Functional Implications of Photosystem II Crystal Formation in Photosynthetic Membranes

Tietz, Stefanie; Puthiyaveetil, Sujith; Enlow, Heather M.; Yarbrough, Robert; Wood, Magnus; Semchonok, Dmitry A.; Lowry, Troy W.; Li, Zhirong; Jahns, Peter; Boekema, Egbert J.; Lenhert, Steven; Niyogi, Krishna; Kirchhoff, Helmut

doi:10.1074/jbc.m114.619841

Cited by 45 publications

(33 citation statements)

References 73 publications

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“…In this last model, a positively charged N-terminal segment of LHCII in one membrane interacts with a negatively charged stromal surface of the opposite LHCII trimer (Standfuss et al, 2005;Daum et al, 2010). Recently, however, the significance of LHCII-PSII supercomplexes and the LHCII trimer arrangement in grana membranes has been emphasized (Kim et al, 2009;Kou ril et al, 2012;Tietz et al, 2015). The contact between stacked adjacent membranes is made by the stromal surfaces of polypeptides of supercomplexes, mainly LHCII trimers, PSII core, and minor antennae (Daum et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Visualization of the Tubular-Lamellar Transformation of the Internal Plastid Membrane Network during Runner Bean Chloroplast Biogenesis

et al. 2016

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Chloroplast biogenesis is a complex process that is integrated with plant development, leading to fully differentiated and functionally mature plastids. In this work, we used electron tomography and confocal microscopy to reconstruct the process of structural membrane transformation during the etioplast-to-chloroplast transition in runner bean (Phaseolus coccineus). During chloroplast development, the regular tubular network of paracrystalline prolamellar bodies (PLBs) and the flattened porous membranes of prothylakoids develop into the chloroplast thylakoids. Three-dimensional reconstruction is required to provide us with a more complete understanding of this transformation. We provide spatial models of the bean chloroplast biogenesis that allow such reconstruction of the internal membranes of the developing chloroplast and visualize the transformation from the tubular arrangement to the linear system of parallel lamellae. We prove that the tubular structure of the PLB transforms directly to flat slats, without dispersion to vesicles. We demonstrate that the grana/stroma thylakoid connections have a helical character starting from the early stages of appressed membrane formation. Moreover, we point out the importance of particular chlorophyll-protein complex components in the membrane stacking during the biogenesis. The main stages of chloroplast internal membrane biogenesis are presented in a movie that shows the time development of the chloroplast biogenesis as a dynamic model of this process.

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Section: Discussionmentioning

confidence: 99%

Three-Dimensional Visualization of the Tubular-Lamellar Transformation of the Internal Plastid Membrane Network during Runner Bean Chloroplast Biogenesis

et al. 2016

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“…Additional LHCII trimers can be found loosely bound to the supercomplex (i.e., L-trimers) [15], whereas some others are present in the membrane not directly associated with the PSII core [10]. Within the membrane, PSII-LHCII supercomplexes may associate to form PSII-LHCII megacomplexes or even large ordered arrays [16][17][18][19].…”

Section: Introductionmentioning

confidence: 98%

Dynamic reorganization of photosystem II supercomplexes in response to variations in light intensities

Albanese

Manfredi

Meneghesso

et al. 2016

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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Plants are sessile organisms and need to acclimate to ever-changing light conditions in order to survive. These changes trigger a dynamic reorganization of the membrane protein complexes in the thylakoid membranes. Photosystem II (PSII) and its light harvesting system (LHCII) are the major target of this acclimation response, and accumulating evidences indicate that the amount and composition of PSII-LHCII supercomplexes in thylakoids are dynamically adjusted in response to changes in light intensity and quality. In this study, we characterized the PSII-LHCII supercomplexes in thylakoid membranes of pea plants in response to long-term acclimation to different light intensities. We provide evidence of a reorganization of the PSII-LHCII supercomplexes showing distinct changes in their antenna moiety. Mass spectrometry analysis revealed a specific reduction of Lhcb3, Lhcb6 and M-LHCII trimers bound to the PSII cores, while the Lhcb4.3 isoform increased in response to high light intensities. The modulation of Lhcb protein content correlates with the reduction of the functional PSII antenna size. These results suggest that the Lhcb3, Lhcb4.3 and Lhcb6 antenna subunits are major players in modulation of the PSII antenna size upon long-term acclimation to increased light levels. PsbS was not detected in the isolated PSII-LHCII supercomplexes at any light condition, despite an increased accumulation in thylakoids of high light acclimated plants, suggesting that PsbS is not a constitutive component of PSII-LHCII supercomplexes.

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“…Dudkina et al ., for review), are important for the regulation and optimization of photosynthetic processes and small protein traffic (for reviews, see e.g. Kouřil et al ., ; Kirchhoff, ; Tietz et al ., ), and may also contribute to grana formation (Daum et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Structural variability of plant photosystem II megacomplexes in thylakoid membranes

et al. 2016

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SUMMARYPlant photosystem II (PSII) is organized into large supercomplexes with variable levels of membrane-bound light-harvesting proteins (LHCIIs). The largest stable form of the PSII supercomplex involves four LHCII trimers, which are specifically connected to the PSII core dimer via monomeric antenna proteins. The PSII supercomplexes can further interact in the thylakoid membrane, forming PSII megacomplexes. So far, only megacomplexes consisting of two PSII supercomplexes associated in parallel have been observed. Here we show that the forms of PSII megacomplexes can be much more variable. We performed single particle electron microscopy (EM) analysis of PSII megacomplexes isolated from Arabidopsis thaliana using clear-native polyacrylamide gel electrophoresis. Extensive image analysis of a large data set revealed that besides the known PSII megacomplexes, there are distinct groups of megacomplexes with non-parallel association of supercomplexes. In some of them, we have found additional LHCII trimers, which appear to stabilize the non-parallel assemblies. We also performed EM analysis of the PSII supercomplexes on the level of whole grana membranes and successfully identified several types of megacomplexes, including those with nonparallel supercomplexes, which strongly supports their natural origin. Our data demonstrate a remarkable ability of plant PSII to form various larger assemblies, which may control photochemical usage of absorbed light energy in plants in a changing environment.

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Functional Implications of Photosystem II Crystal Formation in Photosynthetic Membranes

Cited by 45 publications

References 73 publications

Three-Dimensional Visualization of the Tubular-Lamellar Transformation of the Internal Plastid Membrane Network during Runner Bean Chloroplast Biogenesis

Three-Dimensional Visualization of the Tubular-Lamellar Transformation of the Internal Plastid Membrane Network during Runner Bean Chloroplast Biogenesis

Dynamic reorganization of photosystem II supercomplexes in response to variations in light intensities

Structural variability of plant photosystem II megacomplexes in thylakoid membranes

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