Further research on the biological function of inclusion bodies ofAnomala cupreaentomopoxvirus, with special reference to the effect on the insecticidal activity of aBacillus thuringiensisformulation

Abstract

The results suggest that spindles or the constituent protein fusolin can be used as a coagent with Bt formulations, and that fusolin coexpression with a Bt toxin in crops might improve the insecticidal efficacy. In addition, the spheroids are potential biocontrol agents for some scarabaeid pests that are not easy to control because of their underground habitation.

Genes for Membrane Transport Proteins: Not So Rare in Viruses

“…The insect viruses Anomala cuprea entomopoxvirus (ACEV) and Amsacta moorei entomopoxvirus (AMEV) are the only viruses that carry a gene coding for a large ABC transporter. In the ACEV genome ORF ACV025 is the largest ORF coding for a protein of 1506 aa ([ 50 ]; accession #YP_009001498.1). The most similar proteins are from beetles (e.g., the ATP-binding cassette sub-family A member 3-like isoform X1 from Onthophagus taurus , blastp E value 0; 34% aa identity; XP_022911124.1); the high degree of similarity suggests that the gene was only recently derived from the host ( Figure 6 ).…”

A support vector machine approach to identification of proteins relevant to learning in a mouse model of Down Syndrome

“…Adults are folivores and are active at night (Fujiyama et al, 1979). Recorded larval hosts include sugarcane (Saccharum officinarum) (Mitsuhashi et al, 2014), beans (Bhuiyan and Nishigaki, 1996), turf grass (Suzuki et al, 1994), sweet potato (Ipomoea batatas) (Suzuki et al, 1994), and peanuts (Arachis hypogaea) (Kato et al, 1980). (Fujiyama et al, 1979): Adults are active June through September (Kobayashi and Matsumoto, 2011), with a single generation per year.…”

Search for genes gained by horizontal gene transfer in an entomopoxvirus, with special reference to the analysis of the transfer of an ABC transporter gene

“…The A. cuprea larvae used were offspring of adults collected in fields in the Kanto district, Japan, and reared till their use according to the methods of Mitsuhashi et al (2014a) . We used ACEV strain CV6M ( Mitsuhashi et al, 2014b ), which was propagated in A. cuprea larvae in our laboratory according to the method of Mitsuhashi et al (2014a) , for the present experiments.…”

“…For inoculation to A. cuprea for RT-PCR of an ABC transporter gene, inclusion bodies (a mixture of spheroids and spindles) were partially purified from dead A. cuprea larvae infected with the ACEV according to the method of Mitsuhashi et al (2014a) , with the exception that sucrose density gradient centrifugation was omitted.…”