Functionalised gold nanoparticles for selective induction of<i>in vitro</i>apoptosis among human cancer cell lines

Mohan, Jithin C.; Praveen, G; Chennazhi, K.P.; Jayakumar, R.; Nair, Shantikumar V.

doi:10.1080/17458080.2011.557841

Cited by 60 publications

(38 citation statements)

References 23 publications

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“…The peaks at 1636 cm −1 and 3254 cm −1 can be assigned to the O-H scissor bonds and O-H stretching bonds, respectively. These data confirm that citrate served as a capping agent in Au-NPs [41].…”

Section: Physical Properties Of Au-npssupporting

confidence: 77%

Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening

et al. 2020

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Biocompatibility is very important for cell growth using 3D printers, but biocompatibility materials are very expensive. In this study, we investigated the possibility of cell culture by the surface modification of relatively low-cost industrial materials and an efficient three-dimensional (3D) scaffold made with an industrial ABS filament for cell proliferation, spheroid formation, and drug screening applications. We evaluated the adequate structure among two-layer square shape 3D scaffolds printed by fused deposition modeling with variable infill densities (10–50%). Based on the effects of these scaffolds on cell proliferation and spheroid formation, we conducted experiments using the industrial ABS 3D scaffold (IA3D) with 40% of infill density, which presented an external dimension of (XYZ) 7650 µm × 7647 µm × 210 µm, 29.8% porosity, and 225 homogenous micropores (251.6 µm × 245.9 µm × 210 µm). In the IA3D, spheroids of cancer HepG2 cells and keratinocytes HaCaT cells appeared after 2 and 3 days of culture, respectively, whereas no spheroids were formed in 2D culture. A gold nanoparticle-coated industrial ABS 3D scaffold (GIA3D) exhibited enhanced biocompatible properties including increased spheroid formation by HepG2 cells compared to IA3D (1.3-fold) and 2D (38-fold) cultures. Furthermore, the cancer cells exhibited increased resistance to drug treatments in GIA3D, with cell viabilities of 122.9% in industrial GIA3D, 40.2% in IA3D, and 55.2% in 2D cultures when treated with 100 µM of mitoxantrone. Our results show that the newly engineered IA3D is an innovative 3D scaffold with upgraded properties for cell proliferation, spheroid formation, and drug-screening applications.

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Section: Physical Properties Of Au-npssupporting

confidence: 77%

Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening

et al. 2020

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“…Once further modified with PSS and PDDAC, the cytotoxity of Au NPs was significantly reduced [79]. Another study also showed that citrate-coated Au NPs induce apoptosis in human lung cancer A549 cells, but Au NPs coated with polyethylenimine (PEI) showed negligible toxicity to both A549 cells and MCF-7 cells [107]. Another study showed that the cytotoxicity of triphenylphosphine monosulfonate-modified Au NPs of 1.4 nm (Au1.4MS) and glutathione-modified NPs of 1.1 nm (Au1.1GSH) was different.…”

Section: Interaction Of Au Nps With Cellsmentioning

confidence: 99%

Interaction of gold nanoparticles with proteins and cells

Wang

et al. 2015

Science and Technology of Advanced Materials

168

117

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Gold nanoparticles (Au NPs) possess many advantages such as facile synthesis, controllable size and shape, good biocompatibility, and unique optical properties. Au NPs have been widely used in biomedical fields, such as hyperthermia, biocatalysis, imaging, and drug delivery. The broad application range may result in hazards to the environment and human health. Therefore, it is important to predict safety and evaluate therapeutic efficiency of Au NPs. It is necessary to establish proper approaches for the study of toxicity and biomedical effects. In this review, we first focus on the recent progress in biological effects of Au NPs at the molecular and cellular levels, and then introduce key techniques to study the interaction between Au NPs and proteins. Knowledge of the biomedical effects of Au NPs is significant for the rational design of functional nanomaterials and will help predict their safety and potential applications.

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“…The peak values corresponding to the C-O stretching shifted from 1055 to 1061 cm -1 due to presented citrate salts. The symmetric and anti-symmetric stretching of COOat 1395 and 1576 cm -1 at FTIR spectra of MC8SC3 hydrogel sample was also support the interaction of citrate ions and MC (9). SEM micrographs reveal the interconnected porous microstructure.…”

Section: Discussionmentioning

confidence: 52%

“…Functional groups of the synthesized hydrogels were detected by using FTIR spectrometer from 4000 to 400 cm-1 with Thermo Scientific Smart Orbit model (9). The morphology and microstructure of the lyophilized hydrogels were examined by using scanning electron microscopy (SEM) (Zeiss, Evo LS10) with 10kV accelerating voltage at Yıldız Technical University, Istanbul, Turkey (30).…”

Section: Methodsmentioning

confidence: 99%

Effect of Concentration of Citric Acid on The Rheological Properties of Methylcellulose Hydrogels

Oğuz¹,

Ege²

2018

atfm

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Functionalised gold nanoparticles for selective induction ofin vitroapoptosis among human cancer cell lines

Cited by 60 publications

References 23 publications

Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening

Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening

Interaction of gold nanoparticles with proteins and cells

Effect of Concentration of Citric Acid on The Rheological Properties of Methylcellulose Hydrogels

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