Future prospects for tissue engineered lung transplantation

Tsuchiya, Tomoshi; Sivarapatna, Amogh; Rocco, Kevin A.; Nanashima, Atsushi; Nagayasu, Takeshi; Niklason, Laura E.

doi:10.4161/org.27846

Cited by 54 publications

(25 citation statements)

References 111 publications

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“…While ECM-derived scaffolds are thought to contain all of the proteins and growth factors necessary to direct tissue regeneration, care needs to be taken with the decellularization process, since the detergents used can significantly affect the protein and growth factor content within the scaffold [166–169]. Further, incomplete decellularization can result in the persistence of DNA fragments in the material which have the potential to induce an inflammatory response [170].…”

Section: 0 Current Tissue Engineering Strategies and Limitationsmentioning

confidence: 99%

Biomimetic scaffolds for regeneration of volumetric muscle loss in skeletal muscle injuries

et al. 2015

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Skeletal muscle injuries typically result from traumatic incidents such as combat injuries where soft-tissue extremity injuries are present in one of four cases. Further, about 4.5 million reconstructive surgical procedures are performed annually as a result of car accidents, cancer ablation, or cosmetic procedures. These combat- and trauma-induced skeletal muscle injuries are characterized by volumetric muscle loss (VML), which significantly reduces the functionality of the injured muscle. While skeletal muscle has an innate repair mechanism, it is unable to compensate for VML injuries because large amounts of tissue including connective tissue and basement membrane are removed or destroyed. This results in in a significant need to develop off-the-shelf biomimetic scaffolds to direct skeletal muscle regeneration. Here, the structure and organization of native skeletal muscle tissue is described in order to reveal clear design parameters that are necessary for scaffolds to mimic in order to successfully regenerate muscular tissue. We review the literature with respect to the materials and methodologies used to develop scaffolds for skeletal muscle tissue regeneration as well as the limitations of these materials. We further discuss the variety of cell sources and different injury models to provide some context for the multiple approaches used to evaluate these scaffold materials. Recent findings are highlighted to address the state of the field and directions are outlined for future strategies, both in scaffold design and in the use of different injury models to evaluate these materials, for regenerating functional skeletal muscle.

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Section: 0 Current Tissue Engineering Strategies and Limitationsmentioning

confidence: 99%

Biomimetic scaffolds for regeneration of volumetric muscle loss in skeletal muscle injuries

et al. 2015

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“…1-4 Retention of extracellular matrix (ECM) components during the decellularized process is crucial for influencing the behavior of cells that are subsequently placed on the decellularized scaffold. 5 ECM components play a major role in the proper migration, protein expression, and active signaling pathways of the donor cells.…”

Section: Introductionmentioning

confidence: 99%

Sodium hydroxide based non-detergent decellularizing solution for rat lung

et al. 2018

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Lung transplantation is the last option for the treatment of end stage chronic lung disorders. Because the shortage of donor lung organs represents the main hurdle, lung regeneration has been considered to overcome this hurdle. Recellularization of decellularized organ scaffold is a promising option for organ regeneration. Although detergents are ordinarily used for decellularization, other approaches are possible. Here we used high alkaline (pH12) sodium hydroxide (NaOH)-PBS solution without detergents for lung decellularization and compared the efficacy on DNA elimination and ECM preservation with detergent based decellularization solutions CHAPS and SDS. Immunohistochemical image analysis showed that cell components were removed by NaOH solution as well as other detergents. A Collagen and GAG assay showed that the collagen reduction of the NaOH group was comparable to that of the CHAPS and SDS groups. However, DNA reduction was more significant in the NaOH group than in other groups (p < 0.0001). The recellularization of HUVEC revealed cell attachment was not inferior to that of the SDS group. Ex vivo functional analysis showed 100% oxygen ventilation increased oxygen partial pressure as artificial hemoglobin vesicle-PBS solution passed through regenerated lungs in the SDS or NaOH group. It was concluded that the NaOH-PBS based decellularization solution was comparable to ordinal decellularizaton solutions and competitive in cost effectiveness and residues in the decellularized scaffold negligible, thus providing another potential option to detergent for future clinical usage.

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“…In contrast to the idea of engineering in vitro lung mimics (second step), whole lung organ engineering trials arose from the idea of modifying from natural biomaterials. The approach is a cell replacement method using recellularization of decellularized organ scaffolds (Tsuchiya et al, 2014b). Currently, a variety of natural scaffolds are available for clinical usage such as arterial grafts, heart valves, urinary tract reconstitution, skin reconstruction, dura mater grafts following intracranial surgery, and orthopedic applications.…”

Section: Lung Microvascular Niche Reconstruction In Bioengineered Lungmentioning

confidence: 99%