Functional in Vitro Analysis of the ERO1 Protein and Protein-disulfide Isomerase Pathway

Araki, Kazutaka; Nagata, Kazuhiro

doi:10.1074/jbc.m111.227181

Cited by 76 publications

(103 citation statements)

References 37 publications

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“…Numerous studies have demonstrated that ROS is important for killing tumor cells in all types of living organisms (25,26). Besides, it may act as a second messenger to regulate cell proliferation, differentiation and apoptosis, which are associated with signal transduction pathways (10). A previous study has demonstrated that the antioxidant enzyme activity within the tumor cells was lower than that within normal cells, and that the removal efficiency of ROS was also low in tumor cells, suggesting that the intracellular ROS levels are more likely to kill tumor cells (27).…”

Section: Discussionmentioning

confidence: 99%

“…ROS is a general term for reactive oxygen compounds, which are produced in the process of biological aerobic metabolism in the form of O -2 , H 2 O 2 , HO -and nitric oxide (10). Besides, it is important in cell apoptosis through various different pathways (24).…”

Section: Discussionmentioning

confidence: 99%

“…In normal cells, two redox systems exist, the peroxide enzyme system and resistance against superoxide (9). The former includes the oxygen reduction sulfur protein, while the latter includes superoxide dismutase, catalase and glutathione peroxidase (10). These systems can remove reactive oxygen species (ROS) produced in the process of metabolism, which results in relatively stable levels of intracellular ROS (11).…”

Section: Introductionmentioning

confidence: 99%

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Anticancer effect of icaritin inhibits cell growth of colon cancer through reactive oxygen species, Bcl-2 and cyclin D1/E signaling

Peng

Song

et al. 2016

Oncology Letters

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Abstract. Icaritin has an advantage in enhancing immunity. Besides, with its anticancer effect, it may be of great help in cancer treatment and recovery of cancer patients. As a result, icaritin is likely to become a novel anticancer drug. However, the anticancer effect of icaritin against colon cancer has not been elucidated thus far. The present study investigated the latent anticancer effect of icaritin on the inhibition of colon cancer cell growth by regulating reactive oxygen species (ROS), B-cell lymphoma (Bcl)-2 and cyclin D1/E signaling. The COLO-205 colon cancer cell line was used as a colon cancer cell model in the present study. First, cell growth and apoptosis were measured to analyze the anticancer effect of icaritin against colon cancer. Next, the possible mechanism of icaritin against colon cancer, including ROS, Bcl-2, cyclin D1, cyclin E and caspase-3/9, was explored. The results revealed that icaritin could inhibit cell growth and induce the apoptosis of COLO-205 cells. In addition, icaritin significantly induced ROS generation, suppressed Bcl-2, cyclin D1 and cyclin E protein expression, and activated caspase-3/9 activity in COLO-205 cells. The present findings demonstrated that icaritin exerted antiproliferative and anticancer effects against colon cancer through the activation of ROS generation and the suppression of Bcl-2, cyclin D1 and cyclin E signaling.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anticancer effect of icaritin inhibits cell growth of colon cancer through reactive oxygen species, Bcl-2 and cyclin D1/E signaling

Peng

Song

et al. 2016

Oncology Letters

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“…Thus, Ero1 activity must be regulated to prevent ROS build-up and hyperoxidizing conditions in the ER (11). Intramolecular disulfide bonds have been shown to negatively regulate the activity of both human Ero1 paralogs (10,(12)(13)(14). Although the activity of Ero1␣ is tightly controlled (12)(13)(14), Ero1␤ seems to be more loosely regulated (10,13).…”

mentioning

confidence: 99%

Hyperactivity of the Ero1α Oxidase Elicits Endoplasmic Reticulum Stress but No Broad Antioxidant Response

Hansen

Schmidt

Søltoft

et al. 2012

Journal of Biological Chemistry

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Background:The oxidase activity of human Ero1␣ generates hydrogen peroxide in the ER. Results: Overexpression of a hyperactive Ero1␣ mutant induces the unfolded protein response but does not cause a broad antioxidant response. Conclusion: Ero1␣ hyperactivity elicits ER stress through local ER lumenal hyperoxidation. Significance: These findings show how the cell negotiates oxidative stress generated specifically in the lumen of the ER.

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“…The oxidizing environment of the ER is a prerequisite for the formation of disulfide bonds and is maintained by a network of redox enzymes such as oxidases and protein disulfide isomerases (PDIs). [27][28][29] PDIs are multi-domain proteins. As common feature they share at least one trx- 119,120 and TRX-3 is only present in the intestine.…”

Section: Protein Disulfide Isomerases -Pdi'smentioning

confidence: 99%

Interplay between redox and protein homeostasis

Feleciano

Arnsburg

Kirstein

2016

Worm

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The subcellular compartments of eukaryotic cells are characterized by different redox environments. Whereas the cytosol, nucleus and mitochondria are more reducing, the endoplasmic reticulum represents a more oxidizing environment. As the redox level controls the formation of intra-and inter-molecular disulfide bonds, the folding of proteins is tightly linked to its environment. The proteostasis network of each compartment needs to be adapted to the compartmental redox properties. In addition to chaperones, also members of the thioredoxin superfamily can influence the folding of proteins by regulation of cysteine reduction/oxidation. This review will focus on thioredoxin superfamily members and chaperones of C. elegans, which play an important role at the interface between redox and protein homeostasis. Additionally, this review will highlight recent methodological developments on in vivo and in vitro assessment of the redox state and their application to provide insights into the high complexity of redox and proteostasis networks of C. elegans.

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Functional in Vitro Analysis of the ERO1 Protein and Protein-disulfide Isomerase Pathway

Cited by 76 publications

References 37 publications

Anticancer effect of icaritin inhibits cell growth of colon cancer through reactive oxygen species, Bcl-2 and cyclin D1/E signaling

Anticancer effect of icaritin inhibits cell growth of colon cancer through reactive oxygen species, Bcl-2 and cyclin D1/E signaling

Hyperactivity of the Ero1α Oxidase Elicits Endoplasmic Reticulum Stress but No Broad Antioxidant Response

Interplay between redox and protein homeostasis

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