Functional microsatellite and possible selective sweep in natural populations of the black-chinned tilapia Sarotherodon melanotheron (Teleostei, Cichlidae)

Agnèse, Jean‐François; Adépo-Gourène, Béatrice; Nyingi, Dorothy Wanja

doi:10.1016/j.margen.2008.10.004

Cited by 12 publications

(10 citation statements)

References 22 publications

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“…Following this observation, a ‘short’ orthologous PRL allele was found fixed by local selection in a freshwater population of another euryhaline species of tilapia ( Sarotherodon melanotheron ), while marine or brackish populations were polymorphic at this locus (Agnèse et al . ). In the flathead grey mullet ( Mugil cephalus ), Blel et al .…”

Section: Discussionmentioning

confidence: 97%

Microsatellite length variation in candidate genes correlates with habitat in the gilthead sea bream Sparus aurata

et al. 2012

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The genetic basis and evolutionary implications of local adaptation in high gene flow marine organisms are still poorly understood. In several Mediterranean fish species, alternative migration patterns exist between individuals entering coastal lagoons that offer favourable conditions for growth and those staying in the sea where environmental conditions are less subject to rapid and stressful change. Whether these coexisting strategies are phenotypically plastic or include a role for local adaptation through differential survival needs to be determined. Here, we explore the genetic basis of alternate habitat use in western Mediterranean populations of the gilthead sea bream (Sparus aurata). Samples from lagoonal and open-sea habitats were typed for three candidate gene microsatellite loci, seven anonymous microsatellites and 44 amplified fragment length polymorphism markers to test for genotype-environment associations. While anonymous markers globally indicated high levels of gene flow across geographic locations and habitats, non-neutral differentiation patterns correlated with habitat type were found at two candidate microsatellite loci located in the promoter region of the growth hormone and prolactin genes. Further analysis of these two genes revealed that a mechanism based on habitat choice alone could not explain the distribution of genotype frequencies at a regional scale, thus implying a role for differential survival between habitats. We also found an association between allele size and habitat type, which, in the light of previous studies, suggests that polymorphisms in the proximal promoter region could influence gene expression by modulating transcription factor binding, thus providing a potential explanatory link between genotype and growth phenotype in nature.

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Section: Discussionmentioning

confidence: 97%

Microsatellite length variation in candidate genes correlates with habitat in the gilthead sea bream Sparus aurata

et al. 2012

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“…positive F is values) at population level in P. taeniatus from Moliwe and P. pulcher from Nigeria, which might indicate non-random mating. Heterozygote deficiencies revealed in several studies indicate that inbreeding is quite common in fish [81] , [83] , [85] – [87] . The process underlying kin mating, however, cannot be directly inferred from population genetic data.…”

Section: Discussionmentioning

confidence: 99%

Microsatellite Support for Active Inbreeding in a Cichlid Fish

et al. 2011

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In wild animal populations, the degree of inbreeding differs between species and within species between populations. Because mating with kin often results in inbreeding depression, observed inbreeding is usually regarded to be caused by limited outbreeding opportunities due to demographic factors like small population size or population substructuring. However, theory predicts inclusive benefits from mating with kin, and thus part of the observed variation in inbreeding might be due to active inbreeding preferences. Although some recent studies indeed report kin mating preferences, the evidence is still highly ambiguous. Here, we investigate inbreeding in a natural population of the West African cichlid fish Pelvicachromis taeniatus which showed clear kin mating preferences in standardized laboratory experiments but no inbreeding depression. The presented microsatellite analysis reveals that the natural population has, in comparison to two reference populations, a reduced allelic diversity (A = 3) resulting in a low heterozygosity (Ho = 0.167) pointing to a highly inbred population. Furthermore, we found a significant heterozygote deficit not only at population (Fis = 0.116) but also at subpopulation level (Fis = 0.081) suggesting that inbreeding is not only a by-product of population substructuring but possibly a consequence of behavioral kin preferences.

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“…A total of 16 microsatellite loci were analysed in this study: PRL1AC and PRL1GT [20] , UNH104 (GenBank reference number G12257), UNH115 (G12268), UNH129 (G12282), UNH142 (G12294), UNH146 (G12298), UNH154 (G12306), UNH162 (G12314), UNH189 (G12341), UNH211 (G12362), UNH860 (G68195), UNH874 (G68202), UNH887 (G68210), UNH995 (G68274) and UNH1003 (G68280). Amplifications were carried out using Qiagen Multiplex PCR Kit and following manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

An Important Natural Genetic Resource of Oreochromis niloticus (Linnaeus, 1758) Threatened by Aquaculture Activities in Loboi Drainage, Kenya

2014

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The need to improve food security in Africa through culture of tilapias has led to transfer of different species from their natural ranges causing negative impacts on wild fish genetic resources. Loboi swamp in Kenya is fed by three hot springs: Lake Bogoria Hotel, Chelaba and Turtle Springs, hosting natural populations of Oreochromis niloticus. The present study aimed at better genetic characterization of these threatened populations. Partial mtDNA sequences of the D-loop region and variations at 16 microsatellite loci were assessed in the three hot spring populations and compared with three other natural populations of O. niloticus in the region. Results obtained indicated that the hot spring populations had mitochondrial and nuclear genetic variability similar to or higher than the large closely related populations. This may be attributed to the perennial nature of the hot springs, which do not depend on rainfall but rather receive permanent water supply from deep aquifers. The study also revealed that gene flow between the three different hot spring populations was sufficiently low thus allowing their differentiation. This differentiation was unexpected considering the very close proximity of the springs to each other. It is possible that the swamp creates a barrier to free movement of fish from one spring to the other thereby diminishing gene flow. Finally, the most surprising and worrying results were that the three hot spring populations are introgressed by mtDNA genes of O. leucostictus, while microsatellite analysis suggested that some nuclear genes may also have crossed the species barrier. It is very likely that the recent intensification of aquaculture activities in the Loboi drainage may be responsible for these introgressions. Taking into account the importance of these new genetic resources, protection and management actions of the Loboi swamp should be accorded top priority to prevent the loss of these spring populations.

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Functional microsatellite and possible selective sweep in natural populations of the black-chinned tilapia Sarotherodon melanotheron (Teleostei, Cichlidae)

Cited by 12 publications

References 22 publications

Microsatellite length variation in candidate genes correlates with habitat in the gilthead sea bream Sparus aurata

Microsatellite length variation in candidate genes correlates with habitat in the gilthead sea bream Sparus aurata

Microsatellite Support for Active Inbreeding in a Cichlid Fish

An Important Natural Genetic Resource of Oreochromis niloticus (Linnaeus, 1758) Threatened by Aquaculture Activities in Loboi Drainage, Kenya

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