Summary Male meiosis and pollen fertility studies have been performed in 35 species belonging to 25 genera and 13 families of Polypetalae from the Dalhousie hills. Impatiens laxiflora (nϭ6) was counted chromosomally for the first time. Corydalis cornuta (nϭ8), Erysimum hieracifolum (nϭ16), Arenaria serpyllifolia (nϭ10), Drymaria diandra (nϭ13), Geranium lucidum (nϭ14), Nasturtium officinale (nϭ16), Geum roylei (nϭ21) and Chaerophyllum reflexum (nϭ11) are worked out chromosomally for the first time in India. New cytotypes are recorded for Thalictrum saniculiforme (nϭ14), Drymaria diandra (nϭ13), Geranium lucidum (nϭ14) and Chaerophyllum reflexum (nϭ11). Present chromosome counts for the rest of the species substantiate earlier reports by other researchers. Intraspecific diploid (nϭ12) and tetraploid (nϭ24) cytotypes recorded presently from Dalhousie hills in Oxalis corniculata are morphologically indistinguishable. The phenomenon of cytomixis involving inter PMC (pollen mother cell) migration of chromatin material has been observed in Ranunculus diffusus (nϭ16), Thalictrum foliolosum (nϭ7), T. saniculiforme (nϭ14), Gypsophila cerastioides (nϭ15), Oxalis corniculata (nϭ24), Murraya koenigii (nϭ9), Geum roylei (nϭ21), Potentilla atrisanguinea (nϭ21) and Pimpinella diversifolia (nϭ9). Cytomixis in these species results in various meiotic abnormalities and heterogeneous sized pollen grains. In the remaining species, the meiosis is normal, leading to high pollen fertility.