G-quadruplex DNAzyme-based electrochemiluminescence biosensing strategy for VEGF165 detection: Combination of aptamer–target recognition and T7 exonuclease-assisted cycling signal amplification

Zhang, Hui; Li, Meixing; Li, Caihong; Guo, Zhimou; Dong, Huilei; Wu, Ping; Cai, Chenxin

doi:10.1016/j.bios.2015.05.069

Cited by 58 publications

(18 citation statements)

References 45 publications

(63 reference statements)

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“…ECL decrement (Δ I = I 0 – I , where I 0 is the maximum ECL intensity of GCE-ABA-S1-aptamer-CIS/ZnS and I is the maximum ECL intensity of GCE-ABA-S1-aptamer-CIS/ZnS-VEGF 165 ) increased linearly with the logarithmically increasing concentration of VEGF 165 in a range from 0.10 to 1000 pM (inset, Figure , R = 0.99) with a limit of detection of 0.050 pM (S/N = 3). GCE-ABA-S1-aptamer-CIS/ZnS-VEGF 165 not only displayed comparable or even better sensitivity than previously reported VEGF 165 determining strategies (Tables S2) ,− but also demonstrated acceptable selectivity to common interfering substances such as BSA, ascorbic acid, and thrombin (Figure S4). The desired selectivity of the proposed ECL sensor for VEGF 165 was derived from the bioselective reaction between DNA capture probe S1, VEGF 165 aptamer, and VEGF 165 …”

Section: Resultsmentioning

confidence: 60%

“…HPLC-purified DNA capture probe S1, VEGF 165 aptamer, and VEGF 165 were purchased from Shanghai Sangon Biological Engineering Technology and Services Co. Ltd. (Shanghai, China). VEGF 165 aptamer was modified with a carboxyl group at the 5′-terminus and its sequence is as follows: 5′-COOH-GGCCCGTCCGTATGGTGGGTGTGCTGGCC-3′ . DNA capture probe S1 was modified at the 5′-terminus with an amino group and its sequence is as follows: 5′-NH 2 -TTTTTTTATGGGTTGGGCGGGATGGGCCAGCACACCCACC-3′ .…”

Section: Experimental Sectionmentioning

confidence: 99%

“…VEGF 165 aptamer was modified with a carboxyl group at the 5′-terminus and its sequence is as follows: 5′-COOH-GGCCCGTCCGTATGGTGGGTGTGCTGGCC-3′ . DNA capture probe S1 was modified at the 5′-terminus with an amino group and its sequence is as follows: 5′-NH 2 -TTTTTTTATGGGTTGGGCGGGATGGGCCAGCACACCCACC-3′ . All the samples were prepared with 0.10 M phosphate buffer (PB, pH 7.4) and stored at 4 °C before use.…”

Section: Experimental Sectionmentioning

confidence: 99%

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Promising Anodic Electrochemiluminescence of Nontoxic Core/Shell CuInS₂/ZnS Nanocrystals in Aqueous Medium and Its Biosensing Potential

et al. 2018

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Copper indium sulfide (CuInS, CIS) nanocrystals (NCs) are a promising solution to the toxic issue of Cd- and Pb-based NCs. Herein, electrochemiluminescence (ECL) of CIS NCs in aqueous medium is investigated for the first time with l-glutathione and sodium citrate-stabilized water-soluble CIS/ZnS NCs as model. The CIS/ZnS NCs can be oxidized to hole-injected states via electrochemically injecting holes into valence band at 0.55 and 0.94 V (vs Ag/AgCl), respectively. The hole-injected state around 0.94 V can bring out efficient oxidative-reduction ECL with a similar color to Ru(bpy) in the presence of tri- n-propylamine (TPrA) and enable CIS/ZnS NCs promising ECL tags with l-glutathione as linker for labeling. The ECL of CIS/ZnS NCs/TPrA can be utilized to determine vascular endothelial growth factor (VEGF) from 0.10 to 1000 pM with the limit of detection at 0.050 pM (S/N = 3). Although the hole-injected state around 0.55 V is generated ahead of oxidation of TPrA and fails to bring out coreactant ECL, annihilation ECL proves that both hole-injected states generated, at 0.55 and 0.94 V, can be involved in electrochemical redox-induced radiative charge transfer by directly stepping CIS/ZnS NCs from electron-injecting potential to hole-injecting potential. CIS/ZnS NCs are promising nontoxic electrochemiluminophores with lowered ECL triggering potential around 0.55 V for less electrochemical interference upon the development of coreactant.

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Section: Resultsmentioning

confidence: 60%

Section: Experimental Sectionmentioning

confidence: 99%

Section: Experimental Sectionmentioning

confidence: 99%

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Promising Anodic Electrochemiluminescence of Nontoxic Core/Shell CuInS₂/ZnS Nanocrystals in Aqueous Medium and Its Biosensing Potential

et al. 2018

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“…Using systematic evolution of ligands by exponential enrichment (SELEX) strategies, a variety of VEGF 165 and CEA aptamers have been identified by previous studies. [36][37][38] Referring to the existing studies of VEGF 165 and CEA aptamers, the aptamer sequences designed in this study are shown in Table 1. [39][40][41][42] The VEGF 165 and CEA aptamers have been proved to bind to their corresponding targets with high affinity, respectively.…”

Section: Introductionmentioning

confidence: 99%

<p>Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay</p>

Mu¹,

Dong²,

Wang³

et al. 2020

IJN

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Background As two important tumor markers, vascular endothelial growth factor (VEGF) and carcinoembryonic antigen (CEA) have a great value for clinical application in the early diagnosis of cancer. Due to the complex composition of biological samples, the results from combined detection of CEA and VEGF are often taken as a comprehensive indicator in order to make an accurate judgment on a disease. However, most of the current methods can only be used to detect the content of one biomarker. Therefore, it is necessary to explore a simple, rapid, low-cost, and highly sensitive method for the simultaneous detection of CEA and VEGF. Methods Based on specific aptamers and magnetic separation, a time-resolved chemiluminescence enzyme-linked aptamer assay was developed for the simultaneous detections of CEA and VEGF in serum samples. Results Under the optimal conditions, the linear range of the calibration curve for VEGF was from 0.5 to 80 ng mL −1 , and the limit of detection was 0.1 ng mL −1 . The linear range of the calibration curve for CEA was 0.5 to 160 ng mL −1 , and the limit of detection was 0.1 ng mL −1 . The established method was applied to detect VEGF and CEA in serum samples. The results were consistent with those of commercial kits. Conclusion The method has high sensitivity and can quickly obtain accurate results, which could greatly improve the measurement efficiency, reduce the cost, and also reduce the volume of sample consumed. It can be seen that the method established in this study has important application value and broad application prospect in clinical diagnosis.

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“… 19 Therefore, they are regarded as an alternative to antibody-based methods. Up to now, a variety of aptamer-based analytical strategies for the detection of VEGF 165 have been reported, such as differential pulse voltammetry (DPV), 20,21 electrochemical impedance spectroscopy (EIS), 11 photoelectrochemical (PEC), 22 electrochemiluminescence (ECL), 23 chemiluminescence (CL), 24,25 and time-resolved fluorescence (TR-FL). 26 These methods have good specificity and stability compared to traditional approaches.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive enzyme-free fluorescent detection of VEGF₁₆₅ based on target-triggered hybridization chain reaction amplification

et al. 2018

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G-quadruplex DNAzyme-based electrochemiluminescence biosensing strategy for VEGF165 detection: Combination of aptamer–target recognition and T7 exonuclease-assisted cycling signal amplification

Cited by 58 publications

References 45 publications

Promising Anodic Electrochemiluminescence of Nontoxic Core/Shell CuInS₂/ZnS Nanocrystals in Aqueous Medium and Its Biosensing Potential

Promising Anodic Electrochemiluminescence of Nontoxic Core/Shell CuInS₂/ZnS Nanocrystals in Aqueous Medium and Its Biosensing Potential

<p>Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay</p>

Ultrasensitive enzyme-free fluorescent detection of VEGF₁₆₅ based on target-triggered hybridization chain reaction amplification

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G-quadruplex DNAzyme-based electrochemiluminescence biosensing strategy for VEGF165 detection: Combination of aptamer–target recognition and T7 exonuclease-assisted cycling signal amplification

Cited by 58 publications

References 45 publications

Promising Anodic Electrochemiluminescence of Nontoxic Core/Shell CuInS2/ZnS Nanocrystals in Aqueous Medium and Its Biosensing Potential

Promising Anodic Electrochemiluminescence of Nontoxic Core/Shell CuInS2/ZnS Nanocrystals in Aqueous Medium and Its Biosensing Potential

<p>Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay</p>

Ultrasensitive enzyme-free fluorescent detection of VEGF165 based on target-triggered hybridization chain reaction amplification

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Promising Anodic Electrochemiluminescence of Nontoxic Core/Shell CuInS₂/ZnS Nanocrystals in Aqueous Medium and Its Biosensing Potential

Promising Anodic Electrochemiluminescence of Nontoxic Core/Shell CuInS₂/ZnS Nanocrystals in Aqueous Medium and Its Biosensing Potential

Ultrasensitive enzyme-free fluorescent detection of VEGF₁₆₅ based on target-triggered hybridization chain reaction amplification