Functionalised alginate flow seeding microparticles for use in Particle Image Velocimetry (PIV)

Varela, Sylvana; Balagué, Isaac; Sancho, Irene; Ertürk, Nihal; Ferrando, Montserrat; Vernet, Antón

doi:10.3109/02652048.2016.1142016

Cited by 3 publications

(5 citation statements)

References 21 publications

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“…Within the 7% and 42% samples no individual RBC was visible, due to the opaqueness of blood. It has already been shown, that the alginate microspheres are well suited as tracer particles in PIV and PLIF at low particle fractions and even fluorescein or other dyes can be added to further improve the traceability (Kertzscher et al 2008;Ertürk et al 2013;Varela et al 2016). By using deformable microspheres, the results would be more comparable to the actual cell movement.…”

Section: Discussionmentioning

confidence: 99%

“…They have already been used with larger diameter as sufficient tracer particles with a small particle fraction to investigate near wall flows (wall Particle Image Velocimetry (PIV)) (Kertzscher et al 2008). Ertürk et al (2013) used approximately 20 μm alginate microspheres with fluorescein as tracer particles in oil and air flow and Varela et al (2016) functionalized them with Rhodamin B and showed the applicability in PIV and Planar Laser-Induced Fluorescence (PLIF). The microspheres are suitable to follow the flow in a liquid as their porous structure allows them to take on the density of the surrounding fluid (Ertürk et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Flow study on a transparent two-phase blood model fluid based on alginate microspheres

et al. 2022

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The reduction of blood damage is still a big challenge in blood-carrying medical devices. In vitro experiments are performed to investigate the damage-causing effects, but due to the opaqueness of blood cells, only near-wall flows can be observed. Thus, several transparent blood models to visualize the rheologic behavior of blood have been proposed and examined. Nevertheless, two-phase blood models with added particles still represent the properties of blood inadequately or are very expensive and complex to produce. In this in vitro study, the viscosity, the flow behavior and the cell deformation of human red blood cells have been compared to a novel, easy-to-produce, two-phase blood model fluid with deformable alginate microspheres. The comparison has been performed in a cone-plate rheometer, a straight and a hyperbolic converging microchannel. The viscosity of the blood model fluid with a particle fraction of 30% showed a shear-thinning behavior, comparable to that of blood at room and human body temperature within shear rates from 7 to $$2000\,\hbox {s}^{-1}$$ 2000 s - 1 . The alginate microspheres were deformable in an extensional flow and formed a cell free layer comparable to that of blood in a straight microchannel. The experiments showed a good optical accessibility of the two-phase flow with traceable movements of individual microspheres in the center of the microchannel. It could be shown that our proposed blood model fluid is a promising tool for the analysis of two-phase flows in complex flow geometries. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Flow study on a transparent two-phase blood model fluid based on alginate microspheres

et al. 2022

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“…It was possible to detect the movements of individual microspheres at a particle fraction of 5 % in the center of the microchannel. It has already been shown, that the alginate microspheres are well suited as tracer particles in PIV and PLIF at low particle fractions and even fluorescein or other dyes can be added to further improve the traceability (Kertzscher et al, 2008;Ertürk et al, 2013;Varela et al, 2016). By using deformable microspheres, the results would be more comparable to the actual cell movement.…”

Section: Discussionmentioning

confidence: 99%

“…They have already been used with larger diameter as sufficient tracer particles with a small particle fraction to investigate near wall flows (wall Particle Image Velocimetry (PIV)) (Kertzscher et al, 2008). Ertürk et al (2013) used approximately 20 µm alginate microspheres with fluorescein as tracer particles in oil and air flow and Varela et al (2016) functionalized them with Rhodamin B and showed the applicability in PIV and Planar Laser-Induced Fluorescence (PLIF). The microspheres are suitable to follow the flow in a liquid as their porous structure allows them to take on the density of the surrounding fluid (Ertürk et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Flow Study on a Transparent Two-Phase Blood Model Fluid Based on Alginate Microspheres

Froese

Gabel

Parnell

et al. 2022

Preprint

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The reduction of blood damage is still a big challenge in blood-carrying medical devices. In-vitro experiments are performed to investigate the damage-causing effects, but due to the opaqueness of blood cells, only near-wall flows can be observed. Thus, several transparent blood models to visualize the rheologic behavior of blood have been proposed and examined. Nevertheless, two-phase blood models with added particles still represent the properties of blood inadequately or are very expensive and complex to produce. In this in-vitro study, the viscosity, the flow behavior and the cell deformation of human red blood cells (RBCs) have been compared to a novel, easy-to-produce, two-phase blood model fluid with deformable alginate microspheres. The comparison has been performed in a cone-plate rheometer, a straight and a hyperbolic converging microchannel. The viscosity of the blood model fluid with a particle fraction of 30 % showed a shear-thinning behavior, comparable to that of blood at room and human body temperature within shear rates from 7 s-1 to 2000 s-1. The alginate microspheres were deformable in an extensional flow and formed a cell free layer (CFL) comparable to that of blood in a straight microchannel. The experiments showed a good optical accessibility of the two-phase flow with traceable movements of individual microspheres in the center of the microchannel. It could be shown that our proposed blood model fluid is a promising tool for the analysis of two-phase flows in complex flow geometries.

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“…Further details are provided by Ertürk et al [16], where it is demonstrated that alginate particles are very suitable for PIV application in turbo-machinery, on the mineral oil is the principal working fluid. More recently, Varela et al [25] explained in more details other PIV applications where the capability of controlling the diameter and their porosity provide great advantages in front of other seeders like air-bubbles [15]. Gaussian geometric optics limit the smallest size of the distinguishable particle.…”

Section: Particle Piv and Working Fluid Requirementsmentioning

confidence: 99%

Experimental study on the impulsion port of a trochoidal wheeled pump

Raush

Gamez-Montero

Castilla

et al. 2017

Flow Measurement and Instrumentation

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© 2017. This version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/All positive displacement pumps produce a pulsating flow. The present paper reports the experimental measurement of steady flow pulsations in the outlet of the internal wheeled pump. In the measured flow, the manufacturing tolerance are responsible of part of the spectra of the whole pulsation. Time-Resolved Particle Image Velocimetry technique has been used for this purpose. The flow pulsation measurement from a direct visualization of the velocity profile was carried out. The flow rate signal is derived from ad-hoc integration algorithm of the radial velocity profile, where the area discretization is a constant parameter that is relevant to minimize PIV errors by velocity gradients regions near the wall. Spectrographic analysis on the experimental data reveled low frequency components related with manufacturing tolerances. Measurements of this non-invasive procedure are compared with detailed CFD numerical results obtained from an improved gerotor model where manufacturing tolerances have been included. To be compared, cross-power spectral density analysis has been applied. The results reported in the paper show a method to provide a fast non-invasive flow pulsation measurement not only for pumps but also could be extended to compare aging effects of other kind of fluid power devices.Peer ReviewedPostprint (author's final draft

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Functionalised alginate flow seeding microparticles for use in Particle Image Velocimetry (PIV)

Cited by 3 publications

References 21 publications

Flow study on a transparent two-phase blood model fluid based on alginate microspheres

Flow study on a transparent two-phase blood model fluid based on alginate microspheres

Flow Study on a Transparent Two-Phase Blood Model Fluid Based on Alginate Microspheres

Experimental study on the impulsion port of a trochoidal wheeled pump

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