Anthracnose (Colletotrichum gloeosporioides) is the main disease affecting single assai palm seedlings and, therefore, a limiting factor to the production of quality seedlings. So far, there are no fungicide recommendations for the control of C. gloeosporioides in Euterpe precatoria. Thus, this study aimed to investigate the action of fungicides, such as pyraclostrobin + fluxapyroxad, as a possible alternative for controlling anthracnose in single assai palm seedlings. The experiment was installed in the nursery at Embrapa Acre, in a randomized block design, with seven treatments (0 – 0.155 – 0.187 – 0.218 – 0.250 – 0.281 – 0.312 μl of fungicide), with four replications of five plants. Evaluations were conducted for ten months regarding seedling height, stem diameter, number of leaves, shoot dry mass, root dry mass, total dry mass, and Dickson Quality Index. A visual scale ranging from 1 to 9 was used to assess the severity of the disease. The data were submitted to analysis of variance and regression analysis. Under the study conditions, the application of the active ingredient pyraclostrobin + fluxapyroxad reduced anthracnose severity by an average of 36.94% in single assai palm, providing better quality seedlings in the nursery.