Functionalized monolithic columns: Recent advancements and their applications for high-efficiency separation and enrichment in food and medicine

Abstract

The chromatographic column is the core of a high-performance liquid chromatography (HPLC) system, and must have excellent separation efficiency and selectivity. Therefore, functional modification materials for monolithic columns have been rapidly developed. This study is a systematic review of the recently reported functionalized monolithic columns. In particular, the study reviews the types of functional monomers under different modification conditions, as well as the separation and detection techniques combined with chromatography, and their development prospects. In addition, the applications of functionalized monolithic columns in food analysis, biomedicine, and the analysis of active ingredient of Chinese herbal medicines in recent years are also discussed. Also reviewed are the functionalized monolithic columns for qualitative and quantitative analysis. It provided a reference for further development and application of organic polymer monolithic columns.

Research of saccharides and related biocomplexes: A review with recent techniques and applications

“…In general, silica-based monoliths with Si-OR functionalities have stability at extremely high temperatures. They suited well for monosaccharides and showed high efficiency, although principally aminobonded materials are used for their studies [148,149]. In LC-MS/MS monolithic sorbents allowed the identification and quantification of monoclonal antibodies (mAbs), glycopeptides [15,80], and glycans [62,80].…”

Functionalized cellulose monolith based affinity chromatography columns for efficient separation of protein molecules

“…18 Most of the desirable requirements of a carrier such as homogeneity, macroporosity, hydrophilicity, chemical/physical stability, suppression of non-specific adsorption, good liquid permeability, high surface area for ligand immobilization and modification and ease of handling 5 can be achieved by using a monolith. Different types of functionalized monolithic columns with high separation efficiency have been reported 19 of which some typical carriers include silica, 20,21 polymer based monoliths 22,23 and metal-organic frameworks. 24 Polymer-based monoliths are relatively easy to modify with functional groups.…”