FUSARIUM-ID v. 1.0: A DNA Sequence Database for Identifying Fusarium

Plant defense response against Fusarium oxysporum and strategies to develop tolerant genotypes in banana

“…Several DNA-based techniques have been used to analyze the worldwide population of Fox. Classifying Fusarium is always difficult but a FUSARIUM ID v. 1.0, a publicly available database based on partial translation elongation factor 1 alpha (TeF) DNA sequences, which can be used as a phylogenetic marker has been created (Geiser et al 2004). The recent development in the molecular discrimination of Fox was reviewed by Lievens et al (2008).…”

A preliminary DNA barcode selection for the genus Russula (Russulales, Basidiomycota)

“…This gene has previously been regarded as the target DNA barcode in certain groups (Geiser et al 2004;Druzhinina et al 2005;Li et al 2013); however, our analyses showed that tef-1α the occurrence of overlap between intra-and inter-species variation among the candidate genes (Figs. 1, 2 and 7) was the highest for this gene.…”

“…The β-tubulin gene could be used as a suitable DNA barcode for the genera, Aspergillus (Geiser et al 2007;Varga et al 2011), Penicillum (Samson et al 2004), and Tuber (Zampieri et al 2009), but was not suitable for Parmeliaceae and Sordariomycetes (Thell et al 2004;Tang et al 2007). The gene for transcription elongation factor 1-alpha (tef-1α) was suggested as a DNA barcode for the genus Fusarium (Geiser et al 2004), which, along with the second largest RNA polymerase II subunit (rpb2), could precisely distinguish the species of genera Hypocera (Jaklitsch et al 2006) and Neonectria (Zhao et al 2011a;b, Zeng et al 2012). Among the ribosomal RNA genes that are commonly used in molecular phylogenetic analyses, the 18S and 28S rDNA subunits show a high primer commonality; while they were chosen as the DNA barcode for Glomeromycota (Schüßler et al 2001;Schüßler and Walker 2010), they are not appropriate for specific identification because of their low mutation rates (Krüger et al 2009).…”

Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia

“…Therefore, the DNA analysis is needed for accurate identification and characterization of the species. Molecular data in addition to the distinctive morphological characteristics have been used to identify Fusarium species by many authors (Geiser et al, 2004;Wang et al, 2011;Zhu et al, 2014).…”

“…Non-orthologous copies of the gene have not been detected in the genus and universal primers have been designed that barcode successfully amplify this region for all species of the genus (Summerell et al, 2003;Geiser et al, 2004;Kristensen et al, 2005).…”