Further Evidence for Glutenin Modifications in Winter Wheat (<i>Triticum Aestivum</i>L.) Induced by Somaclonal Variation

Beshkova, N.; Ivanov, P.; Ivanova, I.

doi:10.1080/13102818.1998.10818988

Cited by 7 publications

(5 citation statements)

References 7 publications

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“…It could be effectively used as a marker for discrimination not only of Pryaspa and Pryaspa 1 but also of Pryaspa 1 from the observed biotypes of Yantar. The reduction of protein content corresponding to subunit 7 encoded by Glu-B1 locus was not observed in the other varieties included in this study, but has been described in previous studies (4).…”

Section: Resultsmentioning

confidence: 62%

Assessing the Genetic Variation of Bulgarian Bread Wheat Varieties by Biochemical and Molecular Markers

Zheleva

Todorovska

Christov

et al. 2007

Biotechnology & Biotechnological Equipment

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Section: Resultsmentioning

confidence: 62%

Assessing the Genetic Variation of Bulgarian Bread Wheat Varieties by Biochemical and Molecular Markers

Zheleva

Todorovska

Christov

et al. 2007

Biotechnology & Biotechnological Equipment

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“…Deletion of HMW-GSs results from gene silencing. The gene silencing of HMW-GSs could be caused by the deletion of small chromosomal fragments and the alteration of promoter regions ( Upelniek et al, 1995 ; Beshkova et al, 1998 ; Liu et al, 2016 ). Single base substitutions in the coding region sequences also cause gene silencing in the deletion of HMW-GSs in wheat, which usually results from premature termination codons ( Chen et al, 2019 , 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Premature Termination Codon of 1Dy12 Gene Improves Cookie Quality in Ningmai9 Wheat

et al. 2022

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The area between middle and lower reaches of the Yangtze River is the largest region for soft wheat production in China. In soft wheat breeding, the lack of germplasm with desirable quality for end-use products is a barrier. Ningmai9 is the main variety of soft wheat planted in this area. To create germplasm with better quality and yield potential than Ningmai9, mutants of HMW-GSs in Ningmai9 induced by ethylmethanesulfonate (EMS) were obtained. SDS-PAGE showed that two mutants, md10 and md11, were HMW-GS 1Dy deletions. DNA sequencing confirmed that one mutation was caused by a C/T substitution, resulting in the change of CAA encoding glutamine into the termination codon TAA, and another mutation was due to a G/A substitution in the central repetitive domain of the coding region, causing TGG encoding tryptophan to become the termination codon TGA. The premature termination codon of the 1Dy12 gene affected the expression of 1Dy12 and kept the mRNA at a lower transcription level during the kernel development stage in comparison with the wild type. HMW-GS 1Dy12 deletion mutants decreased the content of HMW-GSs and glutenin macropolymers, mixograph envelope peak time and TIMEX width, water solvent retention capacity (WSRC), and lactic acid solvent retention capacity (LASRC). In the HMW-GS 1Dy12 deletion lines, the sugar-snap cookie diameter was 8.70–8.74 cm, which was significantly larger than that in the wild type of 8.0 cm. There were no significant differences in spike number, kernel number, thousand kernel weight, and yield between the deletion lines and wild type. Overall, the study indicated that the knockout of the HMW-GS gene induced by EMS is an effective way to improve wheat quality, and deletion mutants of HMW-GS 1Dy12 decrease gluten strength and increase sugar snap cookie diameter without yield penalty in Ningmai9 wheat.

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“…Tissue culture can be used to induce variation to wheat storage protein alleles [ 58 – 59 ]. In regenerated plants from the immature embryo cultures of the common wheat line CS, the variation for one protein band and protein bands controlled by the locus, mostly for gliadins, were observed with frequencies of 0.11% and 0.69%, respectively [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

“…In regenerated plants from the immature embryo cultures of the common wheat line CS, the variation for one protein band and protein bands controlled by the locus, mostly for gliadins, were observed with frequencies of 0.11% and 0.69%, respectively [ 58 ]. In the regeneration offspring from in vitro culture of immature embryos of four winter varieties, one plant lacking 1Bx7 and 11By9 at the Glu-B1 locus was found, and alteration at this locus with decreased expression occurred in only one wheat genotype [ 59 ]. In this study, we reported a somatic variation mutant AS208 lacking genes encoding 1Bx20 and 1By20 that was identified from the tissue culture offspring of a commercial winter variety, LX987.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, we suggested that the 1Bx20 and 1Bx20y in AS208 was deleted from its genome led to their slicing in AS208. In previous reports, the silencing of genes encoding storage proteins was suggested to be caused by chromosomal deletion and promoter region alterations [ 58 – 59 ]. The silencing mechanism of the 1Bx20 and 1Bx20y in AS208 needs to be investigated further at the molecular level.…”

Section: Discussionmentioning

confidence: 99%

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Comprehensive Identification and Bread-Making Quality Evaluation of Common Wheat Somatic Variation Line AS208 on Glutenin Composition

et al. 2016

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High molecular weight glutenin subunits (HMW-GSs) are important seed storage proteins in wheat (Triticum aestivum) that determine wheat dough elasticity and processing quality. Clarification of the defined effectiveness of HMW-GSs is very important to breeding efforts aimed at improving wheat quality. To date, there have no report on the expression silencing and quality effects of 1Bx20 and 1By20 at the Glu-B1 locus in wheat. A wheat somatic variation line, AS208, in which both 1Bx20 and 1By20 at Glu-B1 locus were silenced, was developed recently in our laboratory. Evaluation of agronomic traits and seed storage proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed-phase high performance liquid chromatography (RP-HPLC) indicated that AS208 was highly similar to its parental cultivar Lunxuan987 (LX987), with the exception that the composition and expression of HMW-GSs was altered. The 1Bx20 and 1By20 in AS208 were further identified to be missing by polymerase chain reaction (PCR) and quantitative real-time RT-PCR (qRT-PCR) assays. Based on the PCR results for HMW-GS genes and their promoters in AS208 compared with LX987, 1Bx20 and 1By20 were speculated to be deleted in AS208 during in vitro culture. Quality analysis of this line with Mixograph, Farinograph, and Extensograph instruments, as well as analysis of bread-making quality traits, demonstrated that the lack of the genes encoding 1Bx20 and 1By20 caused various negative effects on dough processing and bread-making quality traits, including falling number, dough stability time, mixing tolerance index, crude protein values, wet gluten content, bread size, and internal cell structure. AS208 can potentially be used in the functional dissection of other HMW-GSs as a plant material with desirable genetic background, and in biscuit making industry as a high-quality weak gluten wheat source.

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Further Evidence for Glutenin Modifications in Winter Wheat (Triticum AestivumL.) Induced by Somaclonal Variation

Abstract: The high molecular weight (HlvfW)

Cited by 7 publications

References 7 publications

Assessing the Genetic Variation of Bulgarian Bread Wheat Varieties by Biochemical and Molecular Markers

Assessing the Genetic Variation of Bulgarian Bread Wheat Varieties by Biochemical and Molecular Markers

Premature Termination Codon of 1Dy12 Gene Improves Cookie Quality in Ningmai9 Wheat

Comprehensive Identification and Bread-Making Quality Evaluation of Common Wheat Somatic Variation Line AS208 on Glutenin Composition

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