Fundamental Points to Consider in the Cryopreservation and Shipment of Cells for Human Application

Stacey, Glyn; Healy, Lyn; Man, Jennifer; Hunt, Charles J.; Morris, John

doi:10.1002/9781118743362.ch6

Cited by 10 publications

(9 citation statements)

References 32 publications

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“…The severe cellular injuries that can occur during cooling are primarily a consequence of ice formation in the system, as noted above, and cooling rate is the key influencing factor over when and where ice forms. Having control over cooling rate provides a level of control over ice formation and, consequently, cell survival ( 6 , 10 , 11 , 107 ).…”

Section: The Freezing Processmentioning

confidence: 99%

“…As cytoplasmic concentration increases the probability of unavoidable, fatal, intracellular ice formation diminishes. If the applied cooling rate is too rapid, the extent of this dehydration is reduced, and the probability of intracellular ice formation increases ( 6 , 10 , 72 ). If the rate is too slow then dehydration may even become excessive and cause injury.…”

Section: The Freezing Processmentioning

confidence: 99%

“…Additional factors that can influence the positioning of the optimal cooling rate will include biological issues such as the type and origin the cell preparation, the cell density of the sample, cellular surface area to volume ratio and cell water permeability ( 6 , 7 , 147 , 148 ). Properties of the suspending medium, the properties of the cryoprotectants employed and any measures taken to induce ice nucleation will also influence the optimal cooling rate ( 6 , 7 , 10 , 73 ).…”

Section: The Freezing Processmentioning

confidence: 99%

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Cryopreservation as a Key Element in the Successful Delivery of Cell-Based Therapies—A Review

2020

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Cryopreservation is a key enabling technology in regenerative medicine that provides stable and secure extended cell storage for primary tissue isolates and constructs and prepared cell preparations. The essential detail of the process as it can be applied to cell-based therapies is set out in this review, covering tissue and cell isolation, cryoprotection, cooling and freezing, frozen storage and transport, thawing, and recovery. The aim is to provide clinical scientists with an overview of the benefits and difficulties associated with cryopreservation to assist them with problem resolution in their routine work, or to enable them to consider future involvement in cryopreservative procedures. It is also intended to facilitate networking between clinicians and cryo-researchers to review difficulties and problems to advance protocol optimization and innovative design.

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Section: The Freezing Processmentioning

confidence: 99%

Section: The Freezing Processmentioning

confidence: 99%

Section: The Freezing Processmentioning

confidence: 99%

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Cryopreservation as a Key Element in the Successful Delivery of Cell-Based Therapies—A Review

2020

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“…The challenges of preservation are influenced by the nature of individual cells such as their size, membrane permeability, morphology, ultrastructural complexity and composition of subcellular organelles, as well as the cell density and composition of the preservation medium (see reviews in [1][2][3]). A fundamental underlying factor to cryopreservation in any format is water stress (due to osmotic effects and ice formation), which the group believed to be still poorly understood, and requires further basic research These defining characteristics are complicated by the presence of multiple cell types or their combination with noncellular components, such as extracellular matrix or artificial scaffolds in certain therapies.…”

Section: Session 1: Categories Of Cell Systemsmentioning

confidence: 99%

Preservation and Stability of Cell Therapy Products: Recommendations from an Expert Workshop

et al. 2017

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If the field of regenerative medicine is to deliver therapies, rapid expansion and delivery over considerable distances to large numbers of patients is needed. This will demand efficient stabilization and shipment of cell products. However, cryopreservation science is poorly understood by life-scientists in general and in recent decades only limited progress has been made in the technology of preservation and storage of cells. Rapid translation of new developments to a broader range of cell types will be vital, as will assuring a deeper knowledge of the fundamental cell biology relating to successful preservation and recovery of cell cultures. This report presents expert consensus on these and other issues which need to be addressed for more efficient delivery of cell therapies.

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“…An equilibration phase is recommended upon CPA addition while considering temperature, concentration, and time to minimize toxicity (Andrews et al, 2015). For an explanation of the cryopreservation process see Stacey et al (2017).…”

Section: Cryoprotective Agent (Cpa)mentioning

confidence: 99%

Good Cell Culture Practice for stem cells and stem-cell-derived models

Pamies

2017

Toxicology Letters

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SummaryThe first guidance on Good Cell Culture Practice (GCCP) dates back to 2005. This document expands this to include aspects of quality assurance for in vitro cell culture focusing on the increasingly diverse cell types and culture formats used in research, product development, testing and manufacture of biotechnology products and cell-based medicines. It provides a set of basic principles of best practice that can be used in training new personnel, reviewing and improving local procedures, and helping to assure standard practices and conditions for the comparison of data between laboratories and experimentation performed at different times. This includes recommendations for the documentation and reporting of culture conditions. It is intended as guidance to facilitate the generation of reliable data from cell culture systems, and is not intended to conflict with local or higher level legislation or regulatory requirements. It may not be possible to meet all recommendations in this guidance for practical, legal or other reasons. However, when it is necessary to divert from the principles of GCCP, the risk of decreasing the quality of work and the safety of laboratory staff should be addressed and any conclusions or alternative approaches justified. This workshop report is considered a first step toward a revised GCCP 2.0.

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Fundamental Points to Consider in the Cryopreservation and Shipment of Cells for Human Application

Cited by 10 publications

References 32 publications

Cryopreservation as a Key Element in the Successful Delivery of Cell-Based Therapies—A Review

Cryopreservation as a Key Element in the Successful Delivery of Cell-Based Therapies—A Review

Preservation and Stability of Cell Therapy Products: Recommendations from an Expert Workshop

Good Cell Culture Practice for stem cells and stem-cell-derived models

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