Functional diversification of tomato HsfA1 factors is based on DNA binding domain properties

El-Shershaby, Asmaa; Ullrich, Sarah; Simm, Stefan; Scharf, Klaus‐Dieter; Schleiff, Enrico; Fragkostefanakis, Sotirios

doi:10.1016/j.gene.2019.143985

Cited by 26 publications

(24 citation statements)

References 37 publications

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“…Tomato also has four HsfA1 genes, namely HsfA1a , HsfA1b , HsfA1d , and HsfA1e , in which HsfA1 was reported to be a master regulator of heat stress response [ 27 ]. Meanwhile, the expression of HsfA1a is constitutive under control and stress conditions, while the other members are induced in specific tissues and stages of heat stress response [ 28 ]. HsfA1s are early heat stress response genes activating the expression of the late response Hsf genes such as HsfA2 in Arabidopsis as well as in tomato [ 24 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

CaHsfA1d Improves Plant Thermotolerance via Regulating the Expression of Stress- and Antioxidant-Related Genes

Gai

et al. 2020

IJMS

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Heat shock transcription factor (Hsf) plays an important role in regulating plant thermotolerance. The function and regulatory mechanism of CaHsfA1d in heat stress tolerance of pepper have not been reported yet. In this study, phylogenetic tree and sequence analyses confirmed that CaHsfA1d is a class A Hsf. CaHsfA1d harbored transcriptional function and predicted the aromatic, hydrophobic, and acidic (AHA) motif mediated function of CaHsfA1d as a transcription activator. Subcellular localization assay showed that CaHsfA1d protein is localized in the nucleus. The CaHsfA1d was transcriptionally up-regulated at high temperatures and its expression in the thermotolerant pepper line R9 was more sensitive than that in thermosensitive pepper line B6. The function of CaHsfA1d under heat stress was characterized in CaHsfA1d-silenced pepper plants and CaHsfA1d-overexpression Arabidopsis plants. Silencing of the CaHsfA1d reduced the thermotolerance of the pepper, while CaHsfA1d-overexpression Arabidopsis plants exhibited an increased insensitivity to high temperatures. Moreover, the CaHsfA1d maintained the H2O2 dynamic balance under heat stress and increased the expression of Hsfs, Hsps (heat shock protein), and antioxidant gene AtGSTU5 (glutathione S-transferase class tau 5) in transgenic lines. Our findings clearly indicate that CaHsfA1d improved the plant thermotolerance via regulating the expression of stress- and antioxidant-related genes.

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Section: Introductionmentioning

confidence: 99%

CaHsfA1d Improves Plant Thermotolerance via Regulating the Expression of Stress- and Antioxidant-Related Genes

Gai

et al. 2020

IJMS

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“…AtWRKY25 can increase the contents of HsfA2, HsfB1, HsfB2A, and Hsp100F in AtWRKY25 overexpression lines of A. thaliana, which significantly improves the high-temperature resistance of the plants 59,60 . New evidence suggests that other TFs except for HSFs, for example ATHB and ERFs were also regulating plant's thermotolerance 61 . ATHB6 is a specific TF of higher plants and one of the components of the early signal transduction pathway, participating in the ABA signaling pathway as a main specific switch 62 .…”

Section: Discussionmentioning

confidence: 99%

Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis

Pei

Zhang

Zhu

et al. 2021

Sci Rep

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Sorbus pohuashanensis (Hance) Hedl. is a Chinese native alpine tree species, but the problem of introducing S. pohuashanensis to low altitude areas has not been solved. In this study, we aimed to explore the molecular regulatory network of S. pohuashanensis in response to high-temperature stress using RNA-Sequencing technology and physiological and biochemical determination. Based on transcriptomic data, we obtained 1221 genes (752 up-regulated and 469 down-regulated) that were differentially expressed during 8 h 43℃ treatment and candidate genes were related to calcium signaling pathway, plant hormone signal transduction, heat shock factors, chaperones, ubiquitin mediated proteolysis, cell wall modification, ROS scavenging enzymes, detoxification and energy metabolism. The analysis of high temperature response at the physiological level and biochemical level were performed. The chlorophyll fluorescence parameters of leaf cells decreased, the content of osmotic regulators increased, and the activity of ROS scavenging enzymes decreased. The molecular regulatory network of S. pohuashanensis in response to high-temperature stress was preliminarily revealed in this study, which provides fundamental information improving introducing methods and discovering heat-tolerant genes involved in high-temperature stress in this species and provides a reference for other plants of the genus Sorbus.

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“…Reduced activation of most heat-induced HSF genes in QK background confirm the importance of class A1 factors in stress tolerance (Figure 3). Functional diversification of class A HSFs depends on the variation of DNA binding domain (DBD), which defines the range of target genes (El-Shershaby, Ullrich, Simm, Scharf, Schleiff & Fragkostefanakis, 2019). Class A HSFs does not act alone.…”

Section: Regulation Of Responses To Abiotic Stressesmentioning

confidence: 99%

Variability of plant heat shock factors: regulation, interactions and functions.

Andrási

Pettkó‐Szandtner

Szabados

2020

Preprint

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In plants Heat Shock Factors (HSFs) are encoded by large gene families and are primary regulators of responses not only to high temperatures but also to a number of other abiotic stresses and pathogen threats. Here we provide an overview of the diverse world of the plant HSFs through analysis of their functional versatility, regulation and interactions. HSFs can regulate tolerance to a number of extreme conditions including high or low temperatures, drought, hypoxic conditions, soil salinity, toxic minerals, strong irradiation or pathogen defenses. Variability is reflected in expression control with considerable differences in transcript profiles of individual HSF genes. Moreover, alternative splicing and posttranslational modifications provides further variability. HSFs are involved in complex web of protein-protein interactions which include formation of homomeric and heteromeric HSF trimers, and complexes with a number of other regulatory proteins including transcription regulators, chromatin-associated proteins or heat shock proteins (HSPs). Interactions of the Arabidopsis HSFA4A with proteins which control transcription, cellular homeostasis, responses to different stresses and programmed cell death, illustrate the complexity of the regulatory networks related to a plant HSF. Diversity in plant HSFs facilitates the adaptation to multiple adverse environmental conditions, an important feature in response to climate change.

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Functional diversification of tomato HsfA1 factors is based on DNA binding domain properties

Cited by 26 publications

References 37 publications

CaHsfA1d Improves Plant Thermotolerance via Regulating the Expression of Stress- and Antioxidant-Related Genes

CaHsfA1d Improves Plant Thermotolerance via Regulating the Expression of Stress- and Antioxidant-Related Genes

Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis

Variability of plant heat shock factors: regulation, interactions and functions.

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