Functionally important residues of the Tet repressor inducing peptide TIP determined by a complete mutational analysis

A Novel TetR-Regulating Peptide Turns off rtTA-Mediated Activation of Gene Expression

“…A similar result was found for the TetR inducing peptides TIP and TIP2, since the crystal structures of both, [TetR⋅TIP] and [TetR⋅TIP2], revealed tryptophan residues localized within the tc-binding pocket forming crucial interactions to residues of TetR [28], [30]. For both peptides, the activity was lost when the tryptophan residues were exchanged to alanine [29], [31].…”

Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence

“…Increasing the inducer to TetR ratio is one promising way, since strains with less TetR, controlling the P xyl/tet promoter upstream of gfpmut2 , putatively respond better to lower levels of Tip‐tagged proteins ( Klotzsche et al ., 2005 ). To this end, S. aureus strains bearing autoregulated tetR , which generally ensures a balanced level of repressor molecules, might be exploited ( Gründling and Schneewind, 2007 ), optionally combined with improved TetR/Tip pairs ( Klotzsche et al ., 2005 ; Klotzsche et al ., 2007 ; Daam et al ., 2008 ). Taken together, proof‐of‐concept for a functional Tip‐tagging architecture in S. aureus was achieved using mCherry as a carrier protein.…”