Functional characterization of two efficient glycosyltransferases catalysing the formation of rutin from Sophora japonica L.

Abstract

Two efficient and selective glycosyltransferases were identified from Sophora japonica L. Sj3GT could regio-selectively catalyse 3-O-glucosylation of quercetin to produce isoquercitrin, and Sj6''RhaT could further catalyse its 6''-O-rhamnosylation to generate...

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“…Two glycotransferases, Sj3GT and Sj6′′RhaT from Sophora japonica L have been characterised and shown to convert quercetin to the bioflavonoid rutin 39 . 30 Glycotransferase Sj3GT was shown to regioselectively catalyse 3- O -glycosylation of quercetin, while Sj6′′RhaT catalysed 6′′- O -rhamnosylation of the resulting glucose moiety, which resulted in the formation of rutin. A chemoenzymatic approach has been used for the asymmetric synthesis of the benzylisoquinoline alkaloid, ( R )-reticuline 40 and its promorphinan product, (+)-salutaridine 41 .…”