang, Yuanyuan; Zhu, Yunmei; Su, Weixu; Long, Ting; Huang, Tianqi; Peng, Junbo; Yu, Hao; Lin, Shunquan; Gao, Yingxin

doi:10.1007/s00299-019-02384-3

Cited by 16 publications

(10 citation statements)

References 60 publications

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“…EjTFL1 and EjLFY were identified earliest, and EjAP1 was characterized later which was found to promote flowering [46,47]. Depending on the genome sequencing data of loquat (from our unpublished information), wild loquat flowering related genes of EdFT, EdFD, EdCO, and EdGI were separated and were identified to be related to flowering regulation [48,49]. Most recently, we found that EjSOC1s played a vital role in promoting flowering in cultivated loquat [50].…”

Section: Introductionmentioning

confidence: 95%

The Role of EjSPL3, EjSPL4, EjSPL5, and EjSPL9 in Regulating Flowering in Loquat (Eriobotrya japonica Lindl.)

Jiang

Peng

Wang

et al. 2019

IJMS

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The age pathway is important for regulating flower bud initiation in flowering plants. The major regulators in this pathway are miR156 and SPL transcription factors. To date, SPL genes have been identified in many species of plants. Loquat, as a woody fruit tree of Rosaceae, is unique in flowering time as it blooms in winter. However, the study of its SPL homologous genes on the regulation mechanism of flowering time is still limited. In this study, four SPL homologs—EjSPL3, EjSPL4, EjSPL5, and EjSPL9—are cloned from loquat, and phylogenetic analysis showed that they share a high sequence similarity with the homologues from other plants, including a highly conserved SQUAMOSA promoter binding protein (SBP)-box domain. EjSPL3, EjSPL4, EjSPL5 are localized in the cytoplasm and nucleus, and EjSPL9 is localized only in the nucleus. EjSPL4, EjSPL5, and EjSPL9 can significantly activate the promoters of EjSOC1-1, EjLFY-1, and EjAP1-1; overexpression of EjSPL3, EjSPL4, EjSPL5, and EjSPL9 in wild-type Arabidopsis thaliana can promote flowering obviously, and downstream flowering genes expression were upregulated. Our work indicated that the EjSPL3, EjSPL4, EjSPL5, and EjSPL9 transcription factors are speculated to likely participate in flower bud differentiation and other developmental processes in loquat. These findings are helpful to analyze the flowering regulation mechanism of loquat and provide reference for the study of the flowering mechanism of other woody fruit trees.

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Section: Introductionmentioning

confidence: 95%

The Role of EjSPL3, EjSPL4, EjSPL5, and EjSPL9 in Regulating Flowering in Loquat (Eriobotrya japonica Lindl.)

Jiang

Peng

Wang

et al. 2019

IJMS

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“…This unique flower development pattern is very important and interesting and may provide different perspectives for exploring the flower development pathway of Rosaceae; nonetheless, there are few reports on flower development in loquat. EjLFY, EjAP1, EjFT, and EjSOC1 have been cloned from cultivated loquat (Esumi et al, 2005;Liu et al, 2013;Reig et al, 2017;Jiang et al, 2019); and EdGI, EdCO, EdFT, and EdFD have been cloned from wild loquat Eriobotrya deflexa Nakai forma koshunensis Zhang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

EjTFL1 Genes Promote Growth but Inhibit Flower Bud Differentiation in Loquat

et al. 2020

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TERMINAL FLOWER1 (TFL1), a key factor belonging to the phosphatidyl ethanolaminebinding protein (PEBP) family, controls flowering time and inflorescence architecture in some plants. However, the role of TFL1 in loquat remains unknown. In this study, we cloned two TFL1-like genes (EjTFL1-1 and EjTFL1-2) with conserved deduced amino acid sequences from cultivated loquat (Eriobotrya japonica Lindl.). First, we determined that flower bud differentiation occurs at the end of June and early July, and then comprehensively analyzed the temporal and spatial expression patterns of these EjTFL1s during loquat growth and development. We observed the contrasting expression trends for EjTFL1s and EjAP1s (APETALA 1) in shoot apices, and EjTFL1s were mainly expressed in young tissues. In addition, short-day and exogenous GA 3 treatments promoted the expression of EjTFL1s, and no flower bud differentiation was observed after these treatments in loquat. Moreover, EjTFL1s were localized to the cytoplasm and nucleus, and both interacted with another flowering transcription factor, EjFD, in the nucleus, and EjTFL1s-EjFD complex significantly repressed the promoter activity of EjAP1-1. The two EjTFL1s were overexpressed in wild-type Arabidopsis thaliana Col-0, which delayed flowering time, promoted stem elongation, increased the number of branches, and also affected flower and silique phenotypes. In conclusion, our results suggested that EjTFL1-1 and EjTFL1-2 do not show the same pattern of expression whereas both are able of inhibiting flower bud differentiation and promoting vegetative growth in loquat by integrating GA 3 and photoperiod signals. These findings provide useful clues for analyzing the flowering regulatory network of loquat and provide meaningful references for flowering regulation research of other woody fruit trees.

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“… Lu et al (2020) further demonstrated that two of the rose CO paralogues interact and participate in the establishment of day neutrality in rose. There are indications that CO may control flowering time in other Rosaceae species including woodland strawberry ( Kurokura et al , 2017 ) and loquat ( Zhang et al , 2019 ). The Rosaceae is a major plant family including crop species as diverse as roses but also the small fruit species strawberries, raspberries, blueberries, etc., the fruit tree species apple, peach, cherry, apricot, almond, etc., and the less widespread loquat.…”

Section: Loss Of Photoperiod Sensitivity In the Rosaceae mentioning

confidence: 99%