Further studies on the effects of centrophenoxine on lipofuscin pigment in neuroblastoma cells in culture: An electron microscopic study

Nandy, Kalidas; Baste, Claudia A.; Schneider, F. Howard

doi:10.1016/0531-5565(78)90040-2

Cited by 16 publications

(2 citation statements)

References 15 publications

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“…Zs-Nagy et al [21] also reported that CpH supplementation produced marked improvement in learning, memory, and mental alertness. Similarly, Nandy [22] reported a significant improvement of symptoms such as fatigue, irritability, confusional states, and loss of memory in the geriatric patient treated with CpH. According to his studies CpH-treated mice did better on learning tasks as compared to control mice.…”

Section: Discussionmentioning

confidence: 90%

Biochemical alterations in rat brain following aluminum exposure: Protection with centrophenoxine

Nehru

Bhalla

2007

Toxicological & Environmental Chemistry

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Aluminum (Al) is a neurotoxicant potentially affecting ionic, cholinergic, and dopaminergic neurotransmission in the central nervous system. These alterations are known to be associated with learning ability, adaptive responses, and other aspects of behavior. The present experiment was designed to study the neurotoxic consequences of Al exposure on neurotransmitters like dopamine (DA), serotonin (5-HT), norepinephrine (NE) along with the activity of acetylcholinesterase (AChE). Furthermore, Centrophenoxine (CpH) was administered as a post treatment to evaluate its potential in Al-induced neurotoxicity. The cognitive functions and memory loss were also studied after both Al and CpH administration. Al was administered orally at a dose of 40 mg kg À1 day À1 for a period of 8 weeks, whereas CpH was administered intraperitoneally at a dose of 100 À1 mg kg À1 day À1 for a period of 6 weeks. The study was carried out in four regions of the brain, namely cerebrum, cerebellum, medulla oblongata, and hypothalamus. A significant reduction in AChE activity and different neurotransmitters was observed after Al exposure in the regions. CpH as a post treatment proved beneficial in restoring these alterations. Al exposure also affected the cognitive functions and short-term memory, which were significantly improved following CpH post treatment.

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Section: Discussionmentioning

confidence: 90%

Biochemical alterations in rat brain following aluminum exposure: Protection with centrophenoxine

Nehru

Bhalla

2007

Toxicological & Environmental Chemistry

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“…In the study of lipofuscin, the cell culture system has been regarded as a useful model. Cultured cells such as neurons [8][9][10], glial cells [11,12], neuroblastoma cells [13][14][15], cardiac myocytes [7,[16][17][18][19] and fibroblasts [20] are known to accumulate lipofuscin within a short period. In our laboratory, we have tested some cell lines to study the mechanism of lipofuscin formation in neurons and found that NG108-15 cells cease proliferation and accumulate lipofuscin-like autofluorescent materials during neuronal differentiation.…”

Section: Introductionmentioning

confidence: 99%

Formation of Lipofuscin-Like Autofluorescent Materials in NG108-15 Cells: Involvement of Lysosomal Protein Degradation

Mochizuki

Park²,

Mori³

et al. 1997

Gerontology

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We found that neuroblastoma × glioma hybrid NG108-15 cells accumulated lipofuscin-like autofluorescent materials during neuronal differentiation in culture in a medium containing 1% fetal calf serum, 1 mM dibutyryl cyclic AMP and 1 mM theophylline. The emission maximum of the lipofuscin-like autofluorescent materials was between 500 and 550 nm. Granules positive to acid phosphatase and periodic-acid Schiff were increased, as were the autofluorescent granules in NG108-15 cells. Thiolprotease inhibitors, N-(L-3-trans-carboxyoxirane-2-carbonyl)-L-leucine-4-aminobutylamide (E-64) and acetyl-Leu-Leu-Arg (leupeptin), markedly accelerated the accumulation of the lipofuscin-like autofluorescent materials in NG108-15 cells. On the other hand, activities of lysosomal thiolproteases, cathepsin B, C and L, were increased during neuronal differentiation. Protein content in the cells was gradually increased with the neuronal differentiation, and the rise was significantly accelerated when proteolysis was inhibited by E-64. These results suggest that the lipofuscin-like autofluorescent materials contain peptidic substances as a component, and indicate that the increase in hydrolytic activities of thiolproteases during neuronal differentiation is not enough for the hydrolysis of peptidic substrates, resulting in the accumulation of autofluorescent materials in NG108-15 cells.

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Aging-Related Pathology in Human Neuroblastoma and Teratocarcinoma Cell Lines

Cole¹,

Timiras²

1987

Model Systems of Development and Aging of the Nervous System

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Further studies on the effects of centrophenoxine on lipofuscin pigment in neuroblastoma cells in culture: An electron microscopic study

Cited by 16 publications

References 15 publications

Biochemical alterations in rat brain following aluminum exposure: Protection with centrophenoxine

Biochemical alterations in rat brain following aluminum exposure: Protection with centrophenoxine

Formation of Lipofuscin-Like Autofluorescent Materials in NG108-15 Cells: Involvement of Lysosomal Protein Degradation

Aging-Related Pathology in Human Neuroblastoma and Teratocarcinoma Cell Lines

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