Functional Properties of Mesenchymal Stem Cells Labeled with Magnetic Microparticles in Vitro and Analysis of Their Distribution after Transplantation

Konieva, A. A.; Холоденко, И. В.; Shragina, Olga; Холоденко, Р. В.; Burunova, V. V.; Bibaeva, L. V.; Yarygin, K. N.; Yarygin, V. N.

doi:10.1007/s10517-010-1087-9

Cited by 7 publications

(3 citation statements)

References 9 publications

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“…Earlier we have shown that labeled hMSCs retained the SPIO microparticles for at least four weeks [ 33 ]. Moreover, labeling did not noticeably affect the cells’ ability to undergo osteogenic, chondrogenic, adipogenic, and neurogenic differentiation in vitro [ 33 , 34 ].…”

Section: Resultsmentioning

confidence: 99%

Methodological aspects of MRI of transplanted superparamagnetic iron oxide-labeled mesenchymal stem cells in live rat brain

et al. 2017

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In vivo tracking of transplanted mesenchymal stem cells (MSCs) migration and homing is vital for understanding the mechanisms of beneficial effects of MSCs transplantation in animal models of diseases and in clinical trials. Transplanted cells can be labeled with superparamagnetic iron oxide (SPIO) particles and visualized in vivo using a number of iron sensitive MRI techniques. However, the applicability of those techniques for SPIO-labeled MSCs tracking in live brain has not been sufficiently investigated. The goal of this study was to estimate the efficiency of various MRI techniques of SPIO-labeled cell tracing in the brain. To achieve that goal, the precision and specificity of T2WI, T2*WI and SWI (Susceptibility-Weighted Imaging) techniques of SPIO-labeled MSCs tracing in vitro and in live rat brain were for the first time compared in the same experiment. We have shown that SWI presents the most sensitive pulse sequence for SPIO-labeled MSCs MR visualization. After intracerebral administration due to limitations caused by local micro-hemorrhages the visualization threshold was 102 cells, while after intra-arterial transplantation SWI permitted detection of several cells or even single cells. There is just one publication claiming detection of individual SPIO-labeled MSCs in live brain, while the other state much lower sensitivity, describe detection of different cell types or high resolution tracing of MSCs in other tissues. This study confirms the possibility of single cell tracing in live brain and outlines the necessary conditions. SWI is a method convenient for the detection of single SPIO labeled MSCs and small groups of SPIO labeled MSCs in brain tissue and can be appropriate for monitoring migration and homing of transplanted cells in basic and translational neuroscience.

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Section: Resultsmentioning

confidence: 99%

Methodological aspects of MRI of transplanted superparamagnetic iron oxide-labeled mesenchymal stem cells in live rat brain

et al. 2017

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“…Since MSCs of different origin possess high migration ability and can undergo homing in various tissues and organs [47,48], expression of adhesion molecules on the cell surface represents an important phenotypic characteristic of these cells. To reach the parenchyma of the organ after systemic administration, MSCs should behave like leukocytes do during inflammation.…”

Section: Morphology and Phenotype Of Human Liver Mscsmentioning

confidence: 99%

Mesenchymal Stem Cells in the Adult Human Liver: Hype or Hope?

Холоденко

Курбатов

Холоденко

et al. 2019

Cells

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Chronic liver diseases constitute a significant economic, social, and biomedical burden. Among commonly adopted approaches, only organ transplantation can radically help patients with end-stage liver pathologies. Cell therapy with hepatocytes as a treatment for chronic liver disease has demonstrated promising results. However, quality human hepatocytes are in short supply. Stem/progenitor cells capable of differentiating into functionally active hepatocytes provide an attractive alternative approach to cell therapy for liver diseases, as well as to liver-tissue engineering, drug screening, and basic research. The application of methods generally used to isolate mesenchymal stem cells (MSCs) and maintain them in culture to human liver tissue provides cells, designated here as liver MSCs. They have much in common with MSCs from other tissues, but differ in two aspects—expression of a range of hepatocyte-specific genes and, possibly, inherent commitment to hepatogenic differentiation. The aim of this review is to analyze data regarding liver MSCs, probably another type of liver stem/progenitor cells different from hepatic stellate cells or so-called hepatic progenitor cells. The review presents an analysis of the phenotypic characteristics of liver MSCs, their differentiation and therapeutic potential, methods for isolating these cells from human liver, and discusses issues of their origin and heterogeneity. Human liver MSCs are a fascinating object of fundamental research with a potential for important practical applications.

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“…Cultured skin fibroblast-like cells contain subpopulations capable of differentiation within and out of the boundaries of the mesodermal lineage: into adipocytes, osteoblasts, chondrocytes, smooth muscle cells, neurons, astrocytes, and insulin-producing cells. 109 , 112 , 116 – 120 Cultures of skin plastic adherent cells contain cells originating from different structures forming this complex organ including dermis, adipose tissue, hair follicles, sweat and sebaceous glands, and others. It should be noted that fibroblast-like cells derived from skin specimens taken from the skull are partly of ectoderm origin because during embryogenesis in this area dermis is formed from the neural crest.…”

Section: Hepatogenic Differentiation Of Ipscmentioning

confidence: 99%

Cell-based therapies of liver diseases: age-related challenges

Yarygin¹,

Lupatov²,

Холоденко³

2015

CIA

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The scope of this review is to revise recent advances of the cell-based therapies of liver diseases with an emphasis on cell donor’s and patient’s age. Regenerative medicine with cell-based technologies as its integral part is focused on the structural and functional restoration of tissues impaired by sickness or aging. Unlike drug-based medicine directed primarily at alleviation of symptoms, regenerative medicine offers a more holistic approach to disease and senescence management aimed to achieve restoration of homeostasis. Hepatocyte transplantation and organ engineering are very probable forthcoming options of liver disease treatment in people of different ages and vigorous research and technological innovations in this area are in progress. Accordingly, availability of sufficient amounts of functional human hepatocytes is crucial. Direct isolation of autologous hepatocytes from liver biopsy is problematic due to related discomfort and difficulties with further expansion of cells, particularly those derived from aging people. Allogeneic primary human hepatocytes meeting quality standards are also in short supply. Alternatively, autologous hepatocytes can be produced by reprogramming of differentiated cells through the stage of induced pluripotent stem cells. In addition, fibroblasts and mesenchymal stromal cells can be directly induced to undergo advanced stage hepatogenic differentiation. Reprogramming of cells derived from elderly people is accompanied by the reversal of age-associated changes at the cellular level manifesting itself by telomere elongation and the U-turn of DNA methylation. Cell reprogramming can provide high quality rejuvenated hepatocytes for cell therapy and liver tissue engineering. Further technological advancements and establishment of national and global registries of induced pluripotent stem cell lines homozygous for HLA haplotypes can allow industry-style production of livers for immunosuppression-free transplantation.

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Functional Properties of Mesenchymal Stem Cells Labeled with Magnetic Microparticles in Vitro and Analysis of Their Distribution after Transplantation

Cited by 7 publications

References 9 publications

Methodological aspects of MRI of transplanted superparamagnetic iron oxide-labeled mesenchymal stem cells in live rat brain

Methodological aspects of MRI of transplanted superparamagnetic iron oxide-labeled mesenchymal stem cells in live rat brain

Mesenchymal Stem Cells in the Adult Human Liver: Hype or Hope?

Cell-based therapies of liver diseases: age-related challenges

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