Functional tooth restoration by next-generation bio-hybrid implant as a bio-hybrid artificial organ replacement therapy

Oshima, Masamitsu; Inoue, Katsuro; Nakajima, Kei; Tachikawa, Tetsuhiko; Yamazaki, H.; Isobe, Tomohide; Sugawara, Ayaka; Ogawa, Miho; Tanaka, Chie; Saito, Masahiro; Kasugai, Shohei; Takano‐Yamamoto, Teruko; Inoue, Takashi; Tezuka, Katsunari; Kuboki, Takuo; Yamaguchi, Akira; Tsuji, Takashi

doi:10.1038/srep06044

Cited by 69 publications

(63 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1, 2, 3, 4, 5 Because of their osteogenic capacity and tissue origin, hPDLSCs are considered to be excellent cellular source for alveolar bone regeneration and repair. 6, 7, 8, 9, 10, 11 However, changes in the microenvironment because of age, hypoxia and inflammation can impair the multiplex differentiation potential of hPDLSCs. 12, 13, 14, 15, 16 Periodontitis is a highly prevalent chronic inflammatory bone disease, and it is the main cause of tooth loss.…”

mentioning

confidence: 99%

Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Wang¹,

Song

et al. 2016

Cell Death Dis

146

View full text Add to dashboard Cite

Periodontitis impairs the osteogenic differentiation of human periodontal mesenchymal stem cells (hPDLSCs), but the underlying molecular mechanisms are still poorly understood. Long noncoding RNAs (lncRNAs) have been demonstrated to have significant roles under both physiologic and pathological conditions. In this study, we performed comprehensive lncRNA profiling by lncRNA microarray analysis and identified a novel lncRNA, osteogenesis impairment-related lncRNA of PDLSCs from periodontitis patients (lncRNA-POIR), the expression of which was significantly decreased in PDLSCs from periodontitis patients (pPDLSCs) and was upregulated by osteogenic induction. To study the functions of lncRNA-POIR, we prepared cells with overexpression and knockdown of lncRNA-POIR and found that lncRNA-POIR positively regulated osteogenic differentiation of hPDLSCs and pPDLSCs both in vitro and in vivo. Using quantitative real-time PCRs (qPCRs) and luciferase reporter assays, we demonstrated that lncRNA-POIR may act as a competing endogenous RNA (ceRNA) for miR-182, leading to derepression of its target gene, FoxO1. In this process, lncRNA-POIR and miR-182 suppress each other and form a network to regulate FoxO1. FoxO1 increased bone formation of pPDLSCs by competing with TCF-4 for β-catenin and inhibiting the canonical Wnt pathway. Finally, inflammation increases miR-182 expression through the nuclear factor-κB pathway, and the miR-182 overexpression in the inflammatory microenvironment resulted in an imbalance in the lncRNA-POIR-miR-182 regulatory network. In conclusion, our results provide novel evidence that this lncRNA-miRNA (microRNA) regulatory network has a significant role in osteogenic differentiation of pPDLSCs and that it has potential as a therapeutic target in mesenchymal stem cells during inflammation.

show abstract

mentioning

confidence: 99%

Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Wang¹,

Song

et al. 2016

Cell Death Dis

146

View full text Add to dashboard Cite

show abstract

“…Recently, novel tissue engineering methodologies have been developed termed bottom-up tissue engineering, which aims to address the challenges of recreating biomimetic structures by designing structural features on the microscale to build modular tissues that can in turn be used as building blocks to create larger tissues345. Understanding the proper structure of the target tissue and controlling the microenvironment may facilitate successful novel treatment strategies, as organ function involves biological cooperation with the surrounding tissues and other organs67.…”

mentioning

confidence: 99%

Three-dimensional ultrastructural analysis of cells in the periodontal ligament using focused ion beam/scanning electron microscope tomography

Hirashima

Ohta

Kanazawa

et al. 2016

Sci Rep

View full text Add to dashboard Cite

The accurate comprehension of normal tissue provides essential data to analyse abnormalities such as disease and regenerative processes. In addition, understanding the proper structure of the target tissue and its microenvironment may facilitate successful novel treatment strategies. Many studies have examined the nature and structure of periodontal ligaments (PDLs); however, the three-dimensional (3D) structure of cells in normal PDLs remains poorly understood. In this study, we used focused ion beam/scanning electron microscope tomography to investigate the whole 3D ultrastructure of PDL cells along with quantitatively analysing their structural properties and ascertaining their orientation to the direction of the collagen fibre. PDL cells were shown to be in contact with each other, forming a widespread mesh-like network between the cementum and the alveolar bone. The volume of the cells in the horizontal fibre area was significantly larger than in other areas, whereas the anisotropy of these cells was lower than in other areas. Furthermore, the orientation of cells to the PDL fibres was not parallel to the PDL fibres in each area. As similar evaluations are recognized as being challenging using conventional two-dimensional methods, these novel 3D findings may contribute necessary knowledge for the comprehensive understanding and analysis of PDLs.

show abstract

“…Additionally, bioengineered teeth must be sufficiently vascularized and integrated within the recipient anatomy. Overcoming these challenges may eventually contribute to emerging alternatives such as is bio-hybrid teeth, composed of both bioengineered living tissue and artificial materials [20, 82] • .…”

Section: Current Progress and Challenges In Whole Tooth Regenerationmentioning

confidence: 99%

Progress in Bioengineered Whole Tooth Research: from Bench to Dental Patient Chair

Smith

Yelick

2016

Curr Oral Health Rep

View full text Add to dashboard Cite

Tooth loss is a significant health issue that affects the physiological and social aspects of everyday life. Missing teeth impair simple tasks of chewing and speaking, and can also contribute to reduced self-confidence. An emerging and exciting area of regenerative medicine based dental research focuses on the formation of bioengineered whole tooth replacement therapies that can provide both the function and sensory responsiveness of natural teeth. This area of research aims to enhance the quality of dental and oral health for those suffering from tooth loss. Current approaches use a combination of dental progenitor cells, scaffolds and growth factors to create biologically based replacement teeth to serve as improved alternatives to currently used artificial dental prosthetics. This article is an overview of current progress, challenges, and future clinical applications of bioengineered whole teeth.

show abstract

Functional tooth restoration by next-generation bio-hybrid implant as a bio-hybrid artificial organ replacement therapy

Cited by 69 publications

References 48 publications

Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Three-dimensional ultrastructural analysis of cells in the periodontal ligament using focused ion beam/scanning electron microscope tomography

Progress in Bioengineered Whole Tooth Research: from Bench to Dental Patient Chair

Contact Info

Product

Resources

About