Functionalized nanoparticles for liquid atmospheric pressure matrix‐assisted laser desorption/ionization peptide analysis

Turney, Kevin; Drake, Timothy J.; Smith, Joshua E.; Tan, Weihong; Harrison, W. W.

doi:10.1002/rcm.1634

Cited by 57 publications

(43 citation statements)

References 34 publications

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“…In addition, the interference of nanoparticles during the laser desorption/ionization process may not exist because of the smallness of the particles. Previously, C 18 functionalized silica nanoparticles have been utilized for hydrophobic interactions to extract peptides from acetonitrile, followed by liquid atmospheric pressure MALDI MS analysis [27]. Gold-modified magnetic nanoparticles [28] and diamond [29] were also used to selectively concentrate positively-charged proteins through electrostatic interaction.…”

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confidence: 99%

Analysis of peptides and proteins affinity-bound to iron oxide nanoparticles by MALDI MS

Chang

Zheng

Chen

et al. 2007

J. Am. Soc. Mass Spectrom.

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Iron oxide nanoparticles modified with oleate have been employed for the extraction of peptides and proteins from aqueous solution before matrix-assisted laser desorption/ionization (MALDI) mass spectrometric (MS) analysis. Adsorption of peptides and proteins onto the nanoparticles were mainly through electrostatic attraction and hydrophobic interaction. The analyte-adsorbed iron oxide nanoparticles could be efficiently collected from solution using a magnet. No elution step was needed. With this preconcentration strategy, the lowest detectable concentration of angiotensin I, insulin, and myoglobin in 500 L of aqueous solution were 0.1 nM, 0.1 nM, and 10.0 nM, respectively. In addition, the nanoparticles could extract the analytes from solution with a high content of salt and surfactant, thus eliminating suppression effect during MALDI MS analysis. This method was successfully applied to concentrate the tryptic digest products of cytochrome c. In addition, the tryptic digestion of cytochrome c can be directly conducted on the iron oxide nanoparticles. atrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) has become a routine analytical tool to determine the molecular mass of biomolecules [1][2][3]. However, samples containing excessive amounts of salts, surfactants, or other contaminations suffer from ionization suppression and adduct formation [4,5]. This limits the application of MALDI technique. Therefore, a simple and selective procedure for extraction and concentration of analyte from complex samples before MALDI MS is required.Various methods have been developed to isolate the analyte from complex sample matrix. In surface-enhanced laser desorption/ionization (SELDI), the sample target played an active role in the extraction, purification, or concentration of the analyte of interest [6 -9]. The target surface was derivatized for the selective retention of analyte while removing interferences through on-target washing. Several surface derivatizations have been designed to extract and concentrate the analyte through hydrophobic interaction [10,11], ionic interaction [12,13], or immunoaffinity [14,15]. However, the sensitivity improvement was limited by the number of binding sites on the target. In another approach, the so-called surface-enhanced affinity capture (SEAC), micrometer-sized beads made for chromatography column were used to capture peptides and proteins from sample solution [16]. Various types of beads have been used, which include reverse-phase chromatographic beads [17,18] and immobilized metal ion beads [19 -21]. To speed up the collection of analyteadsorbed beads from sample solution, magnetic particles, which can be simply collected using a magnet, were developed [22][23][24]. After collection, the microbeads were washed and placed on sample target, followed by analyzing with MALDI MS. Unfortunately, the presence of those particles on the sample target was reported to cause decrease in mass accuracy and resolution [25,26].Recently, nanoparticles have become interesting p...

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confidence: 99%

Analysis of peptides and proteins affinity-bound to iron oxide nanoparticles by MALDI MS

Chang

Zheng

Chen

et al. 2007

J. Am. Soc. Mass Spectrom.

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“…In fact, some outstanding features of NPs can be exploited to improve the analytical features of the MALDI technique. For instance, discrete NPs can be used for the isolation/preconcentration of analytes prior to MALDI analysis [59]. Interestingly, NPs seem also to offer important advantages over more conventional organic MALDI matrices, due to the fact that some NPs are excellent laser-energy-absorbing materials, their comparatively high surface area, ease of MALDI sample preparation and flexibility of the sample deposition [60].…”

Section: Matrix-assisted Laser Desorption/ionizationmentioning

confidence: 99%

Mass spectrometry for the characterization and quantification of engineered inorganic nanoparticles

Costa‐Fernández

Menéndez-Miranda

Bouzas-Ramos

et al. 2016

TrAC Trends in Analytical Chemistry

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A B S T R A C TAdvancements in nanoscience enabled the synthesis of a diverse array of engineered nanomaterials. The precise control of the composition and quality of such nanomaterials is required to ensure their eventual successful application. Also importantly, the potentially adverse environmental and/or human health effects resulting from exposure to nanoparticles (NPs) is still a critical but underexplored research area. To understand and assess the potentially harmful effects of NPs a proper knowledge of their physicochemical properties is required.Among the different available analytical tools for the characterization and quantification of engineered inorganic NPs, mass spectrometry (MS) offers outstanding capabilities. The analytical capabilities of different MS-based techniques, including elemental and molecular detection, and hybrid tools derived from their coupling with different separation approaches, to identify, characterize and quantify NPs is here revised. A forward look into trends on the use of MS for more complete chemical analysis of engineered inorganic NPs is finally attempted.

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“…Multi-step procedures, such as solid-phase extraction (SPE), are typically used for sample preparation but they do not remove analytes selectively [21]. In contrast, silica NPs with superparamagnetic properties have been developed as ideal media for the manipulation of trace amounts of bioanalytes from complex biological matrices in applications ranging from detection, separation to targeted delivery for hyperthermia treatment [15,22].…”

Section: Introductionmentioning

confidence: 99%

Bioconjugated silica nanoparticles: Development and applications

2008

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Advanced bioanalysis, including accurate quantitation, has driven the need to understand biology and medicine at the molecular level. Bioconjugated silica nanoparticles have the potential to address this emerging challenge. Particularly intriguing diagnostic and therapeutic applications in cancer and infectious disease as well as uses in gene and drug delivery, have also been found for silica nanoparticles. In this review, we describe the synthesis, bioconjugation, and applications of silica nanoparticles in different bioanalysis formats, such as selective tagging, barcoding, and separation of a wide range of biomedically important targets. Overall, we envisage that further development of these nanoparticles will provide a variety of advanced tools for molecular biology, genomics, proteomics and medicine.

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Functionalized nanoparticles for liquid atmospheric pressure matrix‐assisted laser desorption/ionization peptide analysis

Cited by 57 publications

References 34 publications

Analysis of peptides and proteins affinity-bound to iron oxide nanoparticles by MALDI MS

Analysis of peptides and proteins affinity-bound to iron oxide nanoparticles by MALDI MS

Mass spectrometry for the characterization and quantification of engineered inorganic nanoparticles

Bioconjugated silica nanoparticles: Development and applications

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