2012
DOI: 10.1128/jb.00518-12
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Functional Characterization of the Stringent Response Regulatory GenedksAof Vibrio cholerae and Its Role in Modulation of Virulence Phenotypes

Abstract: In bacteria, nutrient deprivation evokes the stringent response, which is mediated by the small intracellular signaling molecule ppGpp. In Gram negatives, the RelA enzyme synthesizes and SpoT hydrolyzes ppGpp, although the latter protein also has weak synthetase activity. DksA, a recently identified RNA polymerase binding transcription factor, acts as a coregulator along with ppGpp for controlling the stringent response. Recently, we have shown that three genes, relA, spoT, and relV, govern cellular levels of … Show more

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Cited by 48 publications
(59 citation statements)
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“…Therefore, stringent response-mediated ctxAB gene expression is predicted to be controlled by the indirect mechanism, known as -factor competition. This hypothesis was supported by previous studies demonstrating that recruitment of alternative -factors ( 54 , 38 , 28 , and E ) affected survival and virulence under starvation and other stress conditions in V. cholerae (22,49,50).…”
Section: Discussionsupporting
confidence: 77%
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“…Therefore, stringent response-mediated ctxAB gene expression is predicted to be controlled by the indirect mechanism, known as -factor competition. This hypothesis was supported by previous studies demonstrating that recruitment of alternative -factors ( 54 , 38 , 28 , and E ) affected survival and virulence under starvation and other stress conditions in V. cholerae (22,49,50).…”
Section: Discussionsupporting
confidence: 77%
“…DksA (VC0596, 17.2 kDa) binds to RNAP and facilitates the effects of ppGpp on the transcriptional control in bacterial cells (22, 31, 36 -38). Recently, DksA has also been suggested to play a critical role in regulating V. cholerae virulence (22). To test whether CT production elicited by stringent response during anaerobic TMAO respiration was also affected by the deficiency of DksA, we constructed a dksA in-frame deletion mutant.…”
Section: Relv-mediated Stringent Response Plays a More Important Rolementioning
confidence: 99%
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“…We have (Pal et al, 2012). In this study, we have further examined the toxic effect of relV gene expression through pRelV BAD in E. coli background.…”
Section: Resultsmentioning
confidence: 99%
“…For reverse transcriptase (RT)-PCR assay of the relV gene, total cellular RNA was prepared from bacterial cells grown in LB medium to an OD 600 value of~1.0 using TRI Reagent (Sigma) essentially as described by the supplier. RT-PCR was done with the OneStep RT-PCR kit (Qiagen) essentially as described previously (Pal et al, 2012) using purified RNA and the primers 1224int-F/1224int-R (Table S1). …”
mentioning
confidence: 99%