Functional inactivation rates of the messenger RNA molecules coding for the individual ribosomal proteins of Eschrichia coli

Barnsley, Patricia G. H.; Sells, Bruce H.

doi:10.1007/bf00264726

Cited by 6 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7 and 8) as suggested previously in the soluble proteins of C. crescentus CB15 (3). In contrast, the functional half-life of mRNA for each major protein estimated from the residual rate of synthesis of the protein after inhibition of RNA synthesis was found to be short (0.4 to 5.8 min), as also reported in E. coli (1). The functional half-life for the total mRNA in C. crescentus CB13 was estimated to be 1.5 to 2.2 min, which is similar to the chemical half-life of the bulk mRNA from C. crescentus CB15 (13).…”

Section: Resultssupporting

confidence: 70%

Rate of major protein synthesis during the cell cycle of Caulobacter crescentus

Iba¹,

Fukuda²,

Okada³

1978

J Bacteriol

View full text Add to dashboard Cite

The rate of major protein synthesis was examined during the synchronous differentiation of Caulobacter crescentus. Total cell proteins were pulse-labeled with [35S]methionine at different times in the swarmer cell cycle and analyzed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis. The rates of synthesis of total cell proteins and of about one-half of the individual major proteins examined increased through G1 and S periods but remained nearly constant during G2 period. The rates of synthesis of the other half of the individual major proteins either increased continuously throughout the swarmer cell cycle or doubled during S period. One stage-specific protein was also detected in late S period. For most of the major proteins examined, the rate of synthesis in the swarmer cell was less than that in the stalked cell. It seemed that, before the onset of G2 period, the Caulobacter cell was already able to synthesize each major protein at the additive rate of the two progeny cells. Compared to the stability of cellular proteins, the functional degradation rate of mRNA coding for individual major proteins was rapid, with half-lives of 0.4 to 5.8 min. It thus seems that the rate of major protein synthesis mainly reflects the transcriptional control of gene expression.

show abstract

Section: Resultssupporting

confidence: 70%

Rate of major protein synthesis during the cell cycle of Caulobacter crescentus

Iba¹,

Fukuda²,

Okada³

1978

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Our results extend this to the P22 late operon. Measurements of the functional half-life of bulk enterobacterial mRNA range from 1.1 to 3.5 min (1,10,17), similar to the rates of inactivation of the P22 late operon transcripts. Since it takes an RNA polymerase 7 to 10 min to transcribe the late operon and the half-lives of at least four of the late gene transcripts are less than 2.6 min, it is unlikely that an appreciable amount of full-length late operon message is ever present in the infected cell.…”

Section: Vol 53 1985mentioning

confidence: 81%

Posttranscriptional modulation of bacteriophage P22 scaffolding protein gene expression

Casjens¹,

Adams²

1985

J Virol

View full text Add to dashboard Cite

The bacteriophage P22 late operon contains 2 genes whose products are required for cell lysis and 13 genes whose products are involved in the morphogenesis of the phage particle. This operon is under the positive control of the phage gene 23 product and is thought to have a single promoter. The expression of one of these late genes, the scaffolding protein gene, is autogenously modulated independently from the remainder of the late genes. When unassembled, scaffolding protein turns down the rate of synthesis of additional scaffolding protein, and when it is assembled into phage precursor structures, it does not. Experiments presented here show (i) that the mRNA from the scaffolding protein gene is functionally threefold more stable when most of the scaffolding protein is assembled than when it is unassembled and (ii) that no new promoter near the scaffolding protein gene is activated at the high level of synthesis. These data support the model that this autogenous modulation occurs at a posttranscriptional level. We also observed that another message, that of coat protein, appears to become increasingly stable with time after phage infection.

show abstract

Synthesis of messenger RNA coding for individual ribosomal proteins during nutritional shift-up in Escherichia coli K-12

Barnsley

Sells

1978

Molec. Gen. Genet.

View full text Add to dashboard Cite

Functional inactivation rates of the messenger RNA molecules coding for the individual ribosomal proteins of Eschrichia coli

Cited by 6 publications

References 13 publications

Rate of major protein synthesis during the cell cycle of Caulobacter crescentus

Rate of major protein synthesis during the cell cycle of Caulobacter crescentus

Posttranscriptional modulation of bacteriophage P22 scaffolding protein gene expression

Synthesis of messenger RNA coding for individual ribosomal proteins during nutritional shift-up in Escherichia coli K-12

Contact Info

Product

Resources

About