Functional Sperm Parameters and Fertility of Bull Semen Extended in Biociphos‐Plus® and Triladyl®

Abstract

ContentsTwenty ejaculates from _ve dairy AI!bulls were used to compare\ in a split!sample experiment\ the fertility ð45 day! non!return!rate "NRR# from more than 03999 AI# and sperm viability post!thaw of semen diluted with an egg yolk! "Tri! ladyl þ # or soybean!based "Biociphos!Plus þ # commercial extender[ The in vitro evaluations were divided in two experi! ments[ Experiment 0 "n 19# included post!thaw evaluations of motility "subjective and computerized#\ membrane integrity "CalceinAM:EthD!0\ SYBR!03:PI\ and osmotic resistance testÔ RT#\ and capacitation status "CTC:EthD!0#[ Experiment 1 "n 09# included evaluations of the capacitation!"CTC:EthD! 0# and acrosome status "FITC!PSA:EthD!0# during incubation with:without a challenge with solubilized zona pellucida pro! teins "SZP#[ No signi_cant di}erence in the fertility "58[0 2 9[7 versus 58[1 2 9[7# results was found between the two extenders[ In experiment 0\ the computerized motility evaluations post! thaw "CASA# showed higher values for Biociphos!Plus þ pro! cessed semen for the velocity patterns and lateral sperm head displacement[ After 5 h at room temperature "19Ð11>C# all the CASA motility patterns were signi_cantly higher for Biociphos! Plus þ [ The proportion of spermatozoa with intact membranes assessed by CalceinAM was signi_cantly higher in Biociphos! Plus þ "p ³ 9[990# compared to Triladyl þ \ but such di}erence was not seen when using SYBR!03 or the ORT!assay[ When using the CTC:EthD!0 assay\ a lower proportion of acrosome reacted "AR# spermatozoa post!thaw "p ³ 9[90# was found in Biociphos!Plus þ processed semen\ as well as a tendency "p ³ 9[96# for a higher number of uncapacitated spermatozoa[ In experiment 1\ the proportion of uncapacitated spermatozoa was signi_cantly higher for Biociphos!Plus þ when semen was incubated "27>C and 4) CO 1 # without SZP at both 9 "p ³ 9[990# and 29 min "p ³ 9[94#[ Concomitantly\ Triladyl þ showed a higher percentage of capacitated spermatozoa at 9 "p ³ 9[90#\ 29 "p ³ 9[94# and 019 min "p ³ 9[94#[ A higher "p ³ 9[94# incidence of AR!spermatozoa was seen in Triladyl þ at the beginning of the incubation with SZP[ No signi_cant di}erence between extenders was detected for the acrosome status by the FITC!PSA!assay[ Incubation with SZP induced acrosome reaction of capacitated spermatozoa in both extenders\ which was detected by CTC and FITC!PSA assays[ In conclusion\ fertility was not a}ected by Biociphos!Plus þ when 04 × 09 5 of spermatozoa per AI dose were inseminated[ The _nding that higher frequencies of spermatozoa seemed more membrane stable post!thaw\ when frozen in Biociphos! Plus þ \ might indicate that this extender better protects the sperm viability compared with Triladyl þ [

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

“…Freezing was performed using the controlled freezing methodology Direct Freezing in a freezer box Digitcool ® (IMV Cryo Bio System, L'Aigle, France). Muino et al 2007) and the 2-phase freezing curve described by Januskauskas et al (1999) and Gil et al (2000) were applied.…”

Efeito da utilização de diferentes diluidores para a produção in vitro de embriões bovinos

“…Em relação à motilidade espermática, os resultados encontrados neste experimento (não se observou diferença significativa entre os tratamentos) diferem dos achados de Janett et al (2005), que relatam o aumento da motilidade com a utilização de diluidor à base de lecitina de soja ao ser comparado com o composto à base de gema de ovo. Os valores médios apresentados para VAP, VSL, VCL e ALH no presente estudo corroboram os resultados obtidos por Gil et al (2000). Diversas pesquisas descrevem que a porcentagem elevada de VAP, VSL, VCL e ALH, encontrada na análise do sêmen pelo sistema CASA, condiz com a hiperativação espermática.…”

“…Quando se compararam os valores médios entre os diluidores, não se verificou diferença significativa (P>0,05), corroborando os resultados obtidos por Gil et al (2000).…”

Effect of low-density lipoprotein addition to soybean lecithin-based extenders on bull spermatozoa following freezing-thawing - preliminary results

“…However, studies evaluating the efficiency of bovine semen extenders based on soybean lecithin or egg yolk have shown varied results, in favour of the latter type of extender (e.g. Gil et al 2000;van Wagtendonk-de Leeuw et al 2000;Thun et al 2002;Aires et al 2003;Beran et al 2012;Crespilho et al 2012). Another alternative to egg yolk in extenders is its substitution only by the compound responsible for its cryoprotective properties.…”