Fundamental Study of Kitchen Refuse Utilization for Ethanol Fermentation by &lt;I&gt;Saccharomyces cerevisiae&lt;/I&gt;

Praneetrattananon, Suthasinee; Kiuchi, Takafumi; Dong, Ke; Wakisaka, Minato; Shirai, Yoshihito; Morimura, Shigeru; Kida, Kenji

doi:10.11301/jsfe2000.8.257

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…Thereafter, the kitchen refuse was again mixed in the following proportions: 43% (w/w) carbohydrates, 19% (w/w) protein and 38% (w/w) vegetables and fruits. The subsequent saccharification and pre-treatment for medium preparation were done as previously described (Praneetrattananon et al, 2007). The saccharified liquid was called 'kitchen refuse medium,' and was adjusted to various concentrations for inoculum preparation and fermentation.…”

Section: Preparation and Saccharification Of The Kitchen Refusementioning

confidence: 99%

“…Saccharomyces cerevisiae is the preferred yeast for most ethanol fermentation due to their high ethanol yield, high productivity and high ethanol tolerance (Lin and Tanaka, 2006). In previous study, the non-flocculating strain of S. cerevisiae ATCC 24860 was selected for ethanol production using kitchen refuse medium, a new potential biomass in Japan (Praneetrattananon et al, 2007). During successive cultivation, the strain steadily flocculated in kitchen refuse medium, but not in artificial medium under the same fermentation condition.…”

Section: Introductionmentioning

confidence: 99%

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Flocculation phenomenon of a mutant flocculent Saccharomyces cerevisiae strain: Effects of metal ions, sugars, temperature, pH, protein-denaturants and enzyme treatments

Ma¹,

Wakisaka²,

Sakai³

et al. 2010

Afr. J. Biotechnol.

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The flocculation mechanism of a stable mutant flocculent yeast strain Saccharomyces cerevisiae KRM-1 was quantitatively investigated for potential industrial interest. It was found that the mutant flocculent strain was NewFlo phenotype by means of sugar inhibition test. The flocculation was completely inhibited by treatment with proteinase K, protein-denaturants and carbohydrate modifier. The absence of calcium ions significantly inhibited the flocculation, indicating that Ca 2+ was specifically required for flocculation. The flocculation was stable when temperature below 70°C and pH was in the range of 3.0-6.0. The flocculation onset of the mutant flocculent strain was in the early stationary growth phase, which coincided with glucose depletion in the batch fermentation for the production of ethanol from kitchen refuse medium. The results are expected to help develop better strategies for the control of mutant flocculent yeast for future large-scale industrial ethanol fermentation.

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Section: Preparation and Saccharification Of The Kitchen Refusementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Flocculation phenomenon of a mutant flocculent Saccharomyces cerevisiae strain: Effects of metal ions, sugars, temperature, pH, protein-denaturants and enzyme treatments

Ma¹,

Wakisaka²,

Sakai³

et al. 2010

Afr. J. Biotechnol.

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Removal of Suspended Solid from Kitchen Garbage Saccharification Solution by Freezing and Thawing Technique

2009

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Freezing and thawing (FT) method has been known as an ef fective technique for removing suspended solid (SS) from suspensions including sewage sludge. In this study, saccharification solution produced from kitchen garbage has been chosen as sample solution, and FT technique has applied to the solution for the removal of SS in the solution. After the FT application for the saccharification solution (SS: 52700 ppm), SS in the solution have been reduced to 1300 ppm. And much clearer supernatant with lower SS content was obtained when FT solution kept at static condition overnight. Consequently, keeping FT solution at static condition overnight for SS sedimentation can remove over 99% of the SS from original kitchen garbage saccharification liquor. Saccharification liquor is easily damaged due to its characteristics. It should be kept frozen till its use as raw material for fermentation. Freezing and thawing offers good option both for SS removal and storage.

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Repeated-batch Ethanol Fermentation of Kitchen Refuse by Acid-tolerant Flocculating Yeast Under the Non-sterilized Condition

Dong

Wakisaka

Kiuchi³

et al. 2007

Japan J. Food Eng.

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The ethanol production performance of the flocculating yeast S. cerevisiae ATCC26602 from non-sterilized kitchen refuse medium in repeated-batch culturing was investigated. Although no pH modification or asepsis technology was applied, no contamination occurred. Twenty cycles of repeated-batch fermentation over a period of 11 days were successfully carried out without any loss of productivity by the self-flocculating strain ATCC26602. In addition, from the third batch, the flocculent strain achieved the reduction of the fermentation time to half, which resulted in an increasing of ethanol productivity to 3.7 g/L/h. These results of ethanol fermentation from kitchen refuse under the non-sterilized condition by this acid-tolerant and self-flocculation yeast may pave the way to low-cost ethanol production in pilot applications.

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Fundamental Study of Kitchen Refuse Utilization for Ethanol Fermentation by <I>Saccharomyces cerevisiae</I>

Cited by 3 publications

References 17 publications

Flocculation phenomenon of a mutant flocculent Saccharomyces cerevisiae strain: Effects of metal ions, sugars, temperature, pH, protein-denaturants and enzyme treatments

Flocculation phenomenon of a mutant flocculent Saccharomyces cerevisiae strain: Effects of metal ions, sugars, temperature, pH, protein-denaturants and enzyme treatments

Removal of Suspended Solid from Kitchen Garbage Saccharification Solution by Freezing and Thawing Technique

Repeated-batch Ethanol Fermentation of Kitchen Refuse by Acid-tolerant Flocculating Yeast Under the Non-sterilized Condition

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