Functionality integration in stereolithography 3D printed microfluidics using a “print-pause-print” strategy

Sagot, Matthieu; Derkenne, Timothée; Giunchi, Perrine; Davit, Yohan; Nougayrède, Jean-Philippe; Tregouet, Corentin; Raimbault, Vincent; Malaquin, Laurent; Venzac, Bastien

doi:10.1039/d4lc00147h

Lab Chip

2024

DOI: 10.1039/d4lc00147h

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Functionality integration in stereolithography 3D printed microfluidics using a “print-pause-print” strategy

Matthieu Sagot,

Timothée Derkenne,

Perrine Giunchi

et al.

Abstract: Novel protocol for integration of object inside stereolithography-based chips and proof-of-concepts: bacteria imaging, blue energy and in-flow cell capture.

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Stacked hydrogel-based brain-on-chips utilizing capillary force flow pinning

Akcay,

van Venrooij,

Luttge

2024

Journal of Vacuum Science &Amp; Technology B

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Cellular processes in brain tissue such as migration, proliferation, morphology, and differentiation are influenced by mechanical cues, demonstrating the interplay between the structure and function. Given the complexity, it remains a substantial challenge to establish a reliable in vitro model mimicking structural properties as brain tissue. To address this challenge, we propose an innovative approach to create vertical hydrogel stacks based on microfluidic technology. 3D-printed microfluidic features in the sidewall profile of our chip designs allowed us to faithfully replicate these capillary force flow pinning structures in polydimethylsiloxane. After the successful application of hydrogel of a defined height, thanks to the pinning process and plating of stem-cell-derived neurons, the results demonstrated the potential of our BoC platform, providing a valuable tool for neuroscience research.

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Stacked hydrogel-based brain-on-chips utilizing capillary force flow pinning

Akcay,

van Venrooij,

Luttge

2024

Journal of Vacuum Science &Amp; Technology B

View full text Add to dashboard Cite

show abstract

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Functionality integration in stereolithography 3D printed microfluidics using a “print-pause-print” strategy

Abstract: Novel protocol for integration of object inside stereolithography-based chips and proof-of-concepts: bacteria imaging, blue energy and in-flow cell capture.

Cited by 1 publication

References 44 publications

Stacked hydrogel-based brain-on-chips utilizing capillary force flow pinning

Stacked hydrogel-based brain-on-chips utilizing capillary force flow pinning

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