Functional multispectral optoacoustic tomography imaging of hepatic steatosis development in mice

Huang, Shan; Blutke, Andreas; Feuchtinger, Annette; Klemm, Uwe; Tom, Robby Zachariah; Hofmann, Susanna M.; Stiel, André C.; Ntziachristos, Vasilis

doi:10.15252/emmm.202013490

Cited by 16 publications

(25 citation statements)

References 46 publications

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“…Contrast agents may also be used for an enhanced TROPUS performance. For example, indocyanine green (ICG) is mainly cleared through the liver, and thus can be used to assess functional differences between healthy and NAFLD mice by comparing clearance time [22]. Different types of nanoparticles can also be used to boost imaging sensitivity and contrast [46]- [49].…”

Section: Discussionmentioning

confidence: 99%

“…It is based on optical excitation of tissues at near-infrared (NIR) wavelengths and tomographic detection of the thermoelastically-induced US waves, thus rendering rich optical contrast with high spatial resolution unaffected by photon scattering in deep tissues [19], [20]. In particular, multispectral optoacoustic tomography (MSOT) capitalizes on optical excitation at different wavelengths to spectroscopically differentiate between oxy- (HbO 2 ) and deoxy-hemoglobin (Hb), melanin, lipids and other tissue bio-chromes as well as extrinsically administered contrast agents [21],[22]. However, unambiguous anatomical differentiation of lesions and organs is hindered with MSOT whose main contrast stems from hemoglobin-rich structures such as major blood vessels.…”

Section: Introductionmentioning

confidence: 99%

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Multimodal Assessment of Non-Alcoholic Fatty Liver Disease with Transmission-Reflection Optoacoustic Ultrasound

Lafci

Hadjihambi

Konstantinou

et al. 2022

Preprint

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Non-alcoholic fatty liver disease (NAFLD) is an umbrella term referring to a group of conditions associated to fat deposition and damage of liver tissue. Early detection of fat accumulation is essential to avoid progression of NAFLD to serious pathological stages such as liver cirrhosis and hepatocellular carcinoma. We exploited the unique capabilities of transmission-reflection optoacoustic ultrasound (TROPUS), which combines the advantages of optical and acoustic contrasts, for an early-stage multi-parametric assessment of NAFLD in mice. The multispectral optoacoustic imaging allowed for spectroscopic differentiation of lipid content, as well as the bio-distributions of oxygenated and deoxygenated hemoglobin in liver tissues in vivo. The pulse-echo (reflection) ultrasound (US) imaging further provided a valuable anatomical reference whilst transmission US facilitated the mapping of speed of sound changes in lipid-rich regions, which was consistent with the presence of macrovesicular hepatic steatosis in the NAFLD livers examined with ex vivo histological staining. The proposed multimodal approach facilitates quantification of liver abnormalities at early stages using a variety of optical and acoustic contrasts, laying the ground for translating the TROPUS approach toward diagnosis and monitoring NAFLD in patients.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multimodal Assessment of Non-Alcoholic Fatty Liver Disease with Transmission-Reflection Optoacoustic Ultrasound

Lafci

Hadjihambi

Konstantinou

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…According to the perpendicular or parallel relationship between incident light and the signal receiving surface of the detector, PACT systems can be roughly classified into two types [6][7][8]11,22,35 (Figure 1). The transducer of the former (Type I) is usually rectangular or arc-shaped, volumetric images are obtained by rotating the transducers or rotating the imaging object 8,11,20 (Figure 1a). The transducer of the latter (Type II) is usually ring-shaped to obtain cross-sectional images.…”

Section: Pact Systems Applied In Biomedical Imagingmentioning

confidence: 99%

“…19 In 2021, Huang et al used multispectral optoacoustic tomography (MSOT) to visualize lipids in laboratory mice, and demonstrated that MSOT was an efficient imaging technique for lipid visualization in the preclinical model of fatty liver disease. 20 In 2022, Vagenknecht et al demonstrated tau protein imaging in mice brain using tau-targeted pyridinyl-butadienyl-benzothiazole derivative PBB5 and MSOT. 21 Clinical research of PACT mainly focuses on the examination of human breast cancer and skin melanoma.…”

mentioning

confidence: 99%

Application of photoacoustic computed tomography in biomedical imaging: A literature review

Sun

Wang

et al. 2022

Bioengineering & Transla Med

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Photoacoustic computed tomography (PACT) is a hybrid imaging modality that combines optical excitation and acoustic detection techniques. It obtains high‐resolution deep‐tissue images based on the deep penetration of light, the anisotropy of light absorption in objects, and the photoacoustic effect. Hence, PACT shows great potential in biomedical sample imaging. Recently, due to its advantages of high sensitivity to optical absorption and wide scalability of spatial resolution with the desired imaging depth, PACT has received increasing attention in preclinical and clinical practice. To date, there has been a proliferation of PACT systems designed for specific biomedical imaging applications, from small animals to human organs, from ex vivo to in vivo real‐time imaging, and from simple structural imaging to functional and molecular imaging with external contrast agents. Therefore, it is of great importance to summarize the previous applications of PACT systems in biomedical imaging and clinical practice. In this review, we searched for studies related to PACT imaging of biomedical tissues and samples over the past two decades; divided the studies into two categories, PACT imaging of preclinical animals and PACT imaging of human organs and body parts; and discussed the significance of the studies. Finally, we pointed out the future directions of PACT in biomedical applications. With the development of exogenous contrast agents and advances of imaging technique, in the future, PACT will enable biomedical imaging from organs to whole bodies, from superficial vasculature to internal organs, from anatomy to functions, and will play an increasingly important role in biomedical research and clinical practice.

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“…Over the last years, PAI has shown great potential towards bioimaging applications, including the visualization of diseased organs, such as thyroid [187], liver [188], and skin [189], or the real-time monitoring of cancerous cells [190,191]. However, despite its exponential growth, its clinical translation is still limited due to certain factors, which are highlighted below along with proposed solutions.…”

Section: Limitations and Future Perspectivementioning

confidence: 99%

Design Principles Governing the Development of Theranostic Anticancer Agents and Their Nanoformulations with Photoacoustic Properties

et al. 2022

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The unmet need to develop novel approaches for cancer diagnosis and treatment has led to the evolution of theranostic agents, which usually include, in addition to the anticancer drug, an imaging agent based mostly on fluorescent agents. Over the past few years, a non-invasive photoacoustic imaging modality has been effectively integrated into theranostic agents. Herein, we shed light on the design principles governing the development of theranostic agents with photoacoustic properties, which can be formulated into nanocarriers to enhance their potency. Specifically, we provide an extensive analysis of their individual constituents including the imaging dyes, drugs, linkers, targeting moieties, and their formulation into nanocarriers. Along these lines, we present numerous relevant paradigms. Finally, we discuss the clinical relevance of the specific strategy, as also the limitations and future perspectives, and through this review, we envisage paving the way for the development of theranostic agents endowed with photoacoustic properties as effective anticancer medicines.

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Functional multispectral optoacoustic tomography imaging of hepatic steatosis development in mice

Cited by 16 publications

References 46 publications

Multimodal Assessment of Non-Alcoholic Fatty Liver Disease with Transmission-Reflection Optoacoustic Ultrasound

Multimodal Assessment of Non-Alcoholic Fatty Liver Disease with Transmission-Reflection Optoacoustic Ultrasound

Application of photoacoustic computed tomography in biomedical imaging: A literature review

Design Principles Governing the Development of Theranostic Anticancer Agents and Their Nanoformulations with Photoacoustic Properties

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