Functional characterizations of one odorant binding protein and three chemosensory proteins from Apolygus lucorum (Meyer-Dur) (Hemiptera: Miridae) legs

Hua, Jinfeng; Zhang, Shuai; Cui, Jinjie; Wang, Daojie; Wang, Chunyi; Luo, Junyu; Lv, Limin; Ma, Yan

doi:10.1016/j.jinsphys.2013.04.013

Cited by 59 publications

(50 citation statements)

References 34 publications

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“…prolixus [12,38], 5 OBPs and 8 CSPs from the louse Pediculus humanus [39] and 19 OBPs and 13 CSPs from the pea aphid Acyrthosiphon pisum [40]. We added translated sequences from ESTs of two bugs from the Miridiae family: 13 OBP and 8 CSP sequences from Adelphocoris lineolatus [41–43] and 16 OBP and 12 CSP sequences from Apolygus lucorum [44–46]. Signal peptides were deleted before alignment using MAFFT version 7 [47] with the following options: E-INS-I with the BLOSUM62 scoring matrix and offset 0.1.…”

Section: Methodsmentioning

confidence: 99%

Under-Expression of Chemosensory Genes in Domiciliary Bugs of the Chagas Disease Vector Triatoma brasiliensis

et al. 2016

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BackgroundIn Latin America, the bloodsucking bugs Triatominae are vectors of Trypanosoma cruzi, the parasite that causes Chagas disease. Chemical elimination programs have been launched to control Chagas disease vectors. However, the disease persists because native vectors from sylvatic habitats are able to (re)colonize houses—a process called domiciliation. Triatoma brasiliensis is one example. Because the chemosensory system allows insects to interact with their environment and plays a key role in insect adaption, we conducted a descriptive and comparative study of the chemosensory transcriptome of T. brasiliensis samples from different ecotopes.Methodology/Principal FindingIn a reference transcriptome built using de novo assembly, we found transcripts encoding 27 odorant-binding proteins (OBPs), 17 chemosensory proteins (CSPs), 3 odorant receptors (ORs), 5 transient receptor potential channel (TRPs), 1 sensory neuron membrane protein (SNMPs), 25 takeout proteins, 72 cytochrome P450s, 5 gluthatione S-transferases, and 49 cuticular proteins. Using protein phylogenies, we showed that most of the OBPs and CSPs for T. brasiliensis had well supported orthologs in the kissing bug Rhodnius prolixus. We also showed a higher number of these genes within the bloodsucking bugs and more generally within all Hemipterans compared to the other species in the super-order Paraneoptera. Using both DESeq2 and EdgeR software, we performed differential expression analyses between samples of T. brasiliensis, taking into account their environment (sylvatic, peridomiciliary and domiciliary) and sex. We also searched clusters of co-expressed contigs using HTSCluster. Among differentially expressed (DE) contigs, most were under-expressed in the chemosensory organs of the domiciliary bugs compared to the other samples and in females compared to males. We clearly identified DE genes that play a role in the chemosensory system.Conclusion/SignificanceChemosensory genes could be good candidates for genes that contribute to adaptation or plastic rearrangement to an anthropogenic system. The domiciliary environment probably includes less diversity of xenobiotics and probably has more stable abiotic parameters than do sylvatic and peridomiciliary environments. This could explain why both detoxification and cuticle protein genes are less expressed in domiciliary bugs. Understanding the molecular basis for how vectors adapt to human dwellings may reveal new tools to control disease vectors; for example, by disrupting chemical communication.

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Section: Methodsmentioning

confidence: 99%

Under-Expression of Chemosensory Genes in Domiciliary Bugs of the Chagas Disease Vector Triatoma brasiliensis

et al. 2016

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“…LlinCSP10/LhesCSP10 aligned with AlinCSP3 and AlucCSP3. AlinCSP3 binds alfalfa and cotton volatiles (Gu et al ., ), whereas AlucCSP3 binds secondary cotton metabolites (Hua et al ., ). The two transcripts are also differentially expressed with AlinCSP3 most abundant in antennae (Gu et al ., ) and AlucCSP3 in female wings (Hua et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…While the potential ligand specificities of the orthologous sequences are intriguing, conclusions will require functional analyses of the Lygus CSPs. Furthermore, the variations in ligand binding reported in the A. lineolatus and A. lucorum studies (Gu et al ., ; Hua et al ., , ; Sun et al ., ) may be a result of methodological differences (the use of different fluorescent binding pocket probes) as opposed to actual biological/physiological differentiation of the phylogenetically related CSPs.…”

Section: Discussionmentioning

confidence: 99%

“…AMD02858) and an unannotated transcript shotgun assembly sequence (GASV02024394) from Notostira elongate . This deviation was not reported for CSPs from the mirids A. suturalis (Cui et al ., ) and A. lucorum (Hua et al ., , ). The absence of LlinCSP6/LhesCSP6 orthologs in those datasets may reflect spatial and/or temporal specific expression.…”

Section: Discussionmentioning

confidence: 99%

“…Functional characterization studies, however, have largely focused on putative chemosensory roles. Among mirid plant bugs, CSPs in Adelphocoris lineolatus have been reported to bind host‐related compounds and pheromonal components (Gu et al ., ; Sun et al ., ), whereas Apolygus lucorum CSPs bound both plant volatiles (Hua et al ., ) and secondary metabolites of cotton (Hua et al ., , ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and comparative analysis of transcripts encoding chemosensory proteins from two plant bugs, Lygus lineolaris and Lygus hesperus

2019

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Chemosensory proteins (CSPs) are soluble carrier proteins typically characterized by a six-helix bundle structure joined by two disulfide bridges and a conserved Cys spacing pattern (C1-X 6-8 -C2-X 16-21 -C3-X 2 -C4). CSPs are functionally diverse with reported roles in chemosensation, immunity, development, and resistance. To expand our molecular understanding of CSP function in plant bugs, we used recently developed transcriptomic resources for Lygus lineolaris and Lygus hesperus to identify 17 and 14 CSP-like sequences, respectively. The Lygus CSPs are orthologous and share significant sequence identity with previously annotated CSPs. Three of the CSPs are predicted to deviate from the typical CSP structure with either five or seven helical segments rather than six. The seven helix CSP is further differentiated by an atypical C3-X 3 -C4 Cys spacing motif. Reverse transcriptase PCR-based profiling of CSP transcript abundance in adult L. lineolaris tissues revealed broad expression for most of the CSPs with antenna specific expression limited to a subset of the CSPs. Comparative sequence analyses and homology modeling suggest that variations in the amino acids that comprise the Lygus CSP binding pockets affect the size and nature of the ligands accommodated.

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Expression and alternative splicing analysis of a large‐conductance calcium‐activated potassium channel gene in Plutella xylostella

Liu

et al. 2020

Arch Insect Biochem Physiol

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The large‐conductance calcium‐activated potassium channel (BKCa) plays an important role in the regulation of insect neural circuits and locomotion, and thus is a potential target of insecticides. In this study, iberiotoxin, an inhibitor of BKCa, was found to prolong the anesthetic time of ethyl acetate on Plutella xylostella larvae. Therefore, the coding sequence of slowpoke gene coding the alpha subunit of BKCa was cloned to investigate the function of this channel in P. xylostella, and the gene expression profile in the developmental stages and tissues was also characterized. The total length of pxslo DNA was more than 19.9 kb, which harbored four alternative splicing sites (ASP‐A, ASP‐C, ASP‐E, and ASP‐G), and the coding sequence of pxslo with the highest frequency of splicing (GenBank ID: MN938456) was 3,405 base pair. The characterized PxSlo protein contained conserved domains previously identified in other insects. Quantitative reverse transcription‐polymerase chain reaction analysis showed that pxslo was expressed in all the developmental stages of P. xylostella, with the highest level in adults. In the larval stage, pxslo was mainly expressed in the head and epidermis, while a limited protein was expressed in the midgut. In the adult stage, pxslo was highly expressed in the head, followed by in the ovarian tubule, and was not expressed in the testis or wings. These results suggest that BKCa plays an important physiological role in P. xylostella and provides useful information for the functional study and screening of BKCa inhibitors.

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Functional characterizations of one odorant binding protein and three chemosensory proteins from Apolygus lucorum (Meyer-Dur) (Hemiptera: Miridae) legs

Cited by 59 publications

References 34 publications

Under-Expression of Chemosensory Genes in Domiciliary Bugs of the Chagas Disease Vector Triatoma brasiliensis

Under-Expression of Chemosensory Genes in Domiciliary Bugs of the Chagas Disease Vector Triatoma brasiliensis

Molecular cloning and comparative analysis of transcripts encoding chemosensory proteins from two plant bugs, Lygus lineolaris and Lygus hesperus

Expression and alternative splicing analysis of a large‐conductance calcium‐activated potassium channel gene in Plutella xylostella

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