Functionalized carbon microarrays platform for high sensitive detection of HIV-Tat peptide

Abstract

Detection of HIV-TAT peptide on functionalized 3-dimensional carbon micropillar array platform with detection limit of 50 pmol.

Analytical methodologies using carbon substrates developed by pyrolysis

“…5, similar structures were later modied via vacuum and UV light to introduce -COOH groups and applied for the detection of a HIV peptide down to 50 pM levels, which is well-within the useful clinical range. 149 Taking advantage of their high affinity for carbon surfaces, 96 a number of biomolecules have been immobilized on the surface of various carbon substrates via adsorption. [150][151][152] Additionally, and because adsorption is a step required for most immobilization processes, much attention has been given to this process to maximize the overall activity of the biosensor.…”

Aptamer-Based Carboxyl-Terminated Nanocrystalline Diamond Sensing Arrays for Adenosine Triphosphate Detection

“…With strong oxidation by the VUV excimer laser, CH 3 becomes COOH. A high density of ozone and excited oxygen are required in the irradiation process to change CH 3 to COOH [ 34 , 36 ]. By VUV treatment, the average thickness of O is 0.6 monolayers.…”

“…For biomolecule activation, the carboxyl- and fluorine-terminated micropatterned NCD surface was activated in 0.1 M N -HydroxySuccinimide/0.4 M 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (1:1 volume ratio) for 1 h. The NCD surface was washed three times with deionized (DI) water and then dried before supporting-DNA immobilization. Washing was performed because it improves the immobilization capability [ 34 ]. The sequence of the supporting DNA complementary to the DNA aptamer is the 21-mer NH 2 -5′-CCACGGAC-TACTTCAAAACTA-3′ and the fluorescently labeled DNA aptamer has the sequence 3′-GGTGCCTG-ATGAAGTTTTGAT-5′-Cy5.…”