Fungal Lipases: Versatile Tools for White Biotechnology

Pérez, Malena M.; Gonçalves, Enrico Cerioni Spiropulos; Vici, Ana Claudia; Salgado, José Carlos Santos; Polizeli, Maria de Lourdes Teixeira de Moraes

doi:10.1007/978-3-030-10480-1_11

Cited by 14 publications

(19 citation statements)

References 113 publications

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“…Hemachander and Puvanakrishnan (2000) also reported that the presence of detergents with a lipase produced by Ralstonia pickettii increased the effectiveness of removing stains by 24%-27% compared to its treatment with only detergents. Pérez et al (2019) demonstrate that biopolymers such as polyphenols, polysaccharides, and polyesters show a considerable degree of diversity and complexity. Actually, these biopolymers have become a major focus of researchers because they are biodegradable and are produced from renewable natural resources.…”

Section: Fungal Lipases In Detergent Industrymentioning

confidence: 99%

“…Fungal lipases are widely used in the leather Industry, since they are able to transform the natural fats present in the skins into free fatty acids and triacylglycerols (Pérez et al, 2019). These fungal enzymes are also used to help in the removal of size lubricants to provide the fabric with better absorbency for enhanced levelness of dyeing.…”

Section: Use Of Fungal Lipase In Textile Industrymentioning

confidence: 99%

“…In addition, the performance of fungal lipases as biocatalysts in the synthesis of esters and transesterification reactions in organic solvent systems allows the use of these lipolytic enzymes to catalyze the degradation of polyesters (Pérez et al, 2019).…”

Section: Production Of Biodegradable Polymersmentioning

confidence: 99%

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Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications

Mahfoudhi

Benmabrouk

Fendri

et al. 2022

Biotech & Bioengineering

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Many researchers have found fungi as a reliable source of lipase due to the versatility of their properties, ease of mass production, thermal stability, pH stability, broad substrate specificity, retained activity in organic solvents, and their low‐cost extraction procedure. This review paper presents an overview about the main aspects of fungal lipases screened from several types of strains as well as their use as biocatalysts. Additionally, some biochemical properties will be reported. As commonly known, lipases can be produced from animals, plants, and microorganisms. Compared to other lipases, those obtained from fungi have been found to be more productive, a fact that encouraged the massive production of most fungal lipases due to their considerable commercial importance during the past few years. This paper is concerned about some of the major characteristics that made fungal lipases desirable products in the industrial fields. Due to the enantioselective properties of fungal lipases and their ability to remain active under extreme temperature, pH, and organic solvents, enzymes are capable to synthesize esters as well as to catalyze a variety of chemical reactions that include esterification, transesterification, acidolysis and aminolysis in aqueous and nonaqueous media. Furthermore, lipases are considered to have a commercial importance for biotechnological application fields, which makes them increasingly popular in food, detergent, cosmetic, organic synthesis, and pharmaceutical domains. The biotechnological potential of lipases has made the latter a coveted choice in industries for the present and future as biocatalysts. In addition, a classification of these fungal enzymes is also highlighted in this review. Moreover, the impact of an immobilization strategy of these fungal strains to achieve higher yields and to improve their production is discussed. Finally, fungal enzymes have played a crucial role from ancient times to today in different fields using several types of biological systems, which gives them a great interest for the production of these enzymes in large amounts with low cost and easy viability to enlarge their use in many industries. Likewise, some future perspectives on lipase production will also be discussed by focusing on special cases on lipase engineering.

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Section: Fungal Lipases In Detergent Industrymentioning

confidence: 99%

Section: Use Of Fungal Lipase In Textile Industrymentioning

confidence: 99%

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Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications

Mahfoudhi

Benmabrouk

Fendri

et al. 2022

Biotech & Bioengineering

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“…Another typical element shared among lipases is a conserved motif of five amino acids (the pentapeptide) which contains the catalytic residue Ser (S). The most common pentapeptide is G-X-S-X-G, where X is whatever amino acid [ 4 ]. The majority of the lipases share this pentapeptide, reported in the PROSITE database as the PS00120 domain [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of a Novel Lipase with AHSMG Pentapeptide in Hypocreales and Glomerellales Filamentous Fungi

Gutiérrez-Domínguez

Chí-Manzanero

Rodríguez-Argüello

et al. 2022

IJMS

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Lipases are enzymes that hydrolyze triglycerides to fatty acids and glycerol. A typical element in lipases is a conserved motif of five amino acids (the pentapeptide), most commonly G-X-S-X-G. Lipases with the pentapeptide A-X-S-X-G are present in species of Bacillus, Paucimonas lemoignei, and the yeast Trichosporon asahii; they are usually thermotolerant and solvent resistant. Recently, while searching for true lipases in the Trichoderma harzianum genome, one lipase containing the pentapeptide AHSMG was identified. In this study, we cloned from T. harzianum strain B13-1 the lipase ID135964, renamed here as ThaL, which is 97.65% identical with the reference. We found that ThaL is a lid-containing true lipase of cluster III that belongs to a large family comprising highly conserved proteins in filamentous fungi in the orders Hypocreales and Glomerellales, in which predominantly pathogenic fungi are found. ThaL was expressed in conidia, as well as in T. harzianum mycelium, where it was cultured in liquid minimal medium. These results—together with the amino acid composition, absence of a signal peptide, mitochondrial sorting prediction, disordered regions in the protein, and lineage-specific phylogenetic distribution of its homologs—suggest that ThaL is a non-canonical effector. In summary, AHSMG-lipase is a novel lipase family in filamentous fungi, and is probably involved in pathogenicity.

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“…A relação entre concentração de substrato e velocidade inicial em (a) é sigmoide e em (b), hiperbólica. Fonte: Adaptado de PÉREZ et al (2018).…”

Section: Cinética De Lipasesunclassified

Estudos estruturais e funcionais de uma lipase de Beauveria bassiana imobilizada e expressa em Aspergillus nidulans: sensibilidade de linhagens celulares de glioblastoma aos hidrolisados de óleos brasileiros

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Fungal Lipases: Versatile Tools for White Biotechnology

Cited by 14 publications

References 113 publications

Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications

Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications

Identification of a Novel Lipase with AHSMG Pentapeptide in Hypocreales and Glomerellales Filamentous Fungi

Estudos estruturais e funcionais de uma lipase de Beauveria bassiana imobilizada e expressa em Aspergillus nidulans: sensibilidade de linhagens celulares de glioblastoma aos hidrolisados de óleos brasileiros

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