Functional Proteomics Reveals the Biochemical Niche of C. elegans DCR-1 in Multiple Small-RNA-Mediated Pathways

Duchaîne, Thomas F.; Wohlschlegel, James A.; Kennedy, Scott; Bei, Yanxia; Conte, Darryl; Pang, Ka Ming; Brownell, Daniel R.; Harding, Sandra; Mitani, Shohei; Ruvkun, Gary; Yates, John R.; Mello, Craig C.

doi:10.1016/j.cell.2005.11.036

Cited by 356 publications

(551 citation statements)

References 41 publications

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“…There is an increasing number of reports that Argonautes associates with complexes that contain Dicer, the key enzyme in siRNA and miRNA maturation (Tabara et al, 2002;Sasaki et al, 2003;Okamura et al, 2004;Gregory et al, 2005;Maniataki and Mourelatos, 2005;Meister et al, 2005;Duchaine et al, 2006). The co-association of RISC and the miRNA processing is not surprising.…”

Section: Risc Formationmentioning

confidence: 99%

Principles and effects of microRNA-mediated post-transcriptional gene regulation

2006

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MicroRNAs (miRNAs) are abundant regulatory RNAs involved in the regulation of many key biological processes. Recent advances in understanding the mechanism of RNA interference and miRNA-mediated mechanisms shed light on major principals of the formation of the regulatory complex and provide models to explain how these small regulatory RNA species interfere with gene expression and how they influence the translational status of the transcriptome.

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Section: Risc Formationmentioning

confidence: 99%

Principles and effects of microRNA-mediated post-transcriptional gene regulation

2006

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“…The DexDh exonuclease eri-1 is an endogenous inhibitor of RNAi, whereas lin-15b is implicated in chromatin remodeling and also enhances the response to dsRNAs. eri-3 acts in a complex with eri-1 and like mutations in eri-1; eri-3 mutations enhance the response to dsRNAs that are not effective in wild type (Duchaine et al 2006).…”

Section: Identification Of Caenorhabditis Elegans Genes Regulatingmentioning

confidence: 99%

Identification ofCaenorhabditis elegansGenes Regulating Longevity Using Enhanced RNAi-sensitive Strains

Samuelson

Klimczak²,

Thompson³

et al. 2007

Cold Spring Harbor Symposia on Quantitative Biology

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A systematic genome-wide RNA interference screen was performed in the Caenorhabditis elegans lin-15b;eri-1 strain, which has an enhanced response to double-stranded RNA including the nervous system, to identify life-span regulatory factors. In total, 16,757 genes were examined, revealing 115 gene inactivations that extended life span. A more stringent longitudinal analysis revealed 18 gene inactivations that induced the greatest increase in life span (10-90%), all of which extended life span when inactivated either in eri-1 alone or in a second strain with an enhanced response to double-stranded RNA, eri-3. Most reduced the rate of aging, implying that animals aged more slowly. As was the case in previous studies, genes critical for metabolism caused the greatest extension of longevity. Extension of life span occurs through disparate mechanisms as increased resistance to thermal stress, oxidative damage, and decreased age pigment accumulation analysis of the 18 stronger positives failed to demonstrate a correlation between enhanced stress resistance and decreased lysosomal function. Consistently, aps-3 and lys-10, two genes annotated to have lysosomal functions, extended life span when inactivated without enhancing stress resistance. The results of this study reinforce the importance of metabolism, mitochondrial and lysosomal functions, genomic stability, and stress resistance on animal life-span determination.

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“…Because Eri1 encodes a DEDDh-type exonuclease with RNase activity toward the 3′ ends of RNA in vitro 1,[4][5][6] , it was proposed that Eri1 counteracts the RNAi pathway by degrading siRNAs 7 . Alternatively, Eri1 may inhibit RNAi indirectly by sequestering factors involved in siRNA processing into a different protein complex [8][9][10] .…”

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confidence: 99%

Mouse Eri1 interacts with the ribosome and catalyzes 5.8S rRNA processing

Ansel

Pastor

Rath

et al. 2008

Nat Struct Mol Biol

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Eri1 is a 3′-to-5′ exoribonuclease conserved from fission yeast to humans. Here we show that Eri1 associates with ribosomes and ribosomal RNA (rRNA). Ribosomes from Eri1-deficient mice contain 5.8S rRNA that is aberrantly extended at its 3′ end, and Eri1, but not a catalytically inactive mutant, converts this abnormal 5.8S rRNA to the wild-type form in vitro and in cells. In human and murine cells, Eri1 localizes to the cytoplasm and nucleus, with enrichment in the nucleolus, the site of preribosome biogenesis. RNA binding residues in the Eri1 SAP and linker domains promote stable association with rRNA and thereby facilitate 5.8S rRNA 3′ end processing. Taken together, our findings indicate that Eri1 catalyzes the final trimming step in 5.8S rRNA processing, functionally and spatially connecting this regulator of RNAi with the basal translation machinery.

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Functional Proteomics Reveals the Biochemical Niche of C. elegans DCR-1 in Multiple Small-RNA-Mediated Pathways

Cited by 356 publications

References 41 publications

Principles and effects of microRNA-mediated post-transcriptional gene regulation

Principles and effects of microRNA-mediated post-transcriptional gene regulation

Identification ofCaenorhabditis elegansGenes Regulating Longevity Using Enhanced RNAi-sensitive Strains

Mouse Eri1 interacts with the ribosome and catalyzes 5.8S rRNA processing

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