Functional electrospun fibrous scaffolds with dextran-<i>g</i>-poly(<scp>l</scp>-lysine)-VAPG/microRNA-145 to specially modulate vascular SMCs

Zhou, Peiqiong; Zhou, Fang; Liu, Bo; Zhao, Yunhui; Yuan, Xiaoyan

doi:10.1039/c7tb01755c

Cited by 32 publications

(35 citation statements)

References 46 publications

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“…VSMC proliferation on thick‐PLGAm and thin‐PLGAm was significantly lower than those without miR‐145, suggesting the depression effect of miR‐145 complexes to VSMCs. The response of VSMCs to the released miR‐145 from thick‐PLGAm and thin‐PLGAm was consistent with the biological function of miR‐145, and our report . Even though thin‐PLGAm had a faster release rate of miR‐145 than thick‐PLGAm, VSMCs showed slower proliferation on thick‐PLGAm than on thin‐PLGAm [Fig.…”

Section: Discussionsupporting

confidence: 87%

“…The response of VSMCs to the released miR-145 from thick-PLGAm and thin-PLGAm was consistent with the biological function of miR-145, and our report. [25][26][27]30 Even though thin-PLGAm had a faster release rate of miR-145 than thick-PLGAm, VSMCs showed slower proliferation on thick-PLGAm than on thin-PLGAm [ Fig. 5(a)].…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, miRNA‐145 (miR‐145) can block the target genes KLF4/5 and myocardin in VSMCs, modulating VSMCs in the normal contractile phenotype . In our previous researches, we have attempted to prepare miR‐126 and miR‐145 complexes by cationic carriers, which contained peptides Arg‐Glu‐Asp‐Val (REDV) and Val‐Ala‐Pro‐Gly (VAPG) for targeting VECs and VSMCs, respectively . The electrospun fibrous grafts individual‐loading with miR‐126 or miR‐145 complexes could regulate the physiological behaviors of VECs and VSMCs, respectively, showing the tendency to promote vascular regeneration …”

Section: Introductionmentioning

confidence: 99%

“…[24][25][26][27] In our previous researches, we have attempted to prepare miR-126 and miR-145 complexes by cationic carriers, which contained peptides Arg-Glu-Asp-Val (REDV) and Val-Ala-Pro-Gly (VAPG) for targeting VECs and VSMCs, respectively. [28][29][30] The electrospun fibrous grafts individual-loading with miR-126 or miR-145 complexes could regulate the physiological behaviors of VECs and VSMCs, respectively, showing the tendency to promote vascular regeneration. [28][29][30] During small-diameter vascular regeneration, VECs are expected to grow rapidly in the initial days to reduce thrombosis and inflammation, as well as keep long-term patency.…”

Section: Introductionmentioning

confidence: 99%

“…[28][29][30] The electrospun fibrous grafts individual-loading with miR-126 or miR-145 complexes could regulate the physiological behaviors of VECs and VSMCs, respectively, showing the tendency to promote vascular regeneration. [28][29][30] During small-diameter vascular regeneration, VECs are expected to grow rapidly in the initial days to reduce thrombosis and inflammation, as well as keep long-term patency. 6,7 In the meanwhile, proper proliferation of VSMCs is also necessary to support the vascular regeneration and provide elasticity.…”

Section: Introductionmentioning

confidence: 99%

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Target regulation of both VECs and VSMCs by dual‐loading miRNA‐126 and miRNA‐145 in the bilayered electrospun membrane for small‐diameter vascular regeneration

Cui

Wen

Zhou

et al. 2018

J Biomedical Materials Res

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Clinical utility of small‐diameter vascular grafts is still challenging in blood vessel regeneration owing to thrombosis and intimal hyperplasia. To cope with the issues, modulation of gene expression via microRNAs (miRNAs) could be a feasible approach by rational regulating physiological activities of both vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs). Our previous studies demonstrated that individually loaded miRNA‐126 (miR‐126) or miRNA‐145 (miR‐145) in the electrospun membranes showed the tendency to promote vascular regeneration. In this work, the bilayered electrospun graft in 1.5‐mm diameter was developed by emulsion electrospinning to dual‐load miR‐126 and miR‐145 for target regulation of both VECs and VSMCs, respectively. Accelerated release of miR‐126 was achieved by introducing poly(ethylene glycol) in the inner electrospun poly(ethylene glycol)‐b‐poly(l‐lactide‐co‐caprolactone) ultrafine fibrous membrane, reaching 61.3 ± 1.2% of the cumulative release in the initial 10 days, whereas the outer electrospun poly(l‐lactide‐co‐glycolide) membrane composed of microfibers fulfilled prolonged release of miR‐145 for about 56 days. In vivo tests suggested that dual‐loading with miR‐126 and miR‐145 in the bilayered electrospun membranes could modulate both VECs and VSMCs for rapid endothelialization and hyperplasia inhibition as well. It is reasonably expected that dual target‐delivery of miR‐126 and miR‐145 in the electrospun vascular grafts has effective potential for small‐diameter vascular regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 371–382, 2019.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Target regulation of both VECs and VSMCs by dual‐loading miRNA‐126 and miRNA‐145 in the bilayered electrospun membrane for small‐diameter vascular regeneration

Cui

Wen

Zhou

et al. 2018

J Biomedical Materials Res

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Surface Engineering of Cardiovascular Devices for Improved Hemocompatibility and Rapid Endothelialization

Zhao

Feng

2020

Adv Healthcare Materials

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Cardiovascular devices have been widely applied in the clinical treatment of cardiovascular diseases. However, poor hemocompatibility and slow endothelialization on their surface still exist. Numerous surface engineering strategies have mainly sought to modify the device surface through physical, chemical, and biological approaches to improve surface hemocompatibility and endothelialization. The alteration of physical characteristics and pattern topographies brings some hopeful outcomes and plays a notable role in this respect. The chemical and biological approaches can provide potential signs of success in the endothelialization of vascular device surfaces. They usually involve therapeutic drugs, specific peptides, adhesive proteins, antibodies, growth factors and nitric oxide (NO) donors. The gene engineering can enhance the proliferation, growth, and migration of vascular cells, thus boosting the endothelialization. In this review, the surface engineering strategies are highlighted and summarized to improve hemocompatibility and rapid endothelialization on the cardiovascular devices. The potential outlook is also briefly discussed to help guide endothelialization strategies and inspire further innovations. It is hoped that this review can assist with the surface engineering of cardiovascular devices and promote future advancements in this emerging research field.

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Two Sides of Electrospun Fiber in Promoting and Inhibiting Biomedical Processes

Wang

Cui

2020

Advanced Therapeutics

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Electrospinning is a versatile technique for generating ultrathin fibers, which is widely applied in various biomedical applications. The properties and structures of the fibers are specifically engineered to meet the needs of particular applications. The impact of nanofibrous scaffolds on biological processes is related to the promotion or inhibition of specific cell or bioactive substance functions. Here, a novel analysis of the diverse roles of electrospun nanofibrous scaffolds is provided and recent advances in exploiting their binary attributes for applications in biomedicine are summarized. This review initially introduces the development techniques for electrospinning, specifically the engineering of electrospun nanofibers. Then, scaffold applications for cell migration, adhesion, proliferation, and accelerating regeneration in cardiac, nerve, skeletal muscle, bone tissue, and wound healing are presented. Moreover, their inhibitory properties in cancer treatments, antibacterial applications, anti‐adhesion, anti‐scarring, and inhibition of thrombus formation are summarized. Eventually, the potential for future work using multifunctional, electrospun, nanofibrous scaffolds, in order to further inspire the development of scaffolds for biomedical treatments is summarized and discussed.

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Functional electrospun fibrous scaffolds with dextran-g-poly(l-lysine)-VAPG/microRNA-145 to specially modulate vascular SMCs

Abstract: Functional electrospun membranes loaded with Dex-g-PLL-VAPG/miR-145 complexes exhibit the excellent ability to modulate SMC phenotype and proliferation locally.

Cited by 32 publications

References 46 publications

Target regulation of both VECs and VSMCs by dual‐loading miRNA‐126 and miRNA‐145 in the bilayered electrospun membrane for small‐diameter vascular regeneration

Target regulation of both VECs and VSMCs by dual‐loading miRNA‐126 and miRNA‐145 in the bilayered electrospun membrane for small‐diameter vascular regeneration

Surface Engineering of Cardiovascular Devices for Improved Hemocompatibility and Rapid Endothelialization

Two Sides of Electrospun Fiber in Promoting and Inhibiting Biomedical Processes

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