Functionalized magnetic nanobeads for SERS-based detection of <i>Staphylococcus aureus</i>

Ji, Shuaifeng; Xiang, Yunfeng; Han, Dianpeng; Liu, Chenghua; Du, Yuwan; Peng, Yuan; Li, Shuang; Ren, Shuyue; Kang, Qin; Wang, Yu; Zhou, Huanying; Jia, Zhenghong; Gao, Zhixian

doi:10.1039/d3ay00653k

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…Polyethylene glycol (PEG) and bovine serum albumin (BSA) dual-mediated teicoplanin-functionalized magnetic beads (TEI-BPBs) were used for the isolation of the target bacteria. TEI can recognize and capture Gram-positive bacteria, such as S. aureus , by binding to the D -Ala– D -Ala peptide fraction; however, it lacks some specificity in recognizing the bacterial cells [ 234 ]. Therefore, SERS tags were used to immobilize antibodies on gold surfaces via bifunctional linker proteins to ensure specific recognition of S. aureus .…”

Section: Isolation Of Bacteriamentioning

confidence: 99%

“…Following separation, the bacteria undergo Raman spectroscopy to acquire their unique spectral signatures. A novel method for detecting S. aureus, a common foodborne pathogen, was developed by Ji et al [234], using functionalized magnetic beads and surface-enhanced Raman scattering (SERS) tags. Polyethylene glycol (PEG) and bovine serum albumin (BSA) dualmediated teicoplanin-functionalized magnetic beads (TEI-BPBs) were used for the isolation of the target bacteria.…”

Section: Sample Processing: Isolation From Matrix and Isolation Strat...mentioning

confidence: 99%

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Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms

Frempong,

Salbreiter,

Mostafapour

et al. 2024

Molecules

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Raman spectroscopy is an emerging method for the identification of bacteria. Nevertheless, a lot of different parameters need to be considered to establish a reliable database capable of identifying real-world samples such as medical or environmental probes. In this review, the establishment of such reliable databases with the proper design in microbiological Raman studies is demonstrated, shining a light into all the parts that require attention. Aspects such as the strain selection, sample preparation and isolation requirements, the phenotypic influence, measurement strategies, as well as the statistical approaches for discrimination of bacteria, are presented. Furthermore, the influence of these aspects on spectra quality, result accuracy, and read-out are discussed. The aim of this review is to serve as a guide for the design of microbiological Raman studies that can support the establishment of this method in different fields.

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Section: Isolation Of Bacteriamentioning

confidence: 99%

Section: Sample Processing: Isolation From Matrix and Isolation Strat...mentioning

confidence: 99%

Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms

Frempong,

Salbreiter,

Mostafapour

et al. 2024

Molecules

View full text Add to dashboard Cite

show abstract

“…The LOD was as low as 1.09 CFU/mL and the detection time is as short as 50 min. The sandwich detection strategy based on two nanocomposites have also received attention from other researchers. , Researchers are more focused on the development of nanomaterials that enhance SERS performance.…”

Section: Optical Biosensorsmentioning

confidence: 99%

Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus

Wang,

Yang

2024

J. Agric. Food Chem.

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Staphylococcus aureus (S. aureus) is a kind of pathogenic bacteria which can lead to food poisoning, hospital, and community infections. S. aureus and methicillin-resistant S. aureus (MRSA) have become headaches for public health worldwide. Therefore, strengthening the detection of S. aureus and MRSA is a critical step to prevent and control its spread and infection. This review summarized multiple detection methods (electrochemical, optical, and other biosensors) for sensitive and efficient detection of nonresistant and resistant S. aureus. First, we have introduced the principle and methods of detection platform for S. aureus and MRSA. We also contrasted various detection strategies. Finally, the current situation and prospect of S. aureus and MRSA detection in the future are explored in depth, and its development direction of detection methods is also predicted. In this review, we found that although biosensors have shown tremendous brilliance in the field of monitoring, they are currently in the experimental stage. It can be certain that we are very close to entering the commercialization stage. The point-of care testing available to nonprofessionals will become a new direction. We firmly believe that the monitoring system will be more perfect and stable and public life will be healthier and safer.

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SERS-based Ag NCs@PDMS flexible substrate combined with chemometrics for rapid detection of foodborne pathogens on egg surface

Peng,

Zhang,

Xin

et al. 2024

Microchim Acta

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Functionalized magnetic nanobeads for SERS-based detection of Staphylococcus aureus

Abstract: We introduced a Raman detection technique based on a combination of functionalised magnetic beads and surface-enhanced Raman scattering (SERS) tags to develop a rapid and sensitive strategy for the detection of Staphylococcus aureus.

Cited by 5 publications

References 39 publications

Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms

Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms

Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus

SERS-based Ag NCs@PDMS flexible substrate combined with chemometrics for rapid detection of foodborne pathogens on egg surface

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