Further Klebsiella Capsule Types

Edmunds, P. N.

doi:10.1093/infdis/94.1.65

Cited by 32 publications

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“…Any new scheme of classification should be compared with the old; our scheme supplements rather than replaces those based on serology (Julianelle, 1926 ;Kauffmann, 1949 Edmunds, 1954;IBrskov, 1954IBrskov, , 1955. It provides a means of characterizing species within the genus and, we hope, will help to bring classification of the Enterobacteriaceae into line with other groups of bacteria in which genera are split into species which in turn are subdivided into serotypes or other subspecific taxa.…”

Section: Discussionmentioning

confidence: 99%

A Classification of the Klebsiella Group

Cowan

Steel

Shaw

et al. 1960

Journal of General Microbiology

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SUMMARYKlebsiella strains are divided into six categories on the following reactions: fimbriation, production of gas from glucose a t 37", acid from lactose and dulcitol, methyl red test, acetoin production, citrate, urease, gluconate, malonate, lysine decarboxylase, growth in the presence of KCN and ability to produce gas from lactose a t 44". Five of the six categories are assigned specific rank, the sixth subspecific rank. The tests enable Friedlander's pneumobacillus to be distinguished from Aerobacter aerogenes. A new species, Klebsiella edwardsii, and a new subspecies, K . edwardsii var. atlantae, are named. Neotype strains of old species are proposed, holotypes of the new species and variety designated, and the catalogue numbers in the National Collection of Type Cultures and American Type Culture Collection given.

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Section: Discussionmentioning

confidence: 99%

A Classification of the Klebsiella Group

Cowan

Steel

Shaw

et al. 1960

Journal of General Microbiology

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“…A second capsulated-noncapsulated strain pair [264(1) and 264(1)NCV] was also employed. K. pneumoniae strain 264(1) is of serotype O7:K67 (10). A noncapsulated version, 264(1)NCV, was isolated essentially as previously described (46) and does not react with anti-K67 serum.…”

Section: Methodsmentioning

confidence: 99%

Capsule and Fimbria Interaction inKlebsiella pneumoniae

Blom²,

et al. 2005

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The capsular polysaccharide and type 1 fimbriae are two of the major surface-located virulence properties associated with the pathogenesis of Klebsiella pneumoniae. The capsule is an elaborate polysaccharide matrix that encases the entire cell surface and provides resistance against many host defense mechanisms. In contrast, type 1 fimbriae are thin adhesive thread-like surface organelles that can extend beyond the capsular matrix and mediate D-mannose-sensitive adhesion to host epithelial cells. These fimbriae are archetypical and consist of a major building block protein (FimA) that comprises the bulk of the organelle and a tip-located adhesin (FimH). It is assumed that the extended major-subunit protein structure permits the FimH adhesin to function independently of the presence of a capsule. In this study, we have employed a defined set of K. pneumoniae capsulated and noncapsulated strains to show that the function of type 1 fimbriae is actually impeded by the concomitant expression of a polysaccharide capsule. Capsule expression had significant effects on two parameters commonly used to define FimH function, namely, yeast cell agglutination and biofilm formation. Our data suggest that this effect is not due to transcriptional/translational changes in fimbrial gene/protein expression but rather the result of direct physical interference. This was further demonstrated by the fact that we could restore fimbrial function by inhibiting capsule synthesis. It remains to be determined whether the expression of these very different surface components occurs simply via random events of phase variation or in a coordinated manner in response to specific environmental cues.

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“…When tested with an antiserum prepared for the typical gelatin-liquefying A . cloacae strain US (described by Edmunds, 1954) the slimes of strains NCTC 5920 and 5936 both gave precipitation to the significant titre (Ills).…”

Section: Aerobacter Aerogenes Strain B 301 ( R )mentioning

confidence: 99%

The Distribution of Polysaccharide Production in Aerobacter and Escherichia Strains and its relation to Antigenic Character:With a note on the Influence of Potassium Deficiency upon Production of Polysaccharide by Aerobacter aerogenes

Edmunds

Duguid

Wilkinson

1954

Journal of General Microbiology

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SUMMARY: Strains of Aerobacter aerogenes, A. cloacae and Escherichia coli, includingmucoid capsulate, mucoid non-capsulate, smooth and rough variant forms, were grown on defined agar media. The polysaccharide content of the cultures was estimated by the anthrone method and related to the amount of growth as measured by the non-dialysable nitrogen content. Polysaccharide production, as measured by the polysaccharide : nitrogen ratio, was increased in all strains when growth was limited by a restricted supply of nitrogen source so that added carbohydrate remained in relative excess. A low incubation temperature, 15-20° instead of 3 5 O , influenced polysaccharide production differently in the various strains and species ; thus it greatly increased production in E. coli strains, but decreased it in A. cloacae strains grown on certain media. The various strains showed considerable differences in the amount of polysaccharide production and in the distribution of the polysaccharide between the different possible sites of accumulation : i.e. intracellular, capsular and as loose slime. The mucoid strains produced the largest amounts of polysaccharide, most of which was present extracellularly as capsuIes or slime; the smooth strains produced much smaller amounts, almost entirely intracellular; a rough strain produced least of all. The antigenic constitution of certain strains was examined, particularly that of a mucoid capsulate A. aerogenes and mucoid non-capsulate and non-mucoid smooth variants derived from it. The designation of such morphological and immunological variants is discussed.The amount of polysaccharide produced varies greatly with different strains and species of Enterobacteriaceae. Among twelve strains of Escherichia coli grown at 13-17' on glucose medium, Morgan & Beckwith (1939) found that the amount of polysaccharide produced, as indicated by the degree of mucoidness, was large in five strains, moderate in two strains and small in the remainder ; they found similar differences among salmonella strains. Mucoid, smooth and rough variants of the same strain may also show great differences in polysaccharide production. (1951), using the periodate-Schiff staining method, found that 0-inagglutinable rough variants of salmonella and shigella strains lacked the stainable intracellular polysaccharide of the smooth parent strains ; they therefore suggested that this polysaccharide corresponded to the hapten of the 0 antigen characteristic of the smooth form.Duguid & Wilkinson (1953) studied the influence of different cultural conditions on the amount of polysaccharide produced by a single strain (A3) of Aerobacter aerogenes, distinguishing the polysaccharide situated intracellularly, in the capsules or in the loose extracellular slime. In the present investigation the same methods have been applied for examination of several different strains of A . aerogenes, A . cloacae and Eschrichia coli. For each strain the effect was observed of variation in the sugar:nitrogen ratio of the medium and of variation in th...

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Further Klebsiella Capsule Types

Cited by 32 publications

References 0 publications

A Classification of the Klebsiella Group

A Classification of the Klebsiella Group

Capsule and Fimbria Interaction inKlebsiella pneumoniae

The Distribution of Polysaccharide Production in Aerobacter and Escherichia Strains and its relation to Antigenic Character:With a note on the Influence of Potassium Deficiency upon Production of Polysaccharide by Aerobacter aerogenes

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