Functional Metabolomics Reveals that Astragalus Polysaccharides Improve Lipids Metabolism through Microbial Metabolite 2-Hydroxybutyric Acid in Obese Mice

Li, Bingbing; Hong, Ying; Gu, Yu; Ye, Shengjie; Hu, Kaili; Yao, Juanjuan; Ding, Kan; Zhao, Aihua; Wang, Jia; Li, Houkai

doi:10.1016/j.eng.2020.05.023

Cited by 19 publications

(9 citation statements)

References 77 publications

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“…The current study found that APS had a therapeutic effect on acute colitis induced by sodium dextran sulfate and could restore the structure of gut microbiota 31 . They could also increase the level of 2‐hydroxybutyric acid in the serum and liver of mice by regulating gut microbiota and improve lipid metabolism disorders in vivo and in vitro 32 . Therefore, we investigated whether the effect of the antitumor immunity of APS depended on the gut microbiota remodeling.…”

Section: Discussionmentioning

confidence: 91%

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Immunosuppressive activity is attenuated by Astragalus polysaccharides through remodeling the gut microenvironment in melanoma mice

Ding

Gong

et al. 2021

Cancer Science

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Section: Discussionmentioning

confidence: 91%

“…31 They could also increase the level of 2-hydroxybutyric acid in the serum and liver of mice by regulating gut microbiota and improve lipid metabolism disorders in vivo and in vitro. 32 Therefore, we investigated whether the effect of the antitumor immunity of APS depended on the gut microbiota remodeling.…”

Section: Discussionmentioning

confidence: 99%

Immunosuppressive activity is attenuated by Astragalus polysaccharides through remodeling the gut microenvironment in melanoma mice

Ding

Gong

et al. 2021

Cancer Science

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“…Polysaccharides supplementation has been reported to alter the level of metabolites related to lipid metabolism including oleic acid and docosahexaenoic acid (65). These fatty acids could be synthesized by intestinal microbiota such as Lactobacillus species and Eubacterium ventriosum from alpha-linoleic acid as a substrate (66).…”

Section: Figure 13mentioning

confidence: 99%

Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens

et al. 2022

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The intestinal microbiome is responsible for the fermentation of complex carbohydrates and orchestrates the immune system through gut microbiota-derived metabolites. In our previous study, we reported that supplementation of Enteromorpha polysaccharide (EP) and yeast glycoprotein (YG) in combination synergistically improved antioxidant activities, serum lipid profile, and fatty acid metabolism in chicken. However, the mechanism of action of these polysaccharides remains elusive. The present study used an integrated 16S-rRNA sequencing technology and untargeted metabolomics technique to reveal the mechanism of action of EP+YG supplementation in broiler chickens fed basal diet or diets supplemented with EP+YG (200mg/kg EP + 200mg/kg YG). The results showed that EP+YG supplementation altered the overall structure of caecal microbiota as evidenced by β diversities analysis. Besides, EP+YG supplementation changed the microbiota composition by altering the community profile at the phylum and genus levels. Furthermore, Spearman correlation analysis indicated a significant correlation between altered microbiota genera vs serum cytokine levels and microbiota genera vs volatile fatty acids production. Predicted functional analysis showed that EP+YG supplementation significantly enriched amino acid metabolism, nucleotide metabolism, glycan biosynthesis and metabolism, energy metabolism, and carbohydrate metabolism. Metabolomics analysis confirmed that EP+YG supplementation modulates a myriad of caecal metabolites by increasing some metabolites, including pyruvic acid, pyridoxine, spermidine, spermine, and dopamine, and decreasing metabolites related to lipid metabolisms such as malonic acid, oleic acid, and docosahexaenoic acid. The quantitative enrichment analysis results further showed that glycolysis/gluconeogenesis, citric acid cycle, tyrosine metabolism, glycine, serine, and threonine metabolism, and cysteine and methionine metabolism were the most important enriched pathways identified with enrichment ratio >11, whereas, fatty acid biosynthesis and biosynthesis of unsaturated fatty acids pathways were suppressed. Together, the 16S-rRNA and untargeted metabolomics results uncovered that EP+YG supplementation modulates intestinal microbiota and their metabolites, thereby influencing the important metabolism pathways, suggesting a potential feed additive.

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“…[ 41 ] In addition, α‐hydroxybutyrate, converted from 2‐ketobutyrate in adipocyte, modulates lipid metabolism both in vitro and in vivo. [ 42,43 ] Therefore, our global metabolomic analysis suggests that 2‐ketobutyrate should mediate the bacterial anti‐obesity effects.…”

Section: Resultsmentioning

confidence: 99%

Bifidobacterium lactis IDCC 4301 Exerts Anti‐Obesity Effects in High‐Fat Diet‐Fed Mice Model by Regulating Lipid Metabolism

Ban

Lee

Bang

et al. 2022

Molecular Nutrition Food Res

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Scope Chronic hypernutrition promotes lipid accumulation in the body and excessive lipid accumulation leads to obesity. An increase in the number and size of adipocytes, a characteristic of obesity is closely associated with adipose dysfunction. Recent in vitro and in vivo studies have shown that probiotics may prevent this dysfunction by regulating lipid metabolism. However, the mechanisms of action of probiotics in obesity are not fully understood and their usage for treating obesity remains limited. Methods and results Bifidobacterium lactis IDCC 4301 is selected for its anti‐obesity potential after evaluating inhibitory activity of pancreatic lipase and cholesterol reducing activity. Next, this study investigates the roles of B. lactis IDCC 4301 on lipid metabolism in 3T3‐L1 preadipocytes and high‐fat diet (HFD)‐fed mice. B. lactis IDCC 4301 inhibits cell differentiation and lipid accumulation by suppressing the expression of adipogenic enzymes in 3T3‐L1 cells. Moreover, the administration of B. lactis IDCC 4301 decreases body and adipose tissue weight, improves serum lipid levels, and downregulates adipogenic mRNA expression in HFD‐fed mice. Additionally, metabolomic analysis suggests that 2‐ketobutyrate should be a possible target compound against obesity. Conclusions B. lactis IDCC 4301 may be used as an alternative treatment for obesity.

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Functional Metabolomics Reveals that Astragalus Polysaccharides Improve Lipids Metabolism through Microbial Metabolite 2-Hydroxybutyric Acid in Obese Mice

Cited by 19 publications

References 77 publications

Immunosuppressive activity is attenuated by Astragalus polysaccharides through remodeling the gut microenvironment in melanoma mice

Immunosuppressive activity is attenuated by Astragalus polysaccharides through remodeling the gut microenvironment in melanoma mice

Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens

Bifidobacterium lactis IDCC 4301 Exerts Anti‐Obesity Effects in High‐Fat Diet‐Fed Mice Model by Regulating Lipid Metabolism

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