Further characterization of 41 isolates of adenovirus types 19/37 by serum neutralization and DNA restriction enzyme analysis

Abstract

SUMMARYForty-one strains of adenovirus type 19/37 (Adl9/37) mainly isolated from patients with keratoconjunctivitis or conjunctivitis between 1974 and 1984 were re-evaluated by serum neutralization (SN), haemagglutination inhibition (HI) and DNA restriction analysis. Of 19 isolates which were neutralized to high titre by antiserum prepared against prototype Adl9, 5 showed cross-reactivity with 32-64 units of Ad37 antiserum, while of 22 strains neutralized to high titre by Ad37 antiserum, 3 showed cross-reactivity with 32 units of Adl9 antiserum. By DNA restriction analysis, all Adl9 isolates were identical to each other and to Adl9A virus. Using endonuclease Bgl 1, three variants were observed among the Ad37 isolates.

Five New Genome Types of Adenovirus Type 37 Caused Epidemic Keratoconjunctivitis in Sapporo, Japan, for More Than 10 Years

“…It has already been reported that the genetic variability of HAdV-37 is smaller than that of other adenovirus serotypes (2,21). The restriction analysis of several HAdV-37 strains isolated between 1976 and 1983 in Europe, Australia, the United States, and Japan showed that HAdV-37 variants shared more than 90% of their restriction fragments (2,17).…”

“…After HAdV-37 was initially characterized (33), many reports appeared about variations in the HAdV-37 genome isolated from patients with ocular and urethral diseases (2,13,14,21,22,26). Reports in the 1980s showed that the genome of HAdV-37 strains seemed to be rather stable (2,14,21). In Sapporo, the genome type of HAdV-37 isolates was almost all HAdV-37p (4, 13, 14) until 1988.…”

Human Adenovirus from Subgenus D: Restriction Mapping of Types 9 and 19 and Characterization of a New Genome Type

“…Wadell & de Jong (1980) have identified a genome type of AV 19 responsible for outbreaks of keratoconjunctivitis of which the DNA restriction pattern was different from AV19. This genome type, named AV19a for distinction from the prototype AV 19p, has been isolated from many patients with keratoconjunctivitis (Kemp et al, 1983;Meng et al, 1986). BamHI, BglII and SmaI restriction patterns of strain 208 were clearly different from those published for both AV 19a and AV 19p.…”

“…For typing clinical isolates of AV, Fife et al (1985) have compared neutralization and restriction enzyme methods and shown that the latter provided the best results. This method has been used to characterize clinical isolates, variants and intermediate AV strains (Adrian et al, 1985 a;Hammond et al, 1985 ;Kemp & Hierholzer, 1986, Meng et al, 1986Niel et al, 1986;Notzel et al, 1985). Wadell & de Jong (1980) have identified a genome type of AV 19 responsible for outbreaks of keratoconjunctivitis of which the DNA restriction pattern was different from AV19.…”

Genome Typing of Adenovirus Strains Isolated from Conjunctivitis in Japan, Australia, and the Philippines

“…Ad37, which is also the member of subgroup D, was first identified in 1976 (9). This virus is also associated with keratoconjunctivitis (3,4,9,16,19,23,26,37). Ad4, the only member of subgroup E, was isolated from children in Europe and in the United States (37), and is also a causative agent of keratoconjunctivitis (2,4,5,11,21,24,25,37).…”