Future perspectives on aptamer for application in food authentication

Mohamad, Nornazliya; Azizan, Nur Inani; Mokhtar, Nur Fadhilah Khairil; Mustafa, Shuhaimi; Desa, Mohd Nasir Mohd; Hashim, Amalia Mohd

doi:10.1016/j.ab.2022.114861

Cited by 7 publications

(1 citation statement)

References 133 publications

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“…Among these FNAs, aptamers (short-chain DNA or RNA with a specific sequence) are emerging as a novel class of nucleic-acid-based bio-recognition elements in developing biosensors or bioassays to detect various food contaminants [ 15 ], and when those aptamers on the transducer surface bind to a target, electrical or optical signals can be generated for quantification for measuring targets. When aptamers specifically bind to CAP after undergoing adaptive conformational changes [ 16 ], the electrochemical response changes, which is related to the concentration of CAP [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Few Layer Ti3C2 MXene-Based Label-Free Aptasensor for Ultrasensitive Determination of Chloramphenicol in Milk

Li,

Xiong,

Zhao

et al. 2023

Molecules

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Quantitative detection of veterinary drug residues in animal-derived food is of great significance. In this work, a simple and label-free electrochemical aptasensor for the highly sensitive detection of chloramphenicol (CAP) in milk was successfully developed based on a new biosensing method, where the single- or few-layer Ti3C2 MXene nanosheets functionalized via the specific aptamer by self-assembly were used as electrode modifiers for a glassy carbon electrode (aptamer/Ti3C2 MXene/GCE). Differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy (SEM), atomic force microscope (AFM), and so on were utilized for electrochemical and morphological characterization. Under the optimized conditions, the constructed aptasensor exhibited excellent performance with a wider linearity to CAP in the range from 10 fM to 1 μM and a low detection limit of 1 fM. Aptamer/Ti3C2 MXene/GCE demonstrated remarkable selectivity over other potentially interfering antibiotics, as well as exceptional reproducibility and stability. In addition, the aptasensor was successfully applied to determine CAP in milk with acceptable recovery values of 96.13% to 108.15% and relative standard deviations below 9%. Therefore, the proposed electrochemical aptasensor is an excellent alternative for determining CAP in food samples.

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Section: Introductionmentioning

confidence: 99%