Functional Kidney Bioengineering with Pluripotent Stem‐Cell‐Derived Renal Progenitor Cells and Decellularized Kidney Scaffolds

Du, Chan; Narayanan, Karthikeyan; Leong, Meng Fatt; Ibrahim, M. S.; Chua, Ying Ping; Khoo, Vanessa Mei Hui; Wan, Andrew C.A.

doi:10.1002/adhm.201600120

Cited by 57 publications

(45 citation statements)

References 37 publications

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“…Nevertheless, currently recellularized renal and liver scaffolds can be used for the development of cellular assays for drug screening conditions. [245]…”

Section: Outlook and Perspectives Of Organ Decellularization/ Recellumentioning

confidence: 99%

Bioengineering Organs for Blood Detoxification

Legallais

Kim

Mihaila

et al. 2018

Adv Healthcare Materials

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For patients with severe kidney or liver failure the best solution is currently organ transplantation. However, not all patients are eligible for transplantation and due to limited organ availability, most patients are currently treated with therapies using artificial kidney and artificial liver devices. These therapies, despite their relative success in preserving the patients' life, have important limitations since they can only replace part of the natural kidney or liver functions. As blood detoxification (and other functions) in these highly perfused organs is achieved by specialized cells, it seems relevant to review the approaches leading to bioengineered organs fulfilling most of the native organ functions. There, the culture of cells of specific phenotypes on adapted scaffolds that can be perfused takes place. In this review paper, first the functions of kidney and liver organs are briefly described. Then artificial kidney/liver devices, bioartificial kidney devices, and bioartificial liver devices are focused on, as well as biohybrid constructs obtained by decellularization and recellularization of animal organs. For all organs, a thorough overview of the literature is given and the perspectives for their application in the clinic are discussed.

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“…Nevertheless, currently recellularized renal and liver scaffolds can be used for the development of cellular assays for drug screening conditions. [245]…”

Section: Outlook and Perspectives Of Organ Decellularization/ Recellumentioning

confidence: 99%

Bioengineering Organs for Blood Detoxification

Legallais

Kim

Mihaila

et al. 2018

Adv Healthcare Materials

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“…To avoid the in vivo differentiation process of PSCs, which could be partial, in 2016 Du et al injected into mouse decellularized kidney Pax2+ renal progenitor cells and endothelial cells derived from human iPSCs [82]. Following implantation for 12 weeks of the recellularized kidney into immunodeficient mice, the authors observed effective repopulation of the glomeruli only in the presence of both Pax2+ and endothelial cells concluding that endothelial cells are required for cellular assembly of the glomerular structures while they do not affect tubule repopulation [82]. In addition, the presence of endothelial cells positively influenced the filtration potential of the glomerular units measured by employing a bioreactor in vitro.…”

Section: Kidney-derived Scaffoldsmentioning

confidence: 99%

Bioengineering strategies for nephrologists: kidney was not built in a day

Peired

Mazzinghi

Chiara

et al. 2020

Expert Opinion on Biological Therapy

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Introduction: The number of patients with end-stage kidney disease is increasing worldwide, creating an unprecedented organ shortage. The kidney is a highly complex structure performing many crucial functions. Dialysis replaces filtration but not all other kidney functions and transplant is limited by kidney availability. Numerous innovative ways are being explored to obtain new kidneys for disease modeling and potentially replace lost kidney functions. Areas covered: In this review, we will go through the different approaches that have been developed over the years to build kidneys. We will first present the current advances in xenotransplantation and generation of interspecies chimeras. Next, we will examine the attempts to create bioengineered kidneys with hemodialysis-derived implantable devices and decellularized organs. Finally, we will examine how organoids and microfluidic devices could answer important pathophysiological questions and model the path toward creating in vitro functional organs, for example through 3D bioprinting. Expert opinion: While all the aforementioned approaches to create new kidneys are promising, their translation into clinical practice seems a long way off, except xenotransplantation. Nonetheless, these novel technologies already consent disease modeling and drug testing at 3D level. We will review the stages of progress toward patient therapy and advantages/drawbacks of the various strategies.

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“…Results showed that only in the presence of endothelial cells were the glomeruli recellularized. Moreover, they showed in-vitro how endothelial cells improved glomerular barrier function (Du et al, 2016 ). Very recently Bombelli et al derived human nephrospheres, the spheres were shown to contain renal stem cell like cells.…”

Section: Kidneymentioning

confidence: 99%

Whole Organ Tissue Vascularization: Engineering the Tree to Develop the Fruits

Pellegata

Tedeschi

Coppi

2018

Front. Bioeng. Biotechnol.

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Tissue engineering aims to regenerate and recapitulate a tissue or organ that has lost its function. So far successful clinical translation has been limited to hollow organs in which rudimental vascularization can be achieved by inserting the graft into flaps of the omentum or muscle fascia. This technique used to stimulate vascularization of the graft takes advantage of angiogenesis from existing vascular networks. Vascularization of the engineered graft is a fundamental requirement in the process of engineering more complex organs, as it is crucial for the efficient delivery of nutrients and oxygen following in-vivo implantation. To achieve vascularization of the organ many different techniques have been investigated and exploited. The most promising results have been obtained by seeding endothelial cells directly into decellularized scaffolds, taking advantage of the channels remaining from the pre-existing vascular network. Currently, the main hurdle we need to overcome is achieving a fully functional vascular endothelium, stable over a long time period of time, which is engineered using a cell source that is clinically suitable and can generate, in vitro, a yield of cells suitable for the engineering of human sized organs. This review will give an overview of the approaches that have recently been investigated to address the issue of vascularization in the field of tissue engineering of whole organs, and will highlight the current caveats and hurdles that should be addressed in the future.

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Functional Kidney Bioengineering with Pluripotent Stem‐Cell‐Derived Renal Progenitor Cells and Decellularized Kidney Scaffolds

Cited by 57 publications

References 37 publications

Bioengineering Organs for Blood Detoxification

Bioengineering Organs for Blood Detoxification

Bioengineering strategies for nephrologists: kidney was not built in a day

Whole Organ Tissue Vascularization: Engineering the Tree to Develop the Fruits

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