Fusarium graminearum forms mycotoxin producing infection structures on wheat

Boenisch, Marike Johanne; Schäfer, Wilhelm

doi:10.1186/1471-2229-11-110

Cited by 237 publications

(211 citation statements)

References 62 publications

Supporting

Mentioning

203

Contrasting

Order By: Relevance

“…This was most likely due to the presence of numerous infection structures that firmly attached the pathogen's hyphae to the surface of kernels, accompanied by very few conidia. The infection structures of F. culmorum observed in our study were similar to those described by Boenisch and Schäfer (2011) who investigated the infection process of F. graminearum. Numerous SEM images show lobate appressoria and infection cushions of F. culmorum-multicellular structures formed by irregularly shaped hyphae.…”

Section: Discussionsupporting

confidence: 87%

“…In addition, trichothecene biosynthesis is induced in the infection structures of F. culmorum. Trichothecenes are able to bind to the 60S ribosomal subunit of eukaryotes, which leads to the inhibition of protein synthesis and induction of apoptosis (Boenisch and Schäfer 2011). Mycotoxins increase the virulence of Fusarium pathogens against cereal plants, and pose a significant health risk for humans and animals.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antagonistic interactions between Aureobasidium pullulans and Fusarium culmorum, a fungal pathogen of winter wheat

Wachowska

Głowacka

2014

BioControl

View full text Add to dashboard Cite

The effect of the biological control agent Aureobasidium pullulans (de Bary) G. Arnaud on the development of Fusarium head blight (FHB) on winter wheat and kernel contamination with fungi of the genera Fusarium, Acremonium, Cladosporium and Penicillium was analyzed in a greenhouse experiment. Scanning electron microscopy was used to evaluate the distribution of A. pullulans cells and aggregates on wheat kernels, infection structures of Fusarium culmorum (W.G. Smith) Sacc and the antagonist-pathogen interactions. Biological control with A. pullulans reduced FHB severity by 21.67 % and improved grain filling by 5.02 %, compared with the control treatment. The survival of A. pullulans was good (to 31 cells per kernel), in particular on the surface and in the crease of kernels, including in pathogen-inoculated wheat plants. A. pullulans cells firmly adhered to F. culmorum hyphae, and damaged them. In most cases, autochthonous communities of filamentous fungi of the genera Acremonium and Penicillium developed at a slower rate after kernel inoculation with the pathogen.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Antagonistic interactions between Aureobasidium pullulans and Fusarium culmorum, a fungal pathogen of winter wheat

Wachowska

Głowacka

2014

BioControl

View full text Add to dashboard Cite

show abstract

“…The trichodiene synthase TRI5 catalyses the first essential step of DON biosynthesis (Hohn and Vanmiddlesworth, 1986). F. graminearum specifically induces DON production in planta, which is associated with the formation of infection structures (Boenisch and Schäfer, 2011). However, it is not yet fully understood how this specific induction is regulated in the fungus.…”

Section: Introductionmentioning

confidence: 99%

High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production

Blum

Benfield

Stiller

et al. 2016

Fungal Genetics and Biology

View full text Add to dashboard Cite

., High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production, Fungal Genetics and Biology (2016), doi: http://dx.doi. org/10.1016/j.fgb. 2016.02.005 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ABSTRACTFusarium head blight and crown rot, caused by the fungal plant pathogen Fusarium graminearum, impose a major threat to global wheat production. During the infection, plants are contaminated with mycotoxins such as deoxynivalenol (DON), which can be toxic for humans and animals. In addition, DON is a major virulence factor during wheat infection. However, it is not fully understood how DON production is regulated in F. graminearum. In order to identify regulators of DON production, a high-throughput mutant screen using Fluorescence Activated Cell Sorting (FACS) of a mutagenized TRI5-GFP reporter strain was established and a mutant over-producing DON under repressive conditions identified. A gain-of-function mutation in the F. graminearum adenylyl cyclase (FAC1), which is a known positive regulator of DON production, was identified as the cause of this phenotype through genome sequencing and segregation analysis. Our results show that the high-throughput mutant screening procedure developed here can be applied for identification of fungal proteins involved in diverse processes.

show abstract

“…After an incubation period of 24 h, we found a large formation of appressoria-like structures and foot structures (Jansen et al, 2005;Boenisch and Schäfer, 2011;Fig. 1, E and F) and decided to take this time point as a starting point for further experiments.…”

Section: Effect Of Z-3-hac Pretreatment On the Severity Of Infection mentioning

confidence: 99%

Priming of Wheat with the Green Leaf Volatile Z-3-Hexenyl Acetate Enhances Defense against Fusarium graminearum But Boosts Deoxynivalenol Production

Ameye

Audenaert²,

Zutter³

et al. 2015

Plant Physiol.

124

117

View full text Add to dashboard Cite

Priming refers to a mechanism whereby plants are sensitized to respond faster and/or more strongly to future pathogen attack. Here, we demonstrate that preexposure to the green leaf volatile Z-3-hexenyl acetate (Z-3-HAC) primed wheat (Triticum aestivum) for enhanced defense against subsequent infection with the hemibiotrophic fungus Fusarium graminearum. Bioassays showed that, after priming with Z-3-HAC, wheat ears accumulated up to 40% fewer necrotic spikelets. Furthermore, leaves of seedlings showed significantly smaller necrotic lesions compared with nonprimed plants, coinciding with strongly reduced fungal growth in planta. Additionally, we found that F. graminearum produced more deoxynivalenol, a mycotoxin, in the primed treatment. Expression analysis of salicylic acid (SA) and jasmonic acid (JA) biosynthesis genes and exogenous methyl salicylate and methyl jasmonate applications showed that plant defense against F. graminearum is sequentially regulated by SA and JA during the early and later stages of infection, respectively. Interestingly, analysis of the effect of Z-3-HAC pretreatment on SA-and JA-responsive gene expression in hormone-treated and pathogen-inoculated seedlings revealed that Z-3-HAC boosts JA-dependent defenses during the necrotrophic infection stage of F. graminearum but suppresses SA-regulated defense during its biotrophic phase. Together, these findings highlight the importance of temporally separated hormone changes in molding plant health and disease and support a scenario whereby the green leaf volatile Z-3-HAC protects wheat against Fusarium head blight by priming for enhanced JA-dependent defenses during the necrotrophic stages of infection.

show abstract

Fusarium graminearum forms mycotoxin producing infection structures on wheat

Cited by 237 publications

References 62 publications

Antagonistic interactions between Aureobasidium pullulans and Fusarium culmorum, a fungal pathogen of winter wheat

Antagonistic interactions between Aureobasidium pullulans and Fusarium culmorum, a fungal pathogen of winter wheat

High-throughput FACS-based mutant screen identifies a gain-of-function allele of the Fusarium graminearum adenylyl cyclase causing deoxynivalenol over-production

Priming of Wheat with the Green Leaf Volatile Z-3-Hexenyl Acetate Enhances Defense against Fusarium graminearum But Boosts Deoxynivalenol Production

Contact Info

Product

Resources

About