Functionalized magnetite nanoparticles with <i>Moringa oleifera</i> with potent antibacterial action in wastewater

Oliveira, Alessandra Marjorie de; Mateus, Gustavo Affonso Pisano; Santos, Tássia Rhuna Tonial dos; Filho, Benício Alves de Abreu; Gomes, Raquel Guttierres; Bergamasco, Rosângela

doi:10.1080/09593330.2020.1754923

Cited by 16 publications

(6 citation statements)

References 46 publications

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“…However, dissolution of the physically adsorbed CS/AgNPs from the cork surface could have affected the antibacterial performance of the material. In contrast, the work by De Oliveira et al [46] evaluated the functionalization with M. oleifera of magnetic nanoparticles (Fe 3 O 4 ) for S. aureus removal and showed that the antibacterial effect was more efficient when using a combination of M. oleifera and magnetite (Fe-Fe 2 O 4 ) with constant removal rates compared to Fe-Fe 2 O 4 or M. oleifera acting alone, since the functionalization of Fe-Fe 2 O 4 with M. oleifera produced a coagulant within the surface area containing functional groups and minerals. That antibacterial effect was consistent with our study, where the combination of MoSe and cork improved the adsorption and InhEc efficiency.…”

Section: Functionalization Of Cork With Mose and Inhecmentioning

confidence: 83%

Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli

González

Rodríguez

Bartolo

et al. 2021

Water

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Cork matrices biofunctionalized with Moringa oleifera seed extracts (MoSe) have potential for use as a biofilter with antibacterial properties to reduce waterborne pathogens. The aim of this study was to evaluate the effect of cork biofunctionalized with active antimicrobial compounds of MoSe (f-cork) on the inhibition of Escherichia coli (InhEc). The LacZ gene from a strain of E. coli was used as the target sequence using viability quantification Polymerase Chain Reaction (qPCR) and differentiation of viable and dead bacteria through selective cell viability PMA staining. To perform this, a 27−4 fractional factorial design and a biofiltration system were used to evaluate the effect of the active protein in MoSe immobilized in granulated cork on InhEc. We found that the potential for antimicrobial activity increased with f-cork for an effective maximal bacterial reduction (99.99%; p < 0.05). The effect of f-cork functionalized with MoSe on E. coli viability was of 0.024% and 0.005% for the cells exposed to PMA, respectively, being the relevant conditions in treatment 2: (0 L/min) without aeration, (5%) MoSe and (5 mm) cork particle. In conclusion, the f-cork functionalized with MoSe presented biosorbent and antibacterial properties that effectively reduced the E. coli growth.

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Section: Functionalization Of Cork With Mose and Inhecmentioning

confidence: 83%

Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli

González

Rodríguez

Bartolo

et al. 2021

Water

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“…FeNPs are extensively used for applications in drug delivery [ 26 ], treatment of cancer cells [ 27 ], antimicrobials [ 28 , 29 , 30 ], and in general biomedicine and bioanalytics [ 31 ]. FeNPs are also now use for antimicrobial applications due to their very small in size and high surface area to volume [ 32 , 33 ]. FeNPs disseminate ionic iron II and iron III species with low toxicity to eukaryotic host cells minimizing undesirable side effects [ 29 , 34 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Experimental Evolution of Magnetite Nanoparticle Resistance in Escherichia coli

et al. 2021

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Both ionic and nanoparticle iron have been proposed as materials to control multidrug-resistant (MDR) bacteria. However, the potential bacteria to evolve resistance to nanoparticle bacteria remains unexplored. To this end, experimental evolution was utilized to produce five magnetite nanoparticle-resistant (FeNP1–5) populations of Escherichia coli. The control populations were not exposed to magnetite nanoparticles. The 24-h growth of these replicates was evaluated in the presence of increasing concentrations magnetite NPs as well as other ionic metals (gallium III, iron II, iron III, and silver I) and antibiotics (ampicillin, chloramphenicol, rifampicin, sulfanilamide, and tetracycline). Scanning electron microscopy was utilized to determine cell size and shape in response to magnetite nanoparticle selection. Whole genome sequencing was carried out to determine if any genomic changes resulted from magnetite nanoparticle resistance. After 25 days of selection, magnetite resistance was evident in the FeNP treatment. The FeNP populations also showed a highly significantly (p < 0.0001) greater 24-h growth as measured by optical density in metals (Fe (II), Fe (III), Ga (III), Ag, and Cu II) as well as antibiotics (ampicillin, chloramphenicol, rifampicin, sulfanilamide, and tetracycline). The FeNP-resistant populations also showed a significantly greater cell length compared to controls (p < 0.001). Genomic analysis of FeNP identified both polymorphisms and hard selective sweeps in the RNA polymerase genes rpoA, rpoB, and rpoC. Collectively, our results show that E. coli can rapidly evolve resistance to magnetite nanoparticles and that this result is correlated resistances to other metals and antibiotics. There were also changes in cell morphology resulting from adaptation to magnetite NPs. Thus, the various applications of magnetite nanoparticles could result in unanticipated changes in resistance to both metal and antibiotics.

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“…Moreover, magnetite has a high surface area and can act as an adsorbent, further enhancing its ability to remove contaminants from wastewater. 91,92 Chitosan has been shown to have excellent occulation properties and is effective in removing suspended solids and other pollutants from wastewater. When modied with magnetic particles, chitosan can be easily separated from the treated wastewater using magnetic elds.…”

Section: Magnetic Natural Coagulants/ Flocculants (Mn-c/fs)mentioning

confidence: 99%

Natural-based coagulants/flocculants as sustainable market-valued products for industrial wastewater treatment: a review of recent developments

2023

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Functionalized magnetite nanoparticles with Moringa oleifera with potent antibacterial action in wastewater

Cited by 16 publications

References 46 publications

Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli

Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli

Experimental Evolution of Magnetite Nanoparticle Resistance in Escherichia coli

Natural-based coagulants/flocculants as sustainable market-valued products for industrial wastewater treatment: a review of recent developments

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