Functionalization of gold screen printed electrodes with bacterial photosynthetic reaction centers by laser printing technology for mediatorless herbicide biosensing

Chatzipetrou, Marianneza; Milano, Francesco; Giotta, Livia; Chirizzi, Daniela; Trotta, Massimo; Massaouti, Maria; Guascito, Maria Rachele; Zergioti, I.

doi:10.1016/j.elecom.2016.01.008

Cited by 39 publications

(35 citation statements)

References 23 publications

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“…SPEs are highly-versatile, easy to use, cost-effective analytical tools, also suitable to miniaturization [23,24]. Furthermore, a screen printed electrode avoids the cleaning process, unlike conventional electrodes such as a glassy carbon electrode (GCE) [25].…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide/ZnO Nano Composite for Sensitive and Selective Electrochemical Sensing of Levodopa and Tyrosine Using Modified Graphite Screen Printed Electrode

2016

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Determination of levodopa and tyrosine as two important species for treatment of Parkinson's disease is described. A novel electrochemical sensor involving graphene oxide/ZnO nanorods (GR/ZnO) nano composite and the graphite screen‐printed electrodes (GSPE) was developed for the simultaneous detection of levodopa and tyrosine. The screen‐printed electrodes with several advantages, including low cost, versatility and miniaturization were employed. On the other hand, the graphene oxide/ZnO nanorods nano composite was casted on the surface of GSPE to obtain GR/ZnO/SPE. The proposed nano sensor has excellent performance such as high sensitivity, good selectivity and analytical application in real samples. The combination of graphene oxide/ZnO nanorods nano composite with the screen‐printed electrode is favorable for amplifying electrochemical signals. Under optimized conditions square wave voltammetry (SWV) exhibited linear dynamic ranges from 1.0×10−6 to 1.0×10−3 M and 1.0×10−6 to 8.0×10−4 M with detection limits of 4.5×10−7 M and 3.4×10−7 M for levodopa and tyrosine respectively.

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Section: Introductionmentioning

confidence: 99%

Graphene Oxide/ZnO Nano Composite for Sensitive and Selective Electrochemical Sensing of Levodopa and Tyrosine Using Modified Graphite Screen Printed Electrode

2016

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“…The SPEs have been designed especially for miniaturization of electrochemical analytical systems [33]. SPEs are highlyversatile, easy to use, cost-effective analytical tools, also suitable to miniaturization [34]. Furthermore, a SPE avoids the cleaning process, unlike conventional electrodes such as a GCE [35].…”

Section: Introductionmentioning

confidence: 99%

Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine

Mohammadi

Beitollahi

Tajik

2018

Micro and Nano Syst Lett

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In the present study, a screen-printed electrode based-sensor with electrochemical detection was developed for rapid and sensitive determination of acetylcholine. At first, the screen-printed carbon electrode was modified by using a magnetic core shell and then was used for voltammetric determination of acetylcholine in ampoule, serum and urine samples. The electrochemical behaviour of acetylcholine at the modified electrode was investigated by cyclic voltammetry. The modified electrode displayed a decrease in the overpotential (ca. 130 mV) and an obvious increase in the peak current compared to the non-modified screen-printed electrode. The results indicated that modified screenprinted electrode enhanced electrocatalytic activity towards the oxidation of acetylcholine. Under optimized conditions, the limit of detection from the experiment of acetylcholine determination was 0.02 µM with acetylcholine concentration in range of 0.1-500.0 µM. The reproducibility of the measurements was tested by recording the responses for 50.0 µM acetylcholine with four different developed sensors prepared on the same manner and the same day. A relative standard deviation value of 4.3% was obtained. Finally, a recovery test is done as a part of accuracy evaluation of the system. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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“…1a, and see Fig. S1 in Supporting information) facilitates a highly-efficient photochemical charge separation that has been exploited for the construction of prototype solar cells [5], [6], [7], photoelectrochemical cells [8], [9], [10], [11], [12], [13], [14], [15], [16], [17], [18] and biosensors [19], [20]. Reaction centres from oxygenic phototrophs are of particular interest with regard to solar fuel synthesis through water splitting [21], [22] and the powering of catalysis by other redox proteins in non-native, hybrid systems [23], [24].…”

Section: Introductionmentioning

confidence: 99%

Directed assembly of defined oligomeric photosynthetic reaction centres through adaptation with programmable extra-membrane coiled-coil interfaces

Swainsbury

Harniman

Bartolo

et al. 2016

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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A challenge associated with the utilisation of bioenergetic proteins in new, synthetic energy transducing systems is achieving efficient and predictable self-assembly of individual components, both natural and man-made, into a functioning macromolecular system. Despite progress with water-soluble proteins, the challenge of programming self-assembly of integral membrane proteins into non-native macromolecular architectures remains largely unexplored. In this work it is shown that the assembly of dimers, trimers or tetramers of the naturally monomeric purple bacterial reaction centre can be directed by augmentation with an α-helical peptide that self-associates into extra-membrane coiled-coil bundle. Despite this induced oligomerisation the assembled reaction centres displayed normal spectroscopic properties, implying preserved structural and functional integrity. Mixing of two reaction centres modified with mutually complementary α-helical peptides enabled the assembly of heterodimers in vitro, pointing to a generic strategy for assembling hetero-oligomeric complexes from diverse modified or synthetic components. Addition of two coiled-coil peptides per reaction centre monomer was also tolerated despite the challenge presented to the pigment-protein assembly machinery of introducing multiple self-associating sequences. These findings point to a generalised approach where oligomers or longer range assemblies of multiple light harvesting and/or redox proteins can be constructed in a manner that can be genetically-encoded, enabling the construction of new, designed bioenergetic systems in vivo or in vitro.

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Functionalization of gold screen printed electrodes with bacterial photosynthetic reaction centers by laser printing technology for mediatorless herbicide biosensing

Cited by 39 publications

References 23 publications

Graphene Oxide/ZnO Nano Composite for Sensitive and Selective Electrochemical Sensing of Levodopa and Tyrosine Using Modified Graphite Screen Printed Electrode

Graphene Oxide/ZnO Nano Composite for Sensitive and Selective Electrochemical Sensing of Levodopa and Tyrosine Using Modified Graphite Screen Printed Electrode

Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine

Directed assembly of defined oligomeric photosynthetic reaction centres through adaptation with programmable extra-membrane coiled-coil interfaces

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