Functional Expression and Characterization of a Truncated Candida antarctica Lipase B in Yeast

Abstract

and their respective research groups at the USDA for their collaboration and insight on this project. And finally, I would like to thank my family, friends, and especially my partner, Michael, for giving me the continuous support and encouragement needed to complete this project. S.M.R. viii 22. Michaelis-Menten plot for the substrate p-NPB ranging from 40-1000 µM and the truncated CalB enzyme using the method of Blank et al. (2006). 23. Michaelis-Menten plot for the substrate p-NPB ranging from 40-6000 µM and the truncated CalB enzyme using the method of Blank et al. (2006). 24. Michaelis-Menten plot for the substrate p-NPB ranging from 40-20000 µM and the truncated CalB enzyme using the method of Martinelle et al. (1995). 25. Michaelis-Menten plot for the substrate p-NPB ranging from 40-20000 µM and the full-length CalB enzyme using the method of Martinelle et al. (1995).