Fungal Alcohol Dehydrogenases: Physiological Function, Molecular Properties, Regulation of Their Production, and Biotechnological Potential

Gutiérrez-Corona, J. Félix; González-Hernández, Gloria Angélica; Padilla-Guerrero, Israel Enrique; Olmedo-Monfil, Vianey; Martínez-Rocha, Ana Lilia; Patiño-Medina, J. Alberto; Meza-Carmen, Víctor; Torres-Guzmán, Juan Carlos

doi:10.3390/cells12182239

Cited by 7 publications

(3 citation statements)

References 167 publications

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“…This switch from respiration to fermentative metabolism to produce energy contributes to its survival in extreme cryoenvironments (Touchette et al ., 2022). In Mucor lusitanicus , a knock-out strain of the alcohol dehydrogenase ( adh1 ) gene, showed reduction in lipid and fatty acid content, which was restored by supplementing the media with external ethanol, suggesting that alcohol dehydrogenases are also involved in lipid biosynthesis (Gutiérrez-Corona et al ., 2023). The expansion of genes for alcohol dehydrogenases in psychrophiles might be involved either in the activation of alternative pathways for energy production, or in specific stages of lipid biosynthesis, which both could be alternatives to live in extreme cold environments.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics of the extremophileCryomyces antarcticusand other psychrophilic Dothideomycetes

Gomez,

Sic,

Haridas

et al. 2024

Preprint

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Cryomyces antarcticusis an endolithic fungus that inhabits rock outcrops in Antarctica. It survives extremes of cold, humidity and solar radiation in one of the least habitable environments on Earth. This fungus is unusual because it produces heavily melanized, meristematic growth and is thought to be haploid and asexual. Due to its growth in the most extreme environment, it has been suggested as an organism that could survive on Mars. However, the mechanisms it uses to achieve its extremophilic nature are not known. Over a billion years of fungal evolution has enabled representatives of this kingdom to populate almost all parts of planet Earth and to adapt to some of its most uninhabitable environments including extremes of temperature, salinity, pH, water, light, or other sources of radiation. Comparative genomics can provide clues to the processes underlying biological diversity, evolution, and adaptation. This effort has been greatly facilitated by the 1000 Fungal Genomes project and the JGI MycoCosm portal where sequenced genomes have been assembled into phylogenetic and ecological groups representing different projects, lifestyles, ecologies, and evolutionary histories. Comparative genomics within and between these groups provides insights into fungal adaptations, for example to extreme environmental conditions. Here, we analyze two Cryomyces genomes in the context of additional psychrophilic fungi, as well as non-psychrophilic fungi with diverse lifestyles selected from the MycoCosm database. This analysis identifies families of genes that are expanded and contracted in Cryomyces and other psychrophiles and may explain their extremophilic lifestyle. Higher GC contents of genes and of bases in the third positions of codons may help to stabilize DNA under extreme conditions. Numerous smaller contigs in C. antarcticus suggest the presence of an alternative haplotype that could indicate that the sequenced isolate is diploid or dikaryotic. These analyses provide a first step to unraveling the secrets of the extreme lifestyle ofC. antarcticus.

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Section: Discussionmentioning

confidence: 99%

Comparative genomics of the extremophileCryomyces antarcticusand other psychrophilic Dothideomycetes

Gomez,

Sic,

Haridas

et al. 2024

Preprint

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“…MDRs are involved in a variety of mechanisms, including growth and energy metabolism, under aerobic and anaerobic conditions [51]. Alcohol dehydrogenase catalyzes the interconversion of alcohols and aldehydes, which play an important role in the exposure of organisms to adverse stresses [15,52].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of the Medium-Chain Dehydrogenase/Reductase Superfamily of Trichosporon asahii and Its Involvement in the Regulation of Fluconazole Resistance

Ma,

Liu,

Zeng

et al. 2024

JoF

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The medium-chain dehydrogenase/reductase (MDR) superfamily contains many members that are widely present in organisms and play important roles in growth, metabolism, and stress resistance but have not been studied in Trichosporon asahii. In this study, bioinformatics and RNA sequencing methods were used to analyze the MDR superfamily of T. asahii and its regulatory effect on fluconazole resistance. A phylogenetic tree was constructed using Saccharomyces cerevisiae, Candida albicans, Cryptococcus neoformans, and T. asahii, and 73 MDRs were identified, all of which contained NADPH-binding motifs. T. asahii contained 20 MDRs that were unevenly distributed across six chromosomes. T. asahii MDRs (TaMDRs) had similar 3D structures but varied greatly in their genetic evolution at different phylum levels. RNA-seq and gene expression analyses revealed that the fluconazole-resistant T. asahii strain upregulates xylitol dehydrogenase, and downregulated alcohol dehydrogenase and sorbitol dehydrogenase concluded that the fluconazole-resistant T. asahii strain was less selective toward carbon sources and had higher adaptability to the environment. Overall, our study contributes to our understanding of TaMDRs, providing a basis for further analysis of the genes associated with drug resistance in T. asahii.

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“…It is commonly produced in many foods during the alcoholic fermentation of glucose by various microorganisms. The role of specific metabolic pathways of alcohol degradation and their role in fungal plant pathogens are not yet well understood [45].…”

Section: Discussionmentioning

confidence: 99%

Examination of Volatile Signatures of Fusarium Bulb Rot in Garlic Using Proton-Transfer-Reaction Time-of-Flight Mass Spectrometry and Solid-Phase Microextraction Gas Chromatography/Mass Spectrometry

Infantino,

Taiti,

Grottoli

et al. 2023

Separations

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Fusarium bulb rot, caused by Fusarium proliferatum, is a worldwide disease of garlic, both in the open field and during storage. Early diagnosis of the disease during storage is difficult due to the morphology of the bulbs and cloves. Volatile organic compounds (VOCs) are secondary metabolites produced by several microorganisms, including phytopathogenic fungi and bacteria. In recent years, the development of several techniques for the detection and characterization of VOCs has prompted their use, among others, as a diagnostic tool for the early and non-destructive analysis of many diseases of species of agricultural interest. In this paper, proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS) and solid-phase microextraction gas chromatography/mass spectrometry (SPME-GC/MS) were successfully utilized to characterize the volatolome of commercial garlic cloves, artificially and naturally infected with F. proliferatum, for the early discrimination between diseased and healthy ones. A partial least squares discriminant analysis (PLSDA) and a principal component analysis (PCA) allowed for the separation of infected and healthy cloves and the identification of specific VOCs produced by the fungus during the infection. The results obtained in this work could be utilized for the development of simpler, more economical, and more portable devices for the early detection of infected garlic bulbs during storage.

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Fungal Alcohol Dehydrogenases: Physiological Function, Molecular Properties, Regulation of Their Production, and Biotechnological Potential

Cited by 7 publications

References 167 publications

Comparative genomics of the extremophileCryomyces antarcticusand other psychrophilic Dothideomycetes

Comparative genomics of the extremophileCryomyces antarcticusand other psychrophilic Dothideomycetes

Genome-Wide Identification and Characterization of the Medium-Chain Dehydrogenase/Reductase Superfamily of Trichosporon asahii and Its Involvement in the Regulation of Fluconazole Resistance

Examination of Volatile Signatures of Fusarium Bulb Rot in Garlic Using Proton-Transfer-Reaction Time-of-Flight Mass Spectrometry and Solid-Phase Microextraction Gas Chromatography/Mass Spectrometry

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