Further characterization of a cattle nematode population with demonstrated resistance to current anthelmintics

Gasbarre, Louis C.; Smith, Larry L.; Hoberg, Eric P.; Pilitt, P. A.

doi:10.1016/j.vetpar.2009.08.019

Cited by 62 publications

(32 citation statements)

References 14 publications

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“…These results suggested that F200Y (T A C) resistance mutation is likely to be present in many H. placei populations of buffalo and cattle. Previously benzimidazole resistance has been reported in H. placei in Brazil (Bricarello et al, 2007), Argentina (Anziani et al, 2004), USA (Gasbarre et al, 2009b; Gasbarre et al, 2009a) and north India (Yadav and Verma, 1997), but there is little information on its prevalence. These reports are based on faecal egg count reduction tests (FECRTs) to give an estimate of the benzimidazole resistance or susceptibility status of a parasite community as a whole, but cannot be used to assess the proportion of benzimidazole resistance worms in a particular population (Achi et al, 2003; Jacquiet et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Emergence and the spread of the F200Y benzimidazole resistance mutation in Haemonchus contortus and Haemonchus placei from buffalo and cattle

Ali¹,

Rashid²,

Shabbir

et al. 2018

Preprint

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9Benzimidazoles have been intensively used in the livestock sector, particularly in small 3 0 ruminants for over 40 years. This has been led to the widespread emergence of resistance in a 3 1 number of small ruminant parasite species, in particular Haemonchus contortus. In many 3 2 counties benzimidazole resistance in the small ruminants H. contortus has become severely 3 3 compromising its control; but there is a little information on benzimidazole resistance in H. 3 4 contortus infecting buffalo and cattle. Resistance to benzimidazoles have also been reported 3 5 in the large ruminant parasite, Haemonchus placei, but again there is relatively little 3 6 information on its prevalence. Hence it is extremely important to understand how resistance-3 7 conferring mutations emerge and spread in both parasites in the buffalo and cattle host in 3 8 order to develop the approaches for the recognition of the problem at an early stage of its 3 9 development. The present study suggests that the F200Y (TAC) mutation is common in H. 4 0 contortus, being detected in 5/7 populations at frequencies between 7 to 57%. Furthermore, 4 1 6/10 H. placei populations contained the F200Y (TAC) mutation, albeit at low frequencies of 4 2 between 0.4 to 5%. The phylogenetic analysis suggests that the F200Y (TAC) mutation in H. 4 3 contortus has emerged on multiple occasions in the region, with at least three independent 4 4 emergence of resistance alleles across the populations. In contrast, the F200Y (TAC) 4 5 resistance-conferring mutation in H. placei is only seen on a single haplotype. A high level of 4 6 haplotype frequency of the susceptible alleles in the region, suggests that the unique 4 7 resistance conferring-mutation has spread from a single emergence; likely by anthropogenic 4 8 animal movement. Overall, these results provide the first clear genetic evidence for the 4 9Recently, we have developed and validated the Illumina Mi-seq deep amplicon sequencing

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Section: Discussionmentioning

confidence: 99%

Emergence and the spread of the F200Y benzimidazole resistance mutation in Haemonchus contortus and Haemonchus placei from buffalo and cattle

Ali¹,

Rashid²,

Shabbir

et al. 2018

Preprint

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“…are important, highly pathogenic blood feeding parasites of cattle in many parts of the world, particular in warmer regions with high humidity, such as Asia, south America and the south- ern USA. H. placei is the most common species traditionally reported from cattle (Gasbarre et al, 2009a;Gasbarre et al, 2009b). Of the four populations examined (one from Sargodha, one from Lahore, one from Okara and one from Sahiwal), the prevalence of 85% H. placei suggesting this is still the predominant Haemonchus species infecting cattle in Pakistan.…”

Section: Journal Of Infection and Molecularmentioning

confidence: 94%

Genetic Variation in the rDNA ITS-2 Sequence of Haemonchus placei from Cattle Host

Ali¹

2015

J. Inf. Mol. Biol.

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“…The general classes of compounds, called anthelmintics, have been developed to treat infections by parasitic nematodes ( 13 , 21 , 27 ). One class of anthelmintics approved for use by WHO is the nicotinic acetylcholine receptor (nAChR) agonist, which can be further divided into two targeted receptor subtypes: L-type and N-type ( 27 ).…”

Section: Introductionmentioning

confidence: 99%

Effective drug combination for Caenorhabditis elegans nematodes discovered by output-driven feedback system control technique

Njus

Kong

et al. 2017

Sci. Adv.

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Further characterization of a cattle nematode population with demonstrated resistance to current anthelmintics

Cited by 62 publications

References 14 publications

Emergence and the spread of the F200Y benzimidazole resistance mutation in Haemonchus contortus and Haemonchus placei from buffalo and cattle

Emergence and the spread of the F200Y benzimidazole resistance mutation in Haemonchus contortus and Haemonchus placei from buffalo and cattle

Genetic Variation in the rDNA ITS-2 Sequence of Haemonchus placei from Cattle Host

Effective drug combination for Caenorhabditis elegans nematodes discovered by output-driven feedback system control technique

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