Furoviruses (Virgaviridae)

Niehl, Annette; Koenig, R.

doi:10.1016/b978-0-12-809633-8.21312-5

Cited by 2 publications

(2 citation statements)

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“…Soil-borne wheat mosaic virus (SBWMV) and Soil-borne cereal mosaic virus (SBCMV), genus Furovirus, family Virgaviridae, are among the most devastating cereal viruses worldwide, leading on average to yield losses of 30-40% (Adams, 1990;Lapierre and Hariri, 2008;Kroese et al, 2020). SBWMV and SBCMV are positive sense single stranded bi-partite RNA viruses (King et al, 2011;Niehl and Koenig, 2021). RNA1 encodes a replication protein, an RNA-dependent RNA polymerase and a movement protein while RNA2 encodes a major coat protein (CP) and a minor CP, initiated by an upstream start codon, a CP-RT protein produced from translational readthrough of the stop codon and a cysteine rich silencing suppressor protein (Te et al, 2005;Andika et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…RNA1 encodes a replication protein, an RNA-dependent RNA polymerase and a movement protein while RNA2 encodes a major coat protein (CP) and a minor CP, initiated by an upstream start codon, a CP-RT protein produced from translational readthrough of the stop codon and a cysteine rich silencing suppressor protein (Te et al, 2005;Andika et al, 2016). Infections with SBWMV or SBCMV cause mosaic symptoms and light stunting, easily recognizable at field scale as chlorotic patches (Niehl and Koenig, 2021). The host range of SBWMV and SBCMV is relatively narrow, restricted to Poaceae, mostly cultivated, although infections of wild grasses of the genera Bromus were reported (McKinney, 1944;Lapierre and Signoret, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Nutrients and soil structure influence furovirus infection of wheat

et al. 2023

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Soil-borne wheat mosaic virus (SBWMV) and Soil-borne cereal mosaic virus (SBCMV), genus Furovirus, family Virgaviridae, cause significant crop losses in cereals. The viruses are transmitted by the soil-borne plasmodiophorid Polymyxa graminis. Inside P. graminis resting spores, the viruses persist in the soil for long time, which makes the disease difficult to combat. To open up novel possibilities for virus control, we explored the influence of physical and chemical soil properties on infection of wheat with SBWMV and SBCMV. Moreover, we investigated, whether infection rates are influenced by the nutritional state of the plants. Infection rates of susceptible wheat lines were correlated to soil structure parameters and nutrient contents in soil and plants. Our results show that SBWMV and SBCMV infection rates decrease the more water-impermeable the soil is and that virus transmission depends on pH. Moreover, we found that contents of several nutrients in the soil (e.g. phosphorous, magnesium, zinc) and in planta (e.g. nitrogen, carbon, boron, sulfur, calcium) affect SBWMV and SBCMV infection rates. The knowledge generated may help paving the way towards development of a microenvironment-adapted agriculture.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nutrients and soil structure influence furovirus infection of wheat

et al. 2023

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Detection and Quantification of Soil-Borne Wheat Mosaic Virus, Soil-Borne Cereal Mosaic Virus and Japanese Soil-Borne Wheat Mosaic Virus by ELISA and One-Step SYBR Green Real-Time Quantitative RT-PCR

Gauthier,

Strauch,

Bonse

et al. 2024

Viruses

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Furoviruses are bipartite viruses causing mosaic symptoms and stunting in cereals. Infection with these viruses can lead to severe crop losses. The virus species Furovirus tritici with soil-borne wheat mosaic virus (SBWMV), Furovirus cerealis with soil-borne cereal mosaic virus (SBCMV) and Furovirus japonicum with Japanese soil-borne wheat mosaic virus (JSBWMV) and French barley mosaic virus (FBMV) as members are biologically and genetically closely related. Here, we develop SYBR green-based real-time quantitative RT-PCR assays to detect and quantify the RNA1 and RNA2 of the three virus species. Using experimental data in combination with Tm-value prediction and analysis of primer and amplicon sequences, we determine the capacity of our method to discriminate between the different viruses and evaluate its genericity to detect different isolates within the same virus species. We demonstrate that our method is suitable for discriminating between the different virus species and allows for the detection of different virus isolates. However, JSBWMV RNA1 primers may amplify SBWMV samples, bearing a risk for false positive detection with this primer. We also test the efficiency of polyclonal antibodies to detect the different viruses by ELISA and suggest that ELISA may be applied as a first screening to identify the virus. The real-time qRT-PCR assays developed provide the possibility to screen for quantitative disease resistance against SBCMV, SBWMV and JSBWMV. Moreover, with our method, we hope to promote research to unravel yet unresolved questions with respect to furovirus–host interaction concerning host range and resistance as well as regarding the role of multipartite genomes.

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Furoviruses (Virgaviridae)

Cited by 2 publications

References 20 publications

Nutrients and soil structure influence furovirus infection of wheat

Nutrients and soil structure influence furovirus infection of wheat

Detection and Quantification of Soil-Borne Wheat Mosaic Virus, Soil-Borne Cereal Mosaic Virus and Japanese Soil-Borne Wheat Mosaic Virus by ELISA and One-Step SYBR Green Real-Time Quantitative RT-PCR

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