Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells

Dong, Yi; Hsu, Fu‐Chun; Koziol‐White, Cynthia; Stepanova, Victoria; Jude, Joseph; Gritsiuta, Andrei I.; Rue, Ryan W.; Mott, Rosalind; Coulter, Douglas A.; Panettieri, Reynold A.; Krymskaya, Vera P.; Takano, Hajime; Goncharova, Elena A.; Goncharov, Dmitry A.; Cines, Douglas B.; Lynch, David R.

doi:10.1038/s41598-021-87667-0

Cited by 16 publications

(14 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 4 GluN1 and all four GluN2 subunits were also expressed in the human pulmonary artery smooth muscle cells. 22 Overall, these findings indicate that NMDA receptors could control the respiratory tract function in vertebrate animals. 23 …”

Section: Expression Of Nmda Receptors In the Human Lungsmentioning

confidence: 85%

NMDA receptor modulation and severe acute respiratory syndrome treatment

Costa

2021

F1000Res

View full text Add to dashboard Cite

N-Methyl-D-aspartate (NMDA) subtype of glutamate receptors is expressed in the human lungs and central nervous system. NMDA receptor potentiation could increase calcium ion influx and promote downstream signaling mechanisms associated with cellular contractions that are disrupted in severe acute respiratory syndrome. Pharmacological effects generated by triggering glutamate receptor function in the brain, coupled with concurrent stimulation of the respiratory tract, may produce a synergetic effect, improving the airway smooth muscle function. A novel multipronged intervention to simultaneously potentiate NMDA receptors expressed both in the central nervous system and airway muscles would be helpful for the treatment of severe acute respiratory syndrome that deteriorates peripheral and central nervous system function before causing death in humans.

show abstract

Section: Expression Of Nmda Receptors In the Human Lungsmentioning

confidence: 85%

NMDA receptor modulation and severe acute respiratory syndrome treatment

Costa

2021

F1000Res

View full text Add to dashboard Cite

show abstract

“…Studies performed on rodent models and cell lines have shown that the predominant site of action for ketamine is neurons due to expression pattern of ketamine receptors. However, recent studies demonstrated extraneuronal sites for ketamine 51 . Our findings suggest that ketamine is likely to act nonautonomously to induce vulval and cuticular changes.…”

Section: Discussionmentioning

confidence: 99%

Ketamine induces apical extracellular matrix modifications in Caenorhabditis elegans

Yücel

2022

Sci Rep

View full text Add to dashboard Cite

Ketamine is a widely used anesthetic agent since 1960s and has recently been exploited for its rapid antidepressant action at subanesthetic doses. It has been demonstrated that ketamine induces alterations in extracellular matrix (ECM) in rodent models which in part plays a role in its anti-depressant action. The nematode Caenorhabditis elegans serves as a powerful tool for understanding mechanisms of drug action with its short life cycle, genetic amenability and conserved cellular processes. Further investigation is required particularly in in vivo systems to gain broader understanding of ketamine’s actions. In this study, we aimed to decipher ketamine-mediated alterations using C. elegans as a model. We show that ketamine specifically induces apical extracellular matrix modifications (aECM) in the vulva and the cuticle. Ketamine treatment phenocopies neuronal migration and vulval invagination defects of chondroitin mutants despite wild-type like chondroitin staining pattern. Normal vulval expansion and defective vulval eversion phenotypes of ketamine-treated animals are suggestive of alterations in the network of aECM factors which do not impinge on chondroitin. Ketamine ameliorates impaired movement of a group of roller mutants characterised with collagen defects in the cuticle and RNA-seq identifies that 30% of the cuticular collagens are upregulated in response to ketamine. Ketamine alters aECM, neuronal migration and collagen expression in C. elegans. We propose C. elegans as a putative animal model to investigate ketamine-mediated ECM modifications.

show abstract

“…These cells have been reported to cause constrictions in the airway by activating the NMDA receptor. [24,25] ARS has an antagonistic effect on NMDA receptors. It has also been reported that the blockade of the NMDA receptor prevents oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…NMDA receptors are known for their function in neurons and their role in the pathophysiology of epilepsy. [13,24] However, studies on its presence in the lung and other tissues are limited. A recent study identified lung smooth muscle cell types that express NMDA receptors.…”

Section: Discussionmentioning

confidence: 99%

Deneysel Epileptik Nöbet Modelinde, Artemisinin'in Fare Kalp ve Akciğer Dokularında Oksidatif Stress Belirteçleri Üzerine Etkisi.

KOÇAK

Huyut

Türkan

et al. 2022

Journal of Contemporary Medicine

View full text Add to dashboard Cite

Aim: The current study investigated the effects of artemisinin on the heart and lung tissue against pentylenetetrazol-induced seizures in mice. For this purpose, malondialdehyde (MDA), advanced oxidation protein products (AOPP), Catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels were evaluated in both tissue homogenates.Material and Method: Swiss albino male mice (n=42) were used in the experiment. Animals were divided into six groups; Control (C), pentylenetetrazol (PTZ), valproate 100 mg/kg (VPA), artemisinin 30 mg/kg (ARS)+PTZ, ARS 60 mg/kg+PTZ, ARS 120 mg/kg+PTZ. On the 26th day of the experiment, the mice were sacrificed and the samples were kept at -80 0C for biochemical analysis.Results: There were significant differences in the five biochemical parameters analyzed in heart and lung tissues. Heart and lung MDA levels of the PTZ group were found to be significantly higher than the C and ARS-60 groups (p<0.05). Heart and lung MDA levels of the PTZ group were found to be significantly higher than the C and ARS-60 groups. Likewise, heart AOPP levels decreased significantly in the VPA and ARS-60 groups compared to the PTZ group (p<0.05). There was no significant difference between the groups in terms of lung AOPP levels (p>0.05). Heart CAT and GSH levels were decreased in the PTZ group compared to the other groups. However, in terms of Lung CAT levels, the PTZ group had the highest value compared to the other groups, while it had the lowest value in terms of GSH level. The GSH-Px level did not differ significantly between the groups in heart tissue (p>0.05). The lung GSH-Px level was significantly increased in the ARS-30 group when compared to the PTZ group (p<0.05).Conclusion: Consequently ARS treatment can inhibit PTZ-induced oxidative stress in peripheral tissues. In addition, ARS may provide improvements in decreased antioxidant enzymes. ARS may contribute to the antioxidant defense system.

show abstract

Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells

Cited by 16 publications

References 50 publications

NMDA receptor modulation and severe acute respiratory syndrome treatment

NMDA receptor modulation and severe acute respiratory syndrome treatment

Ketamine induces apical extracellular matrix modifications in Caenorhabditis elegans

Deneysel Epileptik Nöbet Modelinde, Artemisinin'in Fare Kalp ve Akciğer Dokularında Oksidatif Stress Belirteçleri Üzerine Etkisi.

Contact Info

Product

Resources

About